AB87801

Anti-NSP 5 alpha 3 alpha antibody

Name: Anti-NSP 5 alpha 3 alpha antibody (ab87801) | Abcam
Brand: Abcam Limited
SKU: AB87801
Price: 520 USD
Availability: InStock
Rating: 4 (1 reviews)

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Rabbit Polyclonal NSP 5 alpha 3 alpha antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SPECC1 aa 450-500.

View Alternative Names

CYTSB, NSP5, SPECC1, Cytospin-B, Nuclear structure protein 5, Sperm antigen HCMOGT-1, Sperm antigen with calponin homology and coiled-coil domains 1

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Immunoprecipitation - Anti-NSP 5 alpha 3 alpha antibody (AB87801)

Detection of NSP 5 alpha 3 alpha in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87801 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-NSP 5 alpha 3 alpha antibody (ab87801)

Predicted band size: 119 kDa

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Western blot - Anti-NSP 5 alpha 3 alpha antibody (AB87801)

All lanes:

Western blot - Anti-NSP 5 alpha 3 alpha antibody (ab87801) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 119 kDa

Observed band size: 119 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human SPECC1 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

Q5M775

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab87801 was affinity purified using an epitope specific to NSP 5 alpha 3 alpha immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NSP5 alpha 3 alpha protein also known as non-structural protein 5 is an important player in viral replication. This protein weighs approximately 32 kilodaltons and is expressed primarily in cells infected by certain types of viruses including coronaviruses. NSP5 alpha 3 alpha functions as a protease which is an enzyme that cleaves other proteins to facilitate the viral replication process. It is often considered the main protease of the virus essential for processing the viral polyprotein into functional units necessary for the virus lifecycle.

Biological function summary

The NSP5 alpha 3 alpha protein acts as a pivotal component of the viral replication machinery. It is a critical member of the viral replication complex which consists of non-structural proteins that work together to produce viral RNA. These complexes form in the cytoplasm of infected host cells and coordinate the replication and transcription of the viral genome. The activity of NSP5 alpha 3 alpha protease cleaves the viral polyprotein at specific sites releasing other non-structural proteins that perform various roles in replication.

Pathways

NSP5 alpha 3 alpha protein plays a central role in the viral RNA synthesis pathway. Its proteolytic activity is essential for the maturation of several non-structural proteins that participate in RNA replication and transcription. NSP5 alpha 3 alpha functions closely with NSP12 an important polymerase enzyme within the viral RNA-dependent RNA polymerase complex. Together these proteins ensure continuous and efficient viral RNA synthesis which is important for successful viral propagation within the host.

NSP5 alpha 3 alpha protein is intimately linked to respiratory diseases such as COVID-19. The protein's role in viral replication makes it a significant target for developing antiviral therapies aimed at inhibiting its protease activity. The NSP5 alpha 3 alpha protein also connects to other viral proteins such as NSP3 and NSP16 which are implicated in immune evasion mechanisms that allow the virus to persist and cause severe disease symptoms. Understanding the interactions of NSP5 alpha 3 alpha with these proteins may provide new insights into therapeutic approaches for combatting these infections.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information SPECC1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NSP 5 alpha 3 alpha antibody

Immunoprecipitation - Anti-NSP 5 alpha 3 alpha antibody (AB87801)

Western blot - Anti-NSP 5 alpha 3 alpha antibody (AB87801)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Product promise