JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB248989

Anti-NT-4 antibody [EPR9180] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal NT-4 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

NTF5, NTF4, Neurotrophin-4, NT-4, Neurotrophin-5, Neutrophic factor 4, NT-5

1 Images
Western blot - Anti-NT-4 antibody [EPR9180] - BSA and Azide free (AB248989)
  • WB

Unknown

Western blot - Anti-NT-4 antibody [EPR9180] - BSA and Azide free (AB248989)

This data was developed using ab150437, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NT-4 antibody [EPR9180] - BSA and Azide free (ab248989) at 1/1000 dilution

Lane 1:

Human prostate cancer tissue lysate at 10 µg

Lane 2:

Human stomach tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP labelled antibody at 1/2000 dilution

Predicted band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9180

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

ab248989 is the carrier-free version of ab150437.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NT-4 also known as Neurotrophin-4 is a member of the neurotrophin family of proteins. It has a molecular mass of around 14 kDa. NT-4 is expressed in both the central and peripheral nervous systems with significant presence in the brain particularly in regions such as the cortex and hippocampus. This protein acts through binding to the TrkB receptor and plays a direct role in supporting neuronal survival and differentiation.
Biological function summary

NT-4 regulates the growth and maturation of neurons. It is a part of the neurotrophin signaling complex which includes other family members like brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). By promoting axonal growth and synaptic plasticity NT-4 influences both embryonic neurodevelopment and adult neural adaptations. Its functions are important for maintaining the health of neurons and overall brain function.

Pathways

NT-4 is involved in the neurotrophin signaling pathway and MAPK pathway. It interacts with the TrkB receptor to activate signaling cascades that regulate neuronal survival and plasticity. In these pathways proteins like PIK3CA and MAPK1 interact with NT-4 facilitating its role in signal transduction that influences neural activities and responses to external stimuli.

NT-4 is linked to neurological conditions such as Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In Alzheimer's disease NT-4 in association with BDNF influences neuronal survival and synaptic maintenance which are often compromised as the disease progresses. In ALS interactions between NT-4 and TrkB receptors may affect motor neuron health and survival therefore playing a role in disease pathology. Understanding these relationships can shed light on potential therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Target-derived survival factor for peripheral sensory sympathetic neurons (PubMed : 1742028). May promote ameloblast differentiation and subsequent reduction in proliferation of ameloblasts (By similarity).
See full target information NTF4
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 50: PubMed37732539

2023

Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhitao Chen,Peipei Li,Lingguang Shen,Xiuyu Jiang
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB16640

Anti-Sortilin/NT3 antibody

KO Validated

Western blot - Anti-Sortilin/NT3 antibody (AB16640)

  • 5
  • 10
Primary Antibodies

AB190802

Anti-Serum Amyloid A antibody [EP11592-92]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid A antibody [EP11592-92] (AB190802)

  • 0
  • 1
Primary Antibodies

AB209814

Anti-TREM2 antibody [EPR20243]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-TREM2 antibody [EPR20243] (AB209814)

  • 5
  • 5

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com