Rabbit Polyclonal NSUN4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NSUN4 aa 1-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NSUN4 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96CB9
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
Associated Products
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Target data
Function
Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4.
Alternative names
5-methylcytosine rRNA methyltransferase NSUN4, 5-methylcytosine tRNA methyltransferase NSUN4, NOL1/NOP2/Sun domain family member 4, NSUN4
Recommended products
Rabbit Polyclonal NSUN4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NSUN4 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NSUN4 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96CB9
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NSUN4 also known as NOP2/Sun domain family member 4 is a methyltransferase enzyme that modifies RNA molecules by adding a methyl group to cytosine residues. It has a mass of approximately 55 kDa. NSUN4 is found in the mitochondria where it is expressed in various tissues especially in those with high energy demands such as muscle and nerve tissues. Its function is essential for mitochondrial ribosomal activity contributing to the proper synthesis of proteins within the mitochondrion.
- Biological function summary
NSUN4 plays a role in mitochondrial translation by forming a complex with MTERF4. This complex is important for the methylation of mitochondrial rRNA which ensures the correct assembly and functionality of the mitochondrial ribosome. Through this modification NSUN4 influences the expression of the mitochondrial genome supporting efficient protein synthesis necessary for cellular energetics and function.
- Pathways
NSUN4 integrates into mitochondrial biogenesis and gene expression pathways. It is significantly involved in the regulation of mitochondrial translation a subprocess of the broader mitochondrial translation pathway. NSUN4's activity closely interacts with proteins such as MTERF4 which assists in maintaining mitochondrial DNA transcription and replication illustrating its critical place in sustaining mitochondrial integrity and energy homeostasis.
- Associated diseases and disorders
NSUN4 has implications in mitochondrial-related disorders particularly those affecting energy-dependent tissues. Dysfunction in NSUN4 can lead to challenges in mitochondrial bioenergetics contributing to conditions like mitochondrial myopathy and neurodegenerative diseases which can result from impaired mitochondrial translation. Additionally its interplay with MTERF4 suggests that disruptions in this protein complex might exacerbate conditions related to mitochondrial gene expression defects.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-NSUN4 antibody (ab235430)
All lanes: Western blot - Anti-NSUN4 antibody (ab235430) at 1/500 dilution
All lanes: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN4 antibody (ab235430)
Paraffin-embedded human colon cancer tissue stained for NSUN4 with ab235430 at 1/100 dilution in immunohistochemical analysis.
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