AB68735

Anti-NSMase2 antibody

BOND RX™ Validated
What is this?

Be the first to review this product! Submit a review

(4 Publications)

Mouse Polyclonal NSMase2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Sphingomyelin phosphodiesterase 3.

View Alternative Names

Sphingomyelin phosphodiesterase 3, Neutral sphingomyelinase 2, Neutral sphingomyelinase II, nSMase-2, nSMase2, SMPD3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMase2 antibody (AB68735)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMase2 antibody (AB68735)

IHC image of ab68735 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68735, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-NSMase2 antibody (AB68735)

All lanes:

Western blot - Anti-NSMase2 antibody (ab68735) at 1/500 dilution

Lane 1:

nSMase2 transfected 293T cell lysate at 25 µg

Lane 2:

Non transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 71 kDa

Observed band size: 78 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Sphingomyelin phosphodiesterase 3.

Q9NY59

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSMase2 also known as neutral sphingomyelinase 2 is an enzyme with a molecular mass of approximately 97 kDa. It catalyzes the hydrolysis of sphingomyelin into ceramide and phosphorylcholine. NSMase2 is expressed in various tissues like brain liver and kidney. The enzyme is membrane-associated where it plays a role in the regulation of sphingolipid metabolism.

Biological function summary

NSMase2 influences many cellular processes by controlling levels of bioactive lipids. It is an important participant in the ceramide production pathway impacting cell signaling and apoptosis. While not usually part of large complexes its activity modulates lipid raft composition influencing membrane-associated signaling pathways.

Pathways

NSMase2 contributes significantly to the sphingolipid metabolic pathway by generating ceramide which acts as a second messenger in various cellular responses. Notably it interfaces with the MAPK pathway affecting signal transduction related to stress and inflammation. It has functional relations with proteins like PKC which is involved in cell survival and apoptosis processes.

NSMase2 has been linked to neurodegenerative diseases like Alzheimer's disease and the inflammatory disorder atherosclerosis. Dysregulation in NSMase2 activity can result in altered ceramide levels contributing to these conditions. Additionally its interaction with TNFα a pro-inflammatory cytokine exemplifies its involvement in inflammatory responses and related diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization (PubMed : 10823942, PubMed : 14741383, PubMed : 15051724). Binds to anionic phospholipids (APLs) such as phosphatidylserine (PS) and phosphatidic acid (PA) that modulate enzymatic activity and subcellular location. May be involved in IL-1-beta-induced JNK activation in hepatocytes (By similarity). May act as a mediator in transcriptional regulation of NOS2/iNOS via the NF-kappa-B activation under inflammatory conditions (By similarity).

See full target information Sphingomyelin phosphodiesterase 3

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 42:e113105 PubMed37409525

2023

TECPR1 is activated by damage-induced sphingomyelin exposure to mediate noncanonical autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Namrita Kaur,Laura Rodriguez de la Ballina,Håvard Styrkestad Haukaas,Maria Lyngaas Torgersen,Maja Radulovic,Michael J Munson,Alan Sabirsh,Harald Stenmark,Anne Simonsen,Sven R Carlsson,Alf Håkon Lystad

Frontiers in oncology 11:711448 PubMed34888230

2021

Norcholic Acid Promotes Tumor Progression and Immune Escape by Regulating Farnesoid X Receptor in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yihang Gong,Kun Li,Yunfei Qin,Kaining Zeng,Jianrong Liu,Shaozhuo Huang,Yewu Chen,Haoyuan Yu,Wei Liu,Linsen Ye,Yang Yang

Nature communications 11:1211 PubMed32139701

2020

Primary tumors release ITGBL1-rich extracellular vesicles to promote distal metastatic tumor growth through fibroblast-niche formation.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Ji,Lihong Zhou,Hua Sui,Liu Yang,Xinnan Wu,Qing Song,Ru Jia,Ruixiao Li,Jian Sun,Ziyuan Wang,Ningning Liu,Yuanyuan Feng,Xiaoting Sun,Gang Cai,Yu Feng,Jianfeng Cai,Yihai Cao,Guoxiang Cai,Yan Wang,Qi Li

Nature communications 10:1387 PubMed30918259

2019

Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Ju Im,Chan-Hyeong Lee,Pyong-Gon Moon,Gunassekaran Gowri Rangaswamy,Byungheon Lee,Jae Man Lee,Jae-Chul Lee,Jun-Goo Jee,Jong-Sup Bae,Taeg-Kyu Kwon,Keon-Wook Kang,Myeong-Seon Jeong,Joo-Eun Lee,Hyun-Suk Jung,Hyun-Joo Ro,Sangmi Jun,Wonku Kang,Seung-Yong Seo,Young-Eun Cho,Byoung-Joon Song,Moon-Chang Baek

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NSMase2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMase2 antibody (AB68735)

Western blot - Anti-NSMase2 antibody (AB68735)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise