AB199399

Anti-NSMase2 antibody

Name: Anti-NSMase2 antibody (ab199399) | Abcam
Brand: Abcam Limited
SKU: AB199399

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(2 Publications)

Rabbit Polyclonal NSMase2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Sphingomyelin phosphodiesterase 3 aa 350-600.

View Alternative Names

Sphingomyelin phosphodiesterase 3, Neutral sphingomyelinase 2, Neutral sphingomyelinase II, nSMase-2, nSMase2, SMPD3

1 Images

Supplier Data

Western blot - Anti-NSMase2 antibody (AB199399)

All lanes:

Western blot - Anti-NSMase2 antibody (ab199399) at 1/500 dilution

All lanes:

Human fetal brain lysate

Predicted band size: 71 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human Sphingomyelin phosphodiesterase 3 aa 350-600. The exact immunogen used to generate this antibody is proprietary information.

Q9NY59

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Reactivity data

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSMase2 also known as neutral sphingomyelinase 2 is an enzyme with a molecular mass of approximately 97 kDa. It catalyzes the hydrolysis of sphingomyelin into ceramide and phosphorylcholine. NSMase2 is expressed in various tissues like brain liver and kidney. The enzyme is membrane-associated where it plays a role in the regulation of sphingolipid metabolism.

Biological function summary

NSMase2 influences many cellular processes by controlling levels of bioactive lipids. It is an important participant in the ceramide production pathway impacting cell signaling and apoptosis. While not usually part of large complexes its activity modulates lipid raft composition influencing membrane-associated signaling pathways.

Pathways

NSMase2 contributes significantly to the sphingolipid metabolic pathway by generating ceramide which acts as a second messenger in various cellular responses. Notably it interfaces with the MAPK pathway affecting signal transduction related to stress and inflammation. It has functional relations with proteins like PKC which is involved in cell survival and apoptosis processes.

NSMase2 has been linked to neurodegenerative diseases like Alzheimer's disease and the inflammatory disorder atherosclerosis. Dysregulation in NSMase2 activity can result in altered ceramide levels contributing to these conditions. Additionally its interaction with TNFα a pro-inflammatory cytokine exemplifies its involvement in inflammatory responses and related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization (PubMed : 10823942, PubMed : 14741383, PubMed : 15051724). Binds to anionic phospholipids (APLs) such as phosphatidylserine (PS) and phosphatidic acid (PA) that modulate enzymatic activity and subcellular location. May be involved in IL-1-beta-induced JNK activation in hepatocytes (By similarity). May act as a mediator in transcriptional regulation of NOS2/iNOS via the NF-kappa-B activation under inflammatory conditions (By similarity).

See full target information Sphingomyelin phosphodiesterase 3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nan fang yi ke da xue xue bao = Journal of Southern Medical University 43:710-717 PubMed37313811

2023

[RITA selectively inhibits proliferation of -deficient cutaneous melanoma cells ].

Applications

Unspecified application

Species

Unspecified reactive species

W Shi,X Liu,G Zhang,L Ye,R Zhou,Y Li,L Yu

International journal of molecular sciences 20: PubMed31766565

2019

In Human and Mouse Spino-Cerebellar Tissue, Ataxin-2 Expansion Affects Ceramide-Sphingomyelin Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Nesli-Ece Sen,Aleksandar Arsovic,David Meierhofer,Susanne Brodesser,Carola Oberschmidt,Júlia Canet-Pons,Zeynep-Ece Kaya,Melanie-Vanessa Halbach,Suzana Gispert,Konrad Sandhoff,Georg Auburger

View all publications

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Product promise

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Anti-NSMase2 antibody

Western blot - Anti-NSMase2 antibody (AB199399)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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