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Rabbit Polyclonal NSUN5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NSUN5 aa 100-250.


Images

Immunocytochemistry/ Immunofluorescence - Anti-NSUN5 antibody (AB121633), expandable thumbnail
  • Western blot - Anti-NSUN5 antibody (AB121633), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN5 antibody (AB121633), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human NSUN5 aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q96P11

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

-

Target data

Function

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 3782 (m5C3782) in 28S rRNA (PubMed:23913415, PubMed:31428936, PubMed:31722427). m5C3782 promotes protein translation without affecting ribosome biogenesis and fidelity (PubMed:31428936, PubMed:31722427). Required for corpus callosum and cerebral cortex development (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal NSUN5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NSUN5 aa 100-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human NSUN5 aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q96P11
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NSUN5 also known as NOL1/NOP2/Sun domain family member 5 is a methyltransferase enzyme responsible for the methylation of cytosine residues in RNA. It has an estimated mass of approximately 50 kDa. Researchers have found NSUN5 expressed in various tissues with significant levels observed in the liver brain and reproductive tissues. Its primary mechanical function facilitates the post-transcriptional modification of RNA influencing RNA stability and translation efficiency.

Biological function summary

The methylation activity of NSUN5 contributes to cellular processes like RNA processing and gene expression regulation. NSUN5 forms part of the sub-complex associated with the large ribosomal subunit in eukaryotic cells. This connection suggests its role in ribosome biogenesis and protein synthesis. Studies also highlight NSUN5's involvement in safeguarding cell survival under stress by maintaining ribosomal RNA (rRNA) stability.

Pathways

NSUN5 operates as an integral component in the ribosome biogenesis pathway and the RNA modification pathway. It interacts with other proteins such as NSUN2 and NSUN6 which share similar RNA methylation functions. These interactions suggest a cooperative mechanism among NSUN family members in ensuring proper RNA and ribosome functionality important under both normal and stress conditions.

Associated diseases and disorders

Researchers have linked NSUN5 to several health conditions including cancer and aging-related diseases. Elevated NSUN5 expression has been observed in various cancers proposing its role in tumorigenesis through dysregulation of cell growth control. Additionally its association with shortened human lifespan suggests an involvement in age-related changes through disrupted rRNA methylation. NSUN5's interaction with NSUN2 supports a complex network where alterations in RNA methylation contribute to disease development and progression.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-NSUN5 antibody (ab121633), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NSUN5 antibody (ab121633)

    Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and nucleoli. Recommended concentration of ab121633 1-4 µg/ml. Cells treated with PFA/Triton X-100.

  • Western blot - Anti-NSUN5 antibody (ab121633), expandable thumbnail

    Western blot - Anti-NSUN5 antibody (ab121633)

    All lanes: Western blot - Anti-NSUN5 antibody (ab121633) at 1/250 dilution

    Lane 1: RT4 cell lysate

    Lane 2: U-251 MG cell lysate

    Lane 3: Human Plasma lysate

    Lane 4: Human Liver lysate

    Lane 5: Human Tonsil lysate

    Developed using the ECL technique.

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN5 antibody (ab121633), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN5 antibody (ab121633)

    ab121633 at 1/250 dilution staining NSUN5 in Paraffin-embedded Human rectum tissue by Immunohistochemistry.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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