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AB272624

Anti-NSUN2/SAKI antibody

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(1 Publication)

Rabbit Polyclonal NSUN2/SAKI antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NSUN2 aa 500 to C-terminus.

View Alternative Names

SAKI, TRM4, NSUN2, RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, mRNA cytosine C(5)-methyltransferase, tRNA cytosine C(5)-methyltransferase, tRNA methyltransferase 4 homolog, Misu, hTrm4

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN2/SAKI antibody (AB272624)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN2/SAKI antibody (AB272624)

Paraffin-embedded formalin-fixed Human testis tissue stained for NSUN2/SAKI using ab272624 at 1/1000 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN2/SAKI antibody (AB272624)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN2/SAKI antibody (AB272624)

Paraffin-embedded formalin-fixed Human skeletal tissue stained for NSUN2/SAKI using ab272624 at 1/1000 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN2/SAKI antibody (AB272624)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN2/SAKI antibody (AB272624)

Paraffin-embedded formalin-fixed Human duodenum tissue stained for NSUN2/SAKI using ab272624 at 1/1000 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN2/SAKI antibody (AB272624)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN2/SAKI antibody (AB272624)

Paraffin-embedded formalin-fixed Human skin tissue stained for NSUN2/SAKI using ab272624 at 1/1000 in immunohistochemical analysis.

Western blot - Anti-NSUN2/SAKI antibody (AB272624)
  • WB

Unknown

Western blot - Anti-NSUN2/SAKI antibody (AB272624)

Lanes 1 and 3:

Western blot - Anti-NSUN2/SAKI antibody (ab272624) at 0.4 µg/mL

Lane 2:

Western blot - Anti-NSUN2/SAKI antibody (ab272624)

Lane 1:

U-2 OS cells transfected with target siRNA 1

Lane 2:

U-2 OS cells transfected with target siRNA 2

Lane 3:

U-2 OS cells transfected with control siRNA

Predicted band size: 86 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NSUN2 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q08J23

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSUN2 also known as SAKI is a methyltransferase enzyme that adds a methyl group to cytosine within nucleic acids most often in RNA. It has a molecular mass of about 83 kDa. This protein is widely expressed in different tissues but shows high expression levels in the brain and reproductive organs. NSUN2 modifies RNA molecules impacting their function and stability which is essential in various cellular processes.
Biological function summary

NSUN2 participates in RNA metabolism and processing. By introducing m5C (5-methylcytosine) modifications to RNA NSUN2 influences RNA stability transport and translation. This protein is a part of larger complexes that regulate diverse cellular functions and maintains transcriptome integrity. Alterations in NSUN2 activity can affect cellular proliferation and differentiation highlighting its role in maintaining normal cell function.

Pathways

NSUN2 plays an integral role in the epitranscriptomic regulation pathway in particular RNA modification and gene expression regulation pathways. It acts alongside proteins like TRMT2A and TRMT61A which are involved in similar processes of RNA methylation and modification. These pathways help maintain a balance in gene expression profiles necessary for proper cellular function and response to external signals.

Mutations or dysfunctions in NSUN2 are associated with mental retardation and cancer. In neurodevelopmental disorders altered NSUN2 activity disrupts normal brain development potentially linking it with proteins like FTSJ1 another RNA methyltransferase. Similarly in cancer NSUN2 dysregulation leads to aberrant cell proliferation and tumorigenesis often in connection with oncogenic pathways and other modified proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA cytosine C(5)-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs) (PubMed : 17071714, PubMed : 22995836, PubMed : 31199786, PubMed : 31358969). Involved in various processes, such as epidermal stem cell differentiation, testis differentiation and maternal to zygotic transition during early development : acts by increasing protein synthesis; cytosine C(5)-methylation promoting tRNA stability and preventing mRNA decay (PubMed : 31199786). Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors (PubMed : 17071714, PubMed : 22995836, PubMed : 31199786). tRNA methylation is required generation of RNA fragments derived from tRNAs (tRFs) (PubMed : 31199786). Also mediates C(5)-methylation of mitochondrial tRNAs (PubMed : 31276587). Catalyzes cytosine C(5)-methylation of mRNAs, leading to stabilize them and prevent mRNA decay : mRNA stabilization involves YBX1 that specifically recognizes and binds m5C-modified transcripts (PubMed : 22395603, PubMed : 31358969, PubMed : 34556860). Cytosine C(5)-methylation of mRNAs also regulates mRNA export : methylated transcripts are specifically recognized by THOC4/ALYREF, which mediates mRNA nucleo-cytoplasmic shuttling (PubMed : 28418038). Also mediates cytosine C(5)-methylation of non-coding RNAs, such as vault RNAs (vtRNAs), promoting their processing into regulatory small RNAs (PubMed : 23871666). Cytosine C(5)-methylation of vtRNA VTRNA1.1 promotes its processing into small-vault RNA4 (svRNA4) and regulates epidermal differentiation (PubMed : 31186410). May act downstream of Myc to regulate epidermal cell growth and proliferation (By similarity). Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity (PubMed : 19596847).
See full target information NSUN2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 39:6906-6919 PubMed32978516

2020

Aberrant NSUN2-mediated mC modification of H19 lncRNA is associated with poor differentiation of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Sun,Songlei Xue,Meiying Zhang,Hui Xu,Xuming Hu,Shihao Chen,Yangyang Liu,Mingzhou Guo,Hengmi Cui
View all publications
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Product promise

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