JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB235430

Anti-NSUN4 antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal NSUN4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NSUN4 aa 1-300.

View Alternative Names

5-cytosine rRNA methyltransferase NSUN4, 5-cytosine tRNA methyltransferase NSUN4, NOL1/NOP2/Sun domain family member 4, mRNA cytosine C(5)-methyltransferase NSUN4, NSUN4

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN4 antibody (AB235430)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN4 antibody (AB235430)

Paraffin-embedded human colon cancer tissue stained for NSUN4 with ab235430 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-NSUN4 antibody (AB235430)
  • WB

Supplier Data

Western blot - Anti-NSUN4 antibody (AB235430)

All lanes:

Western blot - Anti-NSUN4 antibody (ab235430) at 1/500 dilution

All lanes:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NSUN4 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q96CB9

style
left-column

Alternative Products

Primary Antibodies

AB101625

Anti-NSUN4 antibody

Western blot - Anti-NSUN4 antibody (AB101625)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB272616

Anti-NSUN3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN3 antibody (AB272616)

  • 0
  • 0
Primary Antibodies

AB121633

Anti-NSUN5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NSUN5 antibody (AB121633)

  • 4
  • 1
Primary Antibodies

AB307430

Anti-NSUN6 antibody [EPR27179-64]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-NSUN6 antibody [EPR27179-64] (AB307430)

  • 0
  • 0
Primary Antibodies

AB259941

Anti-NSUN2/SAKI antibody [EPR24140-93]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-NSUN2/SAKI antibody [EPR24140-93] (AB259941)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSUN4 also known as NOP2/Sun domain family member 4 is a methyltransferase enzyme that modifies RNA molecules by adding a methyl group to cytosine residues. It has a mass of approximately 55 kDa. NSUN4 is found in the mitochondria where it is expressed in various tissues especially in those with high energy demands such as muscle and nerve tissues. Its function is essential for mitochondrial ribosomal activity contributing to the proper synthesis of proteins within the mitochondrion.
Biological function summary

NSUN4 plays a role in mitochondrial translation by forming a complex with MTERF4. This complex is important for the methylation of mitochondrial rRNA which ensures the correct assembly and functionality of the mitochondrial ribosome. Through this modification NSUN4 influences the expression of the mitochondrial genome supporting efficient protein synthesis necessary for cellular energetics and function.

Pathways

NSUN4 integrates into mitochondrial biogenesis and gene expression pathways. It is significantly involved in the regulation of mitochondrial translation a subprocess of the broader mitochondrial translation pathway. NSUN4's activity closely interacts with proteins such as MTERF4 which assists in maintaining mitochondrial DNA transcription and replication illustrating its critical place in sustaining mitochondrial integrity and energy homeostasis.

NSUN4 has implications in mitochondrial-related disorders particularly those affecting energy-dependent tissues. Dysfunction in NSUN4 can lead to challenges in mitochondrial bioenergetics contributing to conditions like mitochondrial myopathy and neurodegenerative diseases which can result from impaired mitochondrial translation. Additionally its interplay with MTERF4 suggests that disruptions in this protein complex might exacerbate conditions related to mitochondrial gene expression defects.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Mitochondrial RNA cytosine C(5)-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as rRNAs, mRNAs and some long non-coding RNAs (lncRNAs) (PubMed : 21531335, PubMed : 23022348, PubMed : 39019044). Involved in mitochondrial ribosome small subunit (SSU) maturation by catalyzing methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU) (PubMed : 21531335, PubMed : 23022348). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled (PubMed : 21531335, PubMed : 23022348). In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4 (PubMed : 23022348). Also acts as a regulator of innate immunity by marking double-stranded mitochondrial RNAs(mt-dsRNAs) generated in response to stress : catalyzes m5C modification on mitochondrial RNAs, such as a mRNAs and lncRNAs, with a preference for the termini of light-strand lncRNAs, promoting their degradation and cytosolic release (PubMed : 39019044). Modified light-strand lncRNAs are then recognized by C1QBP reader and recruited to the mitochondrial degradosome complex, which promotes their degradation (PubMed : 39019044).
See full target information NSUN4
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 14:1768-1783 PubMed38726285

2024

Roles of RNA mC modification patterns in prognosis and tumor microenvironment infiltration of diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Cui,Cancan Luo,Lili Zhou,Tiantian Yu,Yongsheng Meng,Qianqian Yu,Zhixiang Lei,Ya Wang,Lijuan Peng,Qingqing Luo,Duozhuang Tang,Ruifang Sun,Li Yu

Molecules and cells 46:351-359 PubMed36921992

2023

RNA Editing Enzyme ADAR1 Suppresses the Mobility of Cancer Cells via ARPIN.

Applications

Unspecified application

Species

Unspecified reactive species

Min Ji Park,Eunji Jeong,Eun Ji Lee,Hyeon Ji Choi,Bo Hyun Moon,Keunsoo Kang,Suhwan Chang

Cancer gene therapy 30:149-162 PubMed36123390

2022

YAP 5-methylcytosine modification increases its mRNA stability and promotes the transcription of exosome secretion-related genes in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Yu,Congcong Zhang,Yikun Wang,Xiaoting Tian,Yayou Miao,Fanyu Meng,Lifang Ma,Xiao Zhang,Jinjing Xia
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com