Rabbit Polyclonal NSUN5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NSUN5 aa 100-250.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 3782 (m5C3782) in 28S rRNA (PubMed:23913415, PubMed:31428936, PubMed:31722427). m5C3782 promotes protein translation without affecting ribosome biogenesis and fidelity (PubMed:31428936, PubMed:31722427). Required for corpus callosum and cerebral cortex development (By similarity).
NSUN5A, WBSCR20, WBSCR20A, NSUN5, 28S rRNA (cytosine-C(5))-methyltransferase, NOL1-related protein, NOL1/NOP2/Sun domain family member 5, Williams-Beuren syndrome chromosomal region 20A protein, NOL1R
Rabbit Polyclonal NSUN5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NSUN5 aa 100-250.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
NSUN5 also known as NOL1/NOP2/Sun domain family member 5 is a methyltransferase enzyme responsible for the methylation of cytosine residues in RNA. It has an estimated mass of approximately 50 kDa. Researchers have found NSUN5 expressed in various tissues with significant levels observed in the liver brain and reproductive tissues. Its primary mechanical function facilitates the post-transcriptional modification of RNA influencing RNA stability and translation efficiency.
The methylation activity of NSUN5 contributes to cellular processes like RNA processing and gene expression regulation. NSUN5 forms part of the sub-complex associated with the large ribosomal subunit in eukaryotic cells. This connection suggests its role in ribosome biogenesis and protein synthesis. Studies also highlight NSUN5's involvement in safeguarding cell survival under stress by maintaining ribosomal RNA (rRNA) stability.
NSUN5 operates as an integral component in the ribosome biogenesis pathway and the RNA modification pathway. It interacts with other proteins such as NSUN2 and NSUN6 which share similar RNA methylation functions. These interactions suggest a cooperative mechanism among NSUN family members in ensuring proper RNA and ribosome functionality important under both normal and stress conditions.
Researchers have linked NSUN5 to several health conditions including cancer and aging-related diseases. Elevated NSUN5 expression has been observed in various cancers proposing its role in tumorigenesis through dysregulation of cell growth control. Additionally its association with shortened human lifespan suggests an involvement in age-related changes through disrupted rRNA methylation. NSUN5's interaction with NSUN2 supports a complex network where alterations in RNA methylation contribute to disease development and progression.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and nucleoli. Recommended concentration of ab121633 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes: Western blot - Anti-NSUN5 antibody (ab121633) at 1/250 dilution
Lane 1: RT4 cell lysate
Lane 2: U-251 MG cell lysate
Lane 3: Human Plasma lysate
Lane 4: Human Liver lysate
Lane 5: Human Tonsil lysate
Developed using the ECL technique.
Predicted band size: 47 kDa
ab121633 at 1/250 dilution staining NSUN5 in Paraffin-embedded Human rectum tissue by Immunohistochemistry.
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