Anti-NSUN4 antibody

• WB

Unknown

Western blot - Anti-NSUN4 antibody (AB101625)

10% SDS PAGE

All lanes:

Western blot - Anti-NSUN4 antibody (ab101625) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 43 kDa

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• WB

Supplier Data

Western blot - Anti-NSUN4 antibody (AB101625)

Samples were separated by 10% SDS PAGE. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-NSUN4 antibody (ab101625) at 1/1000 dilution

Lane 1:

Mouse muscle tissue extracts at 50 µg

Lane 2:

Mouse eye tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 43 kDa

true

• WB

Supplier Data

Western blot - Anti-NSUN4 antibody (AB101625)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-NSUN4 antibody (ab101625) at 1/500 dilution

All lanes:

Mouse liver tissue extract at 50 µg

Predicted band size: 43 kDa

false

• WB

CiteAb

Western blot - Anti-NSUN4 antibody (AB101625)

NSUN4 western blot using anti-NSUN4 antibody ab101625. Publication image and figure legend from Gkatza, N. A., Castro, C., et al., 2019, PLoS Biol, PubMed 31199786.

ab101625 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab101625 please see the product overview.

Levels of m5C changes site-specifically and dynamically in response to oxidative stress.(A) Time course of sodium arsenite treatment. (B) Log2 FC of Nsun2 RNA expression in NSUN2+/+ and NSUN2+/- cells relative to GAPDH and normalised to the untreated control ('Ctr'). Shown are 3 replicates. (C) Western blot analysis of the indicated proteins using whole cell lysates from NSUN2+/+ and NSUN2-/- cells. Hsp90 served as a loading control. (D,E) Detection of m5C in sodium arsenite–treated and untreated ('ctr') NSUN2+/+ and -/- cells using mass spectrometry. (n = 3 samples per time point). (F) Quantification of tRNA methylation percentage using RNA bisulfite sequencing of NSUN2+/+ and NSUN2-/- cells (n = 4 samples per time point). (G) Heatmap of methylation status of individual tRNA molecules shown in (F). (H,I) Quantification (H) and heatmap (I) of methylation changes in the tRNAs LeuCAA and AspGTC in NSUN2+/+ and NSUN2-/- cells. (J) Quantification of methylation in non-tRNA targets. Data represent median in F, H, and J. Error bars are ±SD. p-Values : Student's t test, *p < 0.05 and **p < 0.01. ***p < 0.001. The underlying data for this figure can be found in S8 and S9 Data and S1 File. FC, fold-change; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HSP90, heat shock protein 90; m5C, 5-methylcytosine; NPMI, nucleophosmin; tRNA, transfer RNA; VL, variable loop.

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