Rabbit Recombinant Monoclonal NSUN6 antibody. Carrier free. Suitable for IP, ICC/IF, WB and reacts with Human, Mouse, Rat samples.
Images
![Immunoprecipitation - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (AB307431), expandable thumbnail](https://content.abcam.com/products/images/nsun6-antibody-epr27179-64-bsa-and-azide-free-ab307431--immunoprecipitation-img406410.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (AB307431)")
![Western blot - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (AB307431), expandable thumbnail](https://content.abcam.com/products/images/nsun6-antibody-epr27179-64-bsa-and-azide-free-ab307431--western-blot-img405634.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (AB307431)")
![Western blot - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (AB307431), expandable thumbnail](https://content.abcam.com/products/images/nsun6-antibody-epr27179-64-bsa-and-azide-free-ab307431--western-blot-img405633.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (AB307431)")
![Immunocytochemistry/ Immunofluorescence - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (AB307431), expandable thumbnail](https://content.abcam.com/products/images/nsun6-antibody-epr27179-64-bsa-and-azide-free-ab307431--immunocytochemistry-immunofluorescence-img405631.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (AB307431)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Expected | Not recommended | Tested | Not recommended | Tested |
| Rat | Expected | Not recommended | Expected | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Target data
Function
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA) (PubMed:26160102, PubMed:27703015, PubMed:28531330). In vitro also methylates tRNA(Thr)(AGT) (PubMed:26160102, PubMed:27703015). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates (PubMed:26160102, PubMed:27703015).
Alternative names
NOPD1, NSUN6, tRNA (cytosine(72)-C(5))-methyltransferase NSUN6, NOL1/NOP2/Sun and PUA domain-containing protein 1, NOL1/NOP2/Sun domain family member 6
Recommended products
Rabbit Recombinant Monoclonal NSUN6 antibody. Carrier free. Suitable for IP, ICC/IF, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27179-64
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NSUN6 also known as NOP2/Sun RNA methyltransferase family member 6 is an RNA-modifying enzyme. It belongs to the NOL1/NOP2/Sun family and is responsible for catalyzing 5-methylcytosine formation on target RNA molecules playing an important role in RNA modification. The molecular mass of NSUN6 is approximately 56 kDa. It is expressed in various tissues but shows higher expression in the liver and skeletal muscle suggesting a potential specialization in these areas.
- Biological function summary
NSUN6 contributes to the regulation of cellular processes by modifying RNA molecules. It is not currently identified as part of any known protein complex but significantly influences the stability and function of specific RNA targets. By this action NSUN6 can indirectly affect gene expression and downstream biological activities which might be critical during cellular growth and differentiation.
- Pathways
NSUN6 interacts closely with pathways related to RNA processing and modification. It contributes to the epitranscriptomic landscape working alongside proteins such as NSUN2 another methyltransferase to maintain RNA modification balance. This activity impacts broader pathways like those regulating gene expression and protein synthesis exhibiting an interconnected role in fundamental cellular activities.
- Associated diseases and disorders
NSUN6 shows relevance to cancer and neurodevelopmental disorders. Alterations in its normal function could disrupt RNA methylation patterns leading to aberrant gene expression linked to these conditions. NSUN6's functional relationship with NSUN2 another RNA methyltransferase suggests that disruptions in their balance might contribute to these disorders highlighting their potential as targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (ab307431), expandable thumbnail](https://content.abcam.com/products/images/nsun6-antibody-epr27179-64-bsa-and-azide-free-ab307431--immunoprecipitation-img406410.jpg "Immunoprecipitation - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (ab307431)")
Immunoprecipitation - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (ab307431)
This data was developed using Anti-NSUN6 antibody [EPR27179-64] ab307430, the same antibody clone in a different buffer formulation.
NSUN6 was immunoprecipitated from 0.35 mg Hela (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 ug with Anti-NSUN6 antibody [EPR27179-64] ab307430 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-NSUN6 antibody [EPR27179-64] ab307430 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Hela (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 ug
Lane 2: ABAB307430 IP in Hela whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NSUN6 antibody [EPR27179-64] ab307430 in Hela whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 84 secondsAll lanes: Immunoprecipitation - Anti-NSUN6 antibody [EPR27179-64] (Anti-NSUN6 antibody [EPR27179-64] ab307430) at 1/1000 dilution
Lane 1: Hela (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 2: Hela whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 50 kDa
Exposure time: 84s
![Western blot - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (ab307431), expandable thumbnail](https://content.abcam.com/products/images/nsun6-antibody-epr27179-64-bsa-and-azide-free-ab307431--western-blot-img405634.jpg "Western blot - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (ab307431)")
Western blot - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (ab307431)
This data was developed using 307430, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Low expression: human skeletal muscle (PMID: 33330931).
The identity of the higher MW band at approximately 75 kDa is unknown.
Exposure time:All lanes: Western blot - Anti-NSUN6 antibody [EPR27179-64] (Anti-NSUN6 antibody [EPR27179-64] ab307430) at 1/1000 dilution
Lane 1: Human testis tissue lysate 20 μg
Lane 2: Human skeletal muscle tissue lysate 20 μg
Lane 3: Mouse brain tissue lysate 20 μg
Lane 4: Mouse embryo tissue lysate 20 μg
Lane 5: Rat testis tissue lysate 20 μg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 50 kDa
Exposure time: 180s
![Western blot - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (ab307431), expandable thumbnail](https://content.abcam.com/products/images/nsun6-antibody-epr27179-64-bsa-and-azide-free-ab307431--western-blot-img405633.jpg "Western blot - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (ab307431)")
Western blot - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (ab307431)
This data was developed using 307430, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration:
125 seconds
Exposure time:All lanes: Western blot - Anti-NSUN6 antibody [EPR27179-64] (Anti-NSUN6 antibody [EPR27179-64] ab307430) at 1/1000 dilution
Lane 1: Hela (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate 20 μg
Lane 2: Hela transfected with siRNA specifically targeti NSUN6 whole cell lysate 20 μg
Lane 3: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 20 μg
Lane 4: HepG2 (human hepatocellar carcinoma epithelial cell lysate 20 μg
Lane 5: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 μg
Lane 6: Hela (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 50 kDa
Exposure time: 125s
![Immunocytochemistry/ Immunofluorescence - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (ab307431), expandable thumbnail](https://content.abcam.com/products/images/nsun6-antibody-epr27179-64-bsa-and-azide-free-ab307431--immunocytochemistry-immunofluorescence-img405631.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (ab307431)")
Immunocytochemistry/ Immunofluorescence - Anti-NSUN6 antibody [EPR27179-64] - BSA and Azide free (ab307431)
This data was developed using Anti-NSUN6 antibody [EPR27179-64] ab307430, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized Neuro-2a (mouse neuroblastoma neuroblast) cells lABelling NSUN6 with Anti-NSUN6 antibody [EPR27179-64] ab307430 at 1/100 (4.81 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/ml dilution.
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