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AB4642

Anti-Nsr1p antibody [31C4]

5

(2 Reviews)

|

(4 Publications)

Mouse Monoclonal NSR1 antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 4 publications.

View Alternative Names

YGR159C, G7001, NSR1, Nuclear localization sequence-binding protein, p67

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (AB4642)
  • ICC/IF

AbReview20738****

Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (AB4642)

ab4642 (1/5000) detecting Nsr1p in Saccharomyces cerevisae DDY1810 (red). Cells were fixed in paraformaldehyde, permeabilized with methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue).
Right hand panel : negative control performed using Saccharomyces cerevisae DDY1810 nsr1p knockout.
For further experimental details please refer to Abreview.

Image courtesy of an anonymous Abreview.

Western blot - Anti-Nsr1p antibody [31C4] (AB4642)
  • WB

AbReview20737****

Western blot - Anti-Nsr1p antibody [31C4] (AB4642)

Bottom panel : loading control performed using Saccharomyces cerevisae anti-HSP60 (1 : 1000)

All lanes:

Western blot - Anti-Nsr1p antibody [31C4] (ab4642) at 1/10000 dilution

Lane 1:

Saccharomyces cerevisae DDY1810 (WT) whole cell lysate

Lane 2:

Saccharomyces cerevisae DDY1810 (<i>nsr1</i> knockout) whole cell lysate

Secondary

All lanes:

Rabbit anti-mouse IgG conjugated to peroxidase at 1/30000 dilution

Predicted band size: 45 kDa

false

Image courtesy of an anonymous Abreview.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

31C4

Isotype

IgG

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

WB, ICC/IF

applications

Specificity

This antibody was isolated during a screen for mAbs against yeast nucleolar proteins. Specificity was determined by screening a yeast genomic library in lambda gt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones beta-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with …EFEH190 in Nsr1p. Thus, it is likely to react with an epitope(s) in the C-terminal 55% of Nsr1p.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>For non-ECL western detection methods, 1/1000 - 1/5000.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/5000", "ICCIF-species-notes": "<p>(yeast cells).</p>" } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
Sterile filtered.
Storage buffer
Preservative: 0.065% Sodium azide Constituents: Tissue culture supernatant
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nsr1p also known as Nop1/Nsr1 is a nucleolar protein involved in ribosome biosynthesis. It plays an essential mechanical role in the processing of pre-rRNA and is critical for the assembly of ribosomal subunits. Nsr1p weighs approximately 51 kDa and has a characteristic migration pattern due to its unique structure and binding properties. It expresses mainly in the nucleolus where it is responsible for binding to nucleic acids and contributes to ribosome assembly by mediating the interactions between different rRNA precursors and ribosomal proteins.
Biological function summary

Nsr1p functions as an important player in ribosomal RNA processing and synthesis. This protein associates with multiple enzymes and other nucleic acid-associated factors forming a complex that stabilizes and processes the rRNA transcripts into mature rRNA species. Its role in the nucleolus facilitates the transcriptional processing of rRNA therefore supporting ribosome production. Without Nsr1p ribosome assembly would be insufficient leading to disrupted protein synthesis in cells.

Pathways

Nsr1p has significant roles within ribosome biogenesis and nucleolar pathways. It interacts closely with enzymes like RNA polymerase I involved in rRNA transcription and contributes to the assembly of the 40S and 60S ribosomal subunits. Nsr1p functionally relates to fibrillarin within ribosomal rRNA processing pathways as both proteins feature in the modification and cleavage of pre-rRNA needed for functional ribosomal units. These pathways ensure the efficient production and maturation of ribosomes necessary for protein translation.

Disruptions in Nsr1p expression or mutation can link to conditions like ribosomopathies and potential cancer development. Alterations in its function might lead to improper ribosome assembly and reduced cellular proliferative capacity seen in specific anemias or developmental disorders associated with defective ribosome production. In certain cancers aberrations in nucleolar functions involving rRNA processing hint at possible disruptions in proteins like UBF interacting with Nsr1p-related pathways potentially leading to uncontrolled cellular growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in pre-rRNA processing (PubMed : 1644811). Specifically binds nuclear localization sequences (PubMed : 1706724). Candidate for a receptor at the nucleus that may be involved in both RNA and protein transport (Probable). Binds telomeric sequences of the type (TG[1-3])n in vitro (PubMed : 7800479).
See full target information NSR1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 84:1811-1815.e3 PubMed38701742

2024

On the covalent nature of lysine polyphosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Azevedo,Filipy Borghi,Xue Bessie Su,Adolfo Saiardi

Nucleic acids research 50:11799-11819 PubMed36350639

2022

Type I arginine methyltransferases are intervention points to unveil the oncogenic Epstein-Barr virus to the immune system.

Applications

Unspecified application

Species

Unspecified reactive species

Gaelle Angrand,Alicia Quillévéré,Nadège Loaëc,Van-Trang Dinh,Ronan Le Sénéchal,Rahima Chennoufi,Patricia Duchambon,Marc Keruzoré,Rodrigo Prado Martins,Marie-Paule Teulade-Fichou,Robin Fåhraeus,Marc Blondel

The Journal of biological chemistry 295:1439-1451 PubMed31844018

2019

Development of a yeast model to study the contribution of vacuolar polyphosphate metabolism to lysine polyphosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Azevedo,Yann Desfougères,Yannasittha Jiramongkol,Hamish Partington,Sasanan Trakansuebkul,Jyoti Singh,Nicole Steck,Henning J Jessen,Adolfo Saiardi

Nature communications 8:16043 PubMed28685753

2017

Nucleolin directly mediates Epstein-Barr virus immune evasion through binding to G-quadruplexes of EBNA1 mRNA.

Applications

WB

Species

Unspecified reactive species

María José Lista,Rodrigo Prado Martins,Olivier Billant,Marie-Astrid Contesse,Sarah Findakly,Pierre Pochard,Chrysoula Daskalogianni,Claire Beauvineau,Corinne Guetta,Christophe Jamin,Marie-Paule Teulade-Fichou,Robin Fåhraeus,Cécile Voisset,Marc Blondel
View all publications
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