AB168578

Anti-NSMCE1 antibody

Name: Anti-NSMCE1 antibody (ab168578) | Abcam
Brand: Abcam Limited
SKU: AB168578

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(1 Publication)

Mouse Polyclonal NSMCE1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NSMCE1.

View Alternative Names

HSPC333, HSPC337, NSMCE1, Non-structural maintenance of chromosomes element 1 homolog, Non-SMC element 1 homolog

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NSMCE1 antibody (AB168578)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NSMCE1 antibody (AB168578)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling NSMCE1 using ab168578 at 10 µg/ml.

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Western blot - Anti-NSMCE1 antibody (AB168578)

All lanes:

Western blot - Anti-NSMCE1 antibody (ab168578) at 1 µg/mL

Lane 1:

NSMCE1-transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG

Predicted band size: 31 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NSMCE1.

Q8WV22

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NSMCE1 protein also known as non-SMC element 1 homolog is an essential component of the SMC5-SMC6 complex. It has a molecular mass of approximately 25 kDa and plays a pivotal mechanical role in DNA repair and replication processes. NSMCE1 expresses in various tissues showing higher levels in proliferative cells due to its need in maintaining genomic stability. This protein interacts with other structural maintenance of chromosome (SMC) proteins to facilitate these vital cellular functions.

Biological function summary

DNA repair and replication depend on NSMCE1 as it participates in the SMC5-SMC6 complex. This complex functions effectively in regulating chromosome segregation and maintaining genomic integrity during the cell cycle. Without NSMCE1 cells exhibit impaired repair of DNA double-strand breaks and defects in the homologous recombination pathway leading to genomic instability. The complex itself requires NSMCE1 to ensure accurate cell division and prevent harmful mutations.

Pathways

NSMCE1 engages heavily in the DNA damage response and homologous recombination pathways. Its interaction is important with the SMC5-SMC6 complex which collaborates with proteins like RAD51 and BRCA1 during DNA repair mechanisms. These pathways play an important role in the repair of DNA breaks safeguarding the genome against potential damage. NSMCE1 through these interactions helps preserve genetic integrity by ensuring the proper repair and replication of DNA under stress conditions.

Defects in NSMCE1 expression associates with cancer and genomic instability disorders. Its impairment could disrupt the function of the SMC5-SMC6 complex leading to increased susceptibility to cancers due to flawed DNA repair mechanisms. NSMCE1 shows links to BRCA1-related breast cancer due to its role in the homologous recombination pathway. By understanding NSMCE1's involvement in these diseases research moves closer to developing targeted therapies for conditions stemming from its dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RING-type zinc finger-containing E3 ubiquitin ligase that assembles with melanoma antigen protein (MAGE) to catalyze the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. Within MAGE-RING ubiquitin ligase complex, MAGE stimulates and specifies ubiquitin ligase activity likely through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3 : substrate complex. Involved in maintenance of genome integrity, DNA damage response and DNA repair (PubMed : 20864041, PubMed : 29225034). NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair (PubMed : 18086888). MAGEF1-NSMCE1 ubiquitin ligase promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors (PubMed : 29225034).

See full target information NSMCE1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 15:845875 PubMed35465095

2022

Spinal dI4 Interneuron Differentiation From Human Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Xu,Liang-Jiang Huang,Zhengyu Fang,Hong-Mei Luo,Yun-Qiang Chen,Ya-Jie Li,Chen-Zi Gong,Hong Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NSMCE1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NSMCE1 antibody (AB168578)

Western blot - Anti-NSMCE1 antibody (AB168578)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise