JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB214227

Anti-NSUN6 antibody

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Polyclonal NSUN6 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NSUN6 aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

NOPD1, NSUN6, tRNA (cytosine(72)-C(5))-methyltransferase NSUN6, NOL1/NOP2/Sun and PUA domain-containing protein 1, NOL1/NOP2/Sun domain family member 6

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN6 antibody (AB214227)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN6 antibody (AB214227)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast cancer tissue labeling NSUN6 with ab214227 at 1/500 dilution, followed by secondary antibody detection and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human NSUN6 aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8TEA1

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB235430

Anti-NSUN4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN4 antibody (AB235430)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB259941

Anti-NSUN2/SAKI antibody [EPR24140-93]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-NSUN2/SAKI antibody [EPR24140-93] (AB259941)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSUN6 also known as NOP2/Sun RNA methyltransferase family member 6 is an RNA-modifying enzyme. It belongs to the NOL1/NOP2/Sun family and is responsible for catalyzing 5-methylcytosine formation on target RNA molecules playing an important role in RNA modification. The molecular mass of NSUN6 is approximately 56 kDa. It is expressed in various tissues but shows higher expression in the liver and skeletal muscle suggesting a potential specialization in these areas.
Biological function summary

NSUN6 contributes to the regulation of cellular processes by modifying RNA molecules. It is not currently identified as part of any known protein complex but significantly influences the stability and function of specific RNA targets. By this action NSUN6 can indirectly affect gene expression and downstream biological activities which might be critical during cellular growth and differentiation.

Pathways

NSUN6 interacts closely with pathways related to RNA processing and modification. It contributes to the epitranscriptomic landscape working alongside proteins such as NSUN2 another methyltransferase to maintain RNA modification balance. This activity impacts broader pathways like those regulating gene expression and protein synthesis exhibiting an interconnected role in fundamental cellular activities.

NSUN6 shows relevance to cancer and neurodevelopmental disorders. Alterations in its normal function could disrupt RNA methylation patterns leading to aberrant gene expression linked to these conditions. NSUN6's functional relationship with NSUN2 another RNA methyltransferase suggests that disruptions in their balance might contribute to these disorders highlighting their potential as targets for therapeutic intervention.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA) (PubMed : 26160102, PubMed : 27703015, PubMed : 28531330). In vitro also methylates tRNA(Thr)(AGT) (PubMed : 26160102, PubMed : 27703015). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2 : 71 and 3 : 70) in the tRNA substrates (PubMed : 26160102, PubMed : 27703015).
See full target information NSUN6
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Medical principles and practice : international journal of the Kuwait University, Health Science Centre 33:56-65 PubMed38029727

2023

NSUN6 Regulates NM23-H1 Expression in an m5C Manner to Affect Epithelial-Mesenchymal Transition in Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhansheng Lu,Bo Liu,Demiao Kong,Xiaojiang Zhou,Dengke Pei,Di Liu

Journal of cellular and molecular medicine 27:2398-2411 PubMed37408139

2023

RNA m5C methylation orchestrates BLCA progression via macrophage reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Dali Yan,Yongsong Xie,Liyuan Huang,Yi Zhang,Runhuan Gu,Huaibing Xie,Xing Huang,Hao Luo

Cancer management and research 13:7989-8002 PubMed34707405

2021

Comprehensive Analysis of the Prognostic Value and Immune Infiltrates of the Three-m5C Signature in Colon Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qishun Geng,Qian Wei,Zhibo Shen,Yuanyuan Zheng,Longhao Wang,Wenhua Xue,Lifeng Li,Jie Zhao

Scientific reports 7:5818 PubMed28724891

2017

Hypoxia-inducible factor-1 alpha is involved in RIP-induced necroptosis caused by in vitro and in vivo ischemic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Sa Yang,Tai-Long Yi,Sai Zhang,Zhong-Wei Xu,Ze-Qi Yu,Hong-Tao Sun,Cheng Yang,Yue Tu,Shi-Xiang Cheng
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com