Mouse Polyclonal NSMase2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Sphingomyelin phosphodiesterase 3.
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Publications
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- Frontiers in oncology 11:7114482021Norcholic Acid Promotes Tumor Progression and Immune Escape by Regulating Farnesoid X Receptor in Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYihang Gong,Kun Li,Yunfei Qin,Kaining Zeng,Jianrong Liu,Shaozhuo Huang,Yewu Chen,Haoyuan Yu,Wei Liu,Linsen Ye,Yang YangPubMed 34888230
- Nature communications 11:12112020Primary tumors release ITGBL1-rich extracellular vesicles to promote distal metastatic tumor growth through fibroblast-niche formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Ji et. al.PubMed 32139701
- Nature communications 10:13872019Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun-Ju Im et. al.PubMed 30918259
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human Sphingomyelin phosphodiesterase 3. Database link Q9NY59
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization (PubMed:10823942, PubMed:14741383, PubMed:15051724). Binds to anionic phospholipids (APLs) such as phosphatidylserine (PS) and phosphatidic acid (PA) that modulate enzymatic activity and subcellular location. May be involved in IL-1-beta-induced JNK activation in hepatocytes (By similarity). May act as a mediator in transcriptional regulation of NOS2/iNOS via the NF-kappa-B activation under inflammatory conditions (By similarity).
Alternative names
Sphingomyelin phosphodiesterase 3, Neutral sphingomyelinase 2, Neutral sphingomyelinase II, nSMase-2, nSMase2, SMPD3
Recommended products
Mouse Polyclonal NSMase2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Sphingomyelin phosphodiesterase 3.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human Sphingomyelin phosphodiesterase 3. Database link Q9NY59
- Carrier free
- Yes
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NSMase2 also known as neutral sphingomyelinase 2 is an enzyme with a molecular mass of approximately 97 kDa. It catalyzes the hydrolysis of sphingomyelin into ceramide and phosphorylcholine. NSMase2 is expressed in various tissues like brain liver and kidney. The enzyme is membrane-associated where it plays a role in the regulation of sphingolipid metabolism.
- Biological function summary
NSMase2 influences many cellular processes by controlling levels of bioactive lipids. It is an important participant in the ceramide production pathway impacting cell signaling and apoptosis. While not usually part of large complexes its activity modulates lipid raft composition influencing membrane-associated signaling pathways.
- Pathways
NSMase2 contributes significantly to the sphingolipid metabolic pathway by generating ceramide which acts as a second messenger in various cellular responses. Notably it interfaces with the MAPK pathway affecting signal transduction related to stress and inflammation. It has functional relations with proteins like PKC which is involved in cell survival and apoptosis processes.
- Associated diseases and disorders
NSMase2 has been linked to neurodegenerative diseases like Alzheimer's disease and the inflammatory disorder atherosclerosis. Dysregulation in NSMase2 activity can result in altered ceramide levels contributing to these conditions. Additionally its interaction with TNFα a pro-inflammatory cytokine exemplifies its involvement in inflammatory responses and related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-NSMase2 antibody (ab68735)
All lanes: Western blot - Anti-NSMase2 antibody (ab68735) at 1/500 dilution
Lane 1: nSMase2 transfected 293T cell lysate at 25 µg
Lane 2: Non transfected 293T cell lysate at 25 µg
SecondaryAll lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 71 kDa
Observed band size: 78 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMase2 antibody (ab68735)
IHC image of ab68735 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68735, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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