JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB121633

Anti-NSUN5 antibody

4

(1 Review)

|

(4 Publications)

Rabbit Polyclonal NSUN5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NSUN5 aa 100-250.

View Alternative Names

NSUN5A, WBSCR20, WBSCR20A, NSUN5, 28S rRNA (cytosine-C(5))-methyltransferase, NOL1-related protein, NOL1/NOP2/Sun domain family member 5, Williams-Beuren syndrome chromosomal region 20A protein, NOL1R

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-NSUN5 antibody (AB121633)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NSUN5 antibody (AB121633)

Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and nucleoli. Recommended concentration of ab121633 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN5 antibody (AB121633)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN5 antibody (AB121633)

ab121633 at 1/250 dilution staining NSUN5 in Paraffin-embedded Human rectum tissue by Immunohistochemistry.

Western blot - Anti-NSUN5 antibody (AB121633)
  • WB

Unknown

Western blot - Anti-NSUN5 antibody (AB121633)

All lanes:

Western blot - Anti-NSUN5 antibody (ab121633) at 1/250 dilution

Lane 1:

RT4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human Plasma lysate

Lane 4:

Human Liver lysate

Lane 5:

Human Tonsil lysate

Predicted band size: 47 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NSUN5 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q96P11

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSUN5 also known as NOL1/NOP2/Sun domain family member 5 is a methyltransferase enzyme responsible for the methylation of cytosine residues in RNA. It has an estimated mass of approximately 50 kDa. Researchers have found NSUN5 expressed in various tissues with significant levels observed in the liver brain and reproductive tissues. Its primary mechanical function facilitates the post-transcriptional modification of RNA influencing RNA stability and translation efficiency.
Biological function summary

The methylation activity of NSUN5 contributes to cellular processes like RNA processing and gene expression regulation. NSUN5 forms part of the sub-complex associated with the large ribosomal subunit in eukaryotic cells. This connection suggests its role in ribosome biogenesis and protein synthesis. Studies also highlight NSUN5's involvement in safeguarding cell survival under stress by maintaining ribosomal RNA (rRNA) stability.

Pathways

NSUN5 operates as an integral component in the ribosome biogenesis pathway and the RNA modification pathway. It interacts with other proteins such as NSUN2 and NSUN6 which share similar RNA methylation functions. These interactions suggest a cooperative mechanism among NSUN family members in ensuring proper RNA and ribosome functionality important under both normal and stress conditions.

Researchers have linked NSUN5 to several health conditions including cancer and aging-related diseases. Elevated NSUN5 expression has been observed in various cancers proposing its role in tumorigenesis through dysregulation of cell growth control. Additionally its association with shortened human lifespan suggests an involvement in age-related changes through disrupted rRNA methylation. NSUN5's interaction with NSUN2 supports a complex network where alterations in RNA methylation contribute to disease development and progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 3782 (m5C3782) in 28S rRNA (PubMed : 23913415, PubMed : 31428936, PubMed : 31722427). m5C3782 promotes protein translation without affecting ribosome biogenesis and fidelity (PubMed : 31428936, PubMed : 31722427). Required for corpus callosum and cerebral cortex development (By similarity).
See full target information NSUN5
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:907 PubMed40804410

2025

DNA methylation variations of DNA damage response in glioblastoma: NSUN5 modulates tumor-intrinsic cytosolic DNA-sensing and microglial behavior.

Applications

Unspecified application

Species

Unspecified reactive species

An-An Yin,Yan Yao,Yi-Feng Liu,Yu-Sha Ji,Ya-Long He,Tian-Chi Ma,Wen-Heng Guo,Amandine Etcheverry,Marc Aubry,Jean Mosser,Wei Lin,Yuan-Ming Wu,Kun Chen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2404083 PubMed39531371

2024

NSUN5 Facilitates Hepatocellular Carcinoma Progression by Increasing SMAD3 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hexu Han,Chengcheng Zhang,Wenbo Shi,Jiawei Wang,Wenhui Zhao,Yanping Du,Zhibin Zhao,Yifan Wang,Maosong Lin,Lei Qin,Xiaoxue Zhao,Qianqian Yin,Yiyi Liu,Zhongqi Wang,Jing Zhang,Xiaomin You,Guoxiong Zhou,Honghui Wu,Jun Ye,Xianzhong He,Weizhong Tian,Hong Yu,Yin Yuan,Qiang Wang

Life science alliance 6: PubMed36446521

2022

Loss of NDR1/2 kinases impairs endomembrane trafficking and autophagy leading to neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Flavia Roşianu,Simeon R Mihaylov,Noreen Eder,Antonie Martiniuc,Suzanne Claxton,Helen R Flynn,Shamsinar Jalal,Marie-Charlotte Domart,Lucy Collinson,Mark Skehel,Ambrosius P Snijders,Matthias Krause,Sharon A Tooze,Sila K Ultanir

Cancer gene therapy 30:149-162 PubMed36123390

2022

YAP 5-methylcytosine modification increases its mRNA stability and promotes the transcription of exosome secretion-related genes in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Yu,Congcong Zhang,Yikun Wang,Xiaoting Tian,Yayou Miao,Fanyu Meng,Lifang Ma,Xiao Zhang,Jinjing Xia
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB235430

Anti-NSUN4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN4 antibody (AB235430)

  • 0
  • 0
Primary Antibodies

AB272616

Anti-NSUN3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN3 antibody (AB272616)

  • 0
  • 0
Primary Antibodies

AB220175

Anti-Dnmt2 antibody [ABM1H70]

Flow Cytometry - Anti-Dnmt2 antibody [ABM1H70] (AB220175)

  • 0
  • 0
Primary Antibodies

AB259941

Anti-NSUN2/SAKI antibody [EPR24140-93]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-NSUN2/SAKI antibody [EPR24140-93] (AB259941)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com