JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB272616

Anti-NSUN3 antibody

Be the first to review this product! Submit a review

|

(5 Publications)

Rabbit Polyclonal NSUN3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NSUN3 aa 100-250.

View Alternative Names

MSTP077, UG0651E06, NSUN3, NOL1/NOP2/Sun domain family member 3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN3 antibody (AB272616)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN3 antibody (AB272616)

Paraffin-embedded formalin-fixed Human urinary bladder tissue stained for NSUN3 using ab272616 at 1/200 in immunohistochemical analysis.

Western blot - Anti-NSUN3 antibody (AB272616)
  • WB

Unknown

Western blot - Anti-NSUN3 antibody (AB272616)

All lanes:

Western blot - Anti-NSUN3 antibody (ab272616) at 0.4 µg/mL

Lane 1:

Control (vector only transfected HEK-293T lysate)

Lane 2:

NSUN3 over-expression lysate (mammalian HEK-293T cells)

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NSUN3 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q9H649

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NSUN3 protein also known as NOP2/Sun domain family member 3 functions as an RNA methyltransferase. It specifically catalyzes the methylation of cytosine in mitochondrial tRNA molecules which is a critical step for proper mitochondrial protein translation. NSUN3 is approximately 60 kDa in mass. It expresses mostly in tissues with high energy demands such as heart and muscle tissues due to its role in mitochondrial function.
Biological function summary

The function of NSUN3 is tightly linked to mitochondrial ribosome structure and translation. By methylating tRNA at cytosine-34 NSUN3 participates in the formation of the anticodon wobble position which is vital for accurate and efficient mitochondrial protein synthesis. It does not act alone as it often functions as part of larger mitochondrial translation complexes contributing to proper mitochondrial ribosomal assembly and function.

Pathways

NSUN3 is an integral component of the mitochondrial gene expression and oxidative phosphorylation pathways. It plays a significant role in regulating mitochondrial translation by directly modifying tRNA and interacts with other proteins like TRMT10C that also participate in this modification process. These pathways are important for maintaining mitochondrial energy metabolism and production of ATP within cells.

NSUN3 deficiency associates with mitochondrial disorders that manifest as systemic energy production failures due to impaired mitochondrial translation. These deficiencies often overlap with conditions like mitochondrial myopathy or lactic acidosis. Additionally the malfunction or absence of NSUN3 can disrupt interactions with other mitochondrial proteins like MTO1 further exacerbating mitochondrial dysfunction and leading to disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C) at position 34 of mt-tRNA(Met) (PubMed : 27214402, PubMed : 27356879, PubMed : 27497299). mt-tRNA(Met) methylation at cytosine(34) takes place at the wobble position of the anticodon and initiates the formation of 5-formylcytosine (f(5)c) at this position (PubMed : 27214402, PubMed : 27356879, PubMed : 27497299). mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation (PubMed : 27356879, PubMed : 27497299).
See full target information NSUN3
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 80:100662 PubMed40279954

2025

Inhibition of NSUN3 suppresses immune escape in non-small cell lung cancer through stabilizing PD-L1 in a 5-methyladenosine dependent way.

Applications

Unspecified application

Species

Unspecified reactive species

Hancong Huang,Xiaohong Lv,Qianhua Chen,Lixia Dong

Clinical and experimental pharmacology & physiology 52:e70026 PubMed39924309

2025

NSUN3 Aggravates Sepsis-Associated Acute Kidney Injury by Stabilising TIFA mRNA Through m5C.

Applications

Unspecified application

Species

Unspecified reactive species

Chenran Zhang,Wenming Shen,Xuwen Zheng,Ming Zhu,Kaiqi Xu,Hai Huang,Jinnan Yin

American journal of cancer research 14:1768-1783 PubMed38726285

2024

Roles of RNA mC modification patterns in prognosis and tumor microenvironment infiltration of diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Cui,Cancan Luo,Lili Zhou,Tiantian Yu,Yongsheng Meng,Qianqian Yu,Zhixiang Lei,Ya Wang,Lijuan Peng,Qingqing Luo,Duozhuang Tang,Ruifang Sun,Li Yu

Cancer gene therapy 30:149-162 PubMed36123390

2022

YAP 5-methylcytosine modification increases its mRNA stability and promotes the transcription of exosome secretion-related genes in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Yu,Congcong Zhang,Yikun Wang,Xiaoting Tian,Yayou Miao,Fanyu Meng,Lifang Ma,Xiao Zhang,Jinjing Xia

Oral diseases 30:313-328 PubMed35997137

2022

RNA 5-Methylcytosine Regulator NSUN3 promotes tumor progression through regulating immune infiltration in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shufang Jin,Jiayi Li,Yihan Shen,Yiqun Wu,Zhiyuan Zhang,Hailong Ma
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB235430

Anti-NSUN4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN4 antibody (AB235430)

  • 0
  • 0
Primary Antibodies

AB121633

Anti-NSUN5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NSUN5 antibody (AB121633)

  • 4
  • 1
Primary Antibodies

AB307430

Anti-NSUN6 antibody [EPR27179-64]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-NSUN6 antibody [EPR27179-64] (AB307430)

  • 0
  • 0
Primary Antibodies

AB220175

Anti-Dnmt2 antibody [ABM1H70]

Flow Cytometry - Anti-Dnmt2 antibody [ABM1H70] (AB220175)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com