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AB250459

Anti-NSP3 antibody [EPR13493] - BSA and Azide free

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Rabbit Recombinant Monoclonal NSP3 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

NSP3, UNQ272/PRO309/PRO34088, SH2D3C, SH2 domain-containing protein 3C, Cas/HEF1-associated signal transducer, Novel SH2-containing protein 3, SH2 domain-containing Eph receptor-binding protein 1, Chat-H, SHEP1

1 Images
Western blot - Anti-NSP3 antibody [EPR13493] - BSA and Azide free (AB250459)
  • WB

Supplier Data

Western blot - Anti-NSP3 antibody [EPR13493] - BSA and Azide free (AB250459)

This data was developed using ab181246, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NSP3 antibody [EPR13493] (<a href='/en-us/products/primary-antibodies/nsp3-antibody-epr13493-ab181246'>ab181246</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

U87-MG cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 94 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13493

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250459 is the carrier-free version of ab181246.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSP3 also known as non-structural protein 3 is a protein involved in several mechanical processes within viral replication. This protein has a mass of roughly 219 kDa and is expressed in cells infected by certain viruses like the SARS-CoV-2 virus responsible for COVID-19. Mechanically NSP3 functions by interacting with host cell translation machinery and is important for the processing of viral polyproteins. NSP3 contains multiple domains including a papain-like protease (PLpro) domain that play roles in modifying host and viral proteins to assist in viral replication and pathogenesis.
Biological function summary

This protein plays significant roles in immune evasion and replication of viral RNA. NSP3 is a component of the viral replication transcription complex which collaborates with other non-structural proteins such as NSP4 and NSP6. Its PLpro domain exhibits deubiquitinating activity which helps the virus undermine host immune responses. These activities are important for the efficiency of viral replication and the persistence of infection within the host.

Pathways

NSP3 fits into complex viral replication and immune evasion pathways. It significantly impacts the ubiquitin-proteasome pathway by interfering with the host's ubiquitin signaling. NSP3 also involves in pathways related to protein processing in the endoplasmic reticulum where it interacts with NSP4 and NSP5 to facilitate the formation of double-membrane vesicles vital for RNA synthesis.

NSP3 has strong associations with viral infections like COVID-19. Its interactions with host proteins such as interferon-stimulated genes make it a target for potential therapeutic interventions. The NSP3 protein is also explored for its role in coronavirus-induced respiratory illnesses where its modulation could influence disease progression and severity. Knowledge of its function can contribute to developing treatments that aim to inhibit NSP3 activity offering potential management strategies for viral-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as an adapter protein that mediates cell signaling pathways involved in cellular functions such as cell adhesion and migration, tissue organization, and the regulation of the immune response (PubMed : 12432078, PubMed : 20881139). Plays a role in integrin-mediated cell adhesion through BCAR1-CRK-RAPGEF1 signaling and activation of the small GTPase RAP1 (PubMed : 12432078). Promotes cell migration and invasion through the extracellular matrix (PubMed : 20881139). Required for marginal zone B-cell development and thymus-independent type 2 immune responses (By similarity). Mediates migration and adhesion of B cells in the splenic marginal zone via promoting hyperphosphorylation of NEDD9/CASL (By similarity). Plays a role in CXCL13-induced chemotaxis of B-cells (By similarity). Plays a role in the migration of olfactory sensory neurons (OSNs) into the forebrain and the innervation of the olfactory bulb by the OSN axons during development (By similarity). Required for the efficient tyrosine phosphorylation of BCAR1 in OSN axons (By similarity).. Isoform 1. Important regulator of chemokine-induced, integrin-mediated T lymphocyte adhesion and migration, acting upstream of RAP1 (By similarity). Required for tissue-specific adhesion of T lymphocytes to peripheral tissues (By similarity). Required for basal and CXCL2 stimulated serine-threonine phosphorylation of NEDD9 (By similarity). May be involved in the regulation of T-cell receptor-mediated IL2 production through the activation of the JNK pathway in T-cells (By similarity).. Isoform 2. May be involved in the BCAR1/CAS-mediated JNK activation pathway.
See full target information SH2D3C
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Product promise

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