AB172793

Anti-NSMase2 antibody

Name: Anti-NSMase2 antibody (ab172793) | Abcam
Brand: Abcam Limited
SKU: AB172793

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal NSMase2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Sphingomyelin phosphodiesterase 3.

View Alternative Names

Sphingomyelin phosphodiesterase 3, Neutral sphingomyelinase 2, Neutral sphingomyelinase II, nSMase-2, nSMase2, SMPD3

1 Images

Supplier Data

Western blot - Anti-NSMase2 antibody (AB172793)

All lanes:

Western blot - Anti-NSMase2 antibody (ab172793) at 1 µg/mL

Lane 1:

NSMase2 transfected 293T cell line lysate at 15 µL

Lane 2:

Non-transfected 293T cell line lysate at 15 µL

Predicted band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Sphingomyelin phosphodiesterase 3.

Q9NY59

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSMase2 also known as neutral sphingomyelinase 2 is an enzyme with a molecular mass of approximately 97 kDa. It catalyzes the hydrolysis of sphingomyelin into ceramide and phosphorylcholine. NSMase2 is expressed in various tissues like brain liver and kidney. The enzyme is membrane-associated where it plays a role in the regulation of sphingolipid metabolism.

Biological function summary

NSMase2 influences many cellular processes by controlling levels of bioactive lipids. It is an important participant in the ceramide production pathway impacting cell signaling and apoptosis. While not usually part of large complexes its activity modulates lipid raft composition influencing membrane-associated signaling pathways.

Pathways

NSMase2 contributes significantly to the sphingolipid metabolic pathway by generating ceramide which acts as a second messenger in various cellular responses. Notably it interfaces with the MAPK pathway affecting signal transduction related to stress and inflammation. It has functional relations with proteins like PKC which is involved in cell survival and apoptosis processes.

NSMase2 has been linked to neurodegenerative diseases like Alzheimer's disease and the inflammatory disorder atherosclerosis. Dysregulation in NSMase2 activity can result in altered ceramide levels contributing to these conditions. Additionally its interaction with TNFα a pro-inflammatory cytokine exemplifies its involvement in inflammatory responses and related diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization (PubMed : 10823942, PubMed : 14741383, PubMed : 15051724). Binds to anionic phospholipids (APLs) such as phosphatidylserine (PS) and phosphatidic acid (PA) that modulate enzymatic activity and subcellular location. May be involved in IL-1-beta-induced JNK activation in hepatocytes (By similarity). May act as a mediator in transcriptional regulation of NOS2/iNOS via the NF-kappa-B activation under inflammatory conditions (By similarity).

See full target information Sphingomyelin phosphodiesterase 3

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Animal models and experimental medicine 5:461-469 PubMed36168146

2022

DNA polymerase delta interacting protein 3 facilitates the activation and maintenance of DNA damage checkpoint in response to replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Sufang Zhang,Ernest Y C Lee,Marietta Y W T Lee,Dong Zhang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NSMase2 antibody

Western blot - Anti-NSMase2 antibody (AB172793)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise