Rabbit Polyclonal NSUN4 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NSUN4 aa 1-250.
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- PLoS biology 17:e30002972019Cytosine-5 RNA methylation links protein synthesis to cell metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNikoletta A Gkatza et. al.PubMed 31199786
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NSUN4 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q96CB9
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4.
Alternative names
5-methylcytosine rRNA methyltransferase NSUN4, 5-methylcytosine tRNA methyltransferase NSUN4, NOL1/NOP2/Sun domain family member 4, NSUN4
Recommended products
Rabbit Polyclonal NSUN4 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NSUN4 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NSUN4 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q96CB9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NSUN4 also known as NOP2/Sun domain family member 4 is a methyltransferase enzyme that modifies RNA molecules by adding a methyl group to cytosine residues. It has a mass of approximately 55 kDa. NSUN4 is found in the mitochondria where it is expressed in various tissues especially in those with high energy demands such as muscle and nerve tissues. Its function is essential for mitochondrial ribosomal activity contributing to the proper synthesis of proteins within the mitochondrion.
- Biological function summary
NSUN4 plays a role in mitochondrial translation by forming a complex with MTERF4. This complex is important for the methylation of mitochondrial rRNA which ensures the correct assembly and functionality of the mitochondrial ribosome. Through this modification NSUN4 influences the expression of the mitochondrial genome supporting efficient protein synthesis necessary for cellular energetics and function.
- Pathways
NSUN4 integrates into mitochondrial biogenesis and gene expression pathways. It is significantly involved in the regulation of mitochondrial translation a subprocess of the broader mitochondrial translation pathway. NSUN4's activity closely interacts with proteins such as MTERF4 which assists in maintaining mitochondrial DNA transcription and replication illustrating its critical place in sustaining mitochondrial integrity and energy homeostasis.
- Associated diseases and disorders
NSUN4 has implications in mitochondrial-related disorders particularly those affecting energy-dependent tissues. Dysfunction in NSUN4 can lead to challenges in mitochondrial bioenergetics contributing to conditions like mitochondrial myopathy and neurodegenerative diseases which can result from impaired mitochondrial translation. Additionally its interplay with MTERF4 suggests that disruptions in this protein complex might exacerbate conditions related to mitochondrial gene expression defects.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-NSUN4 antibody (ab101625)
10% SDS PAGE
All lanes: Western blot - Anti-NSUN4 antibody (ab101625) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 43 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-NSUN4 antibody (ab101625)
NSUN4 western blot using anti-NSUN4 antibody ab101625. Publication image and figure legend from Gkatza, N. A., Castro, C., et al., 2019, PLoS Biol, PubMed 31199786.
ab101625 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab101625 please see the product overview.
Levels of m5C changes site-specifically and dynamically in response to oxidative stress.(A) Time course of sodium arsenite treatment. (B) Log2 FC of Nsun2 RNA expression in NSUN2+/+ and NSUN2+/− cells relative to GAPDH and normalised to the untreated control (‘Ctr’). Shown are 3 replicates. (C) Western blot analysis of the indicated proteins using whole cell lysates from NSUN2+/+ and NSUN2−/− cells. Hsp90 served as a loading control. (D,E) Detection of m5C in sodium arsenite–treated and untreated (‘ctr’) NSUN2+/+ and −/− cells using mass spectrometry. (n = 3 samples per time point). (F) Quantification of tRNA methylation percentage using RNA bisulfite sequencing of NSUN2+/+ and NSUN2−/− cells (n = 4 samples per time point). (G) Heatmap of methylation status of individual tRNA molecules shown in (F). (H,I) Quantification (H) and heatmap (I) of methylation changes in the tRNAs LeuCAA and AspGTC in NSUN2+/+ and NSUN2−/− cells. (J) Quantification of methylation in non-tRNA targets. Data represent median in F, H, and J. Error bars are ±SD. p-Values: Student’s t test, *p < 0.05 and **p < 0.01. ***p < 0.001. The underlying data for this figure can be found in S8 and S9 Data and S1 File. FC, fold-change; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HSP90, heat shock protein 90; m5C, 5-methylcytosine; NPMI, nucleophosmin; tRNA, transfer RNA; VL, variable loop.
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