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AB13115

Anti-NT-proBNP antibody [15F11]

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(8 Publications)

Mouse Monoclonal BNP antibody. Suitable for ELISA, WB and reacts with Recombinant fragment - Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human NPPB aa 1-50.

View Alternative Names

Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, NPPB

2 Images
ELISA - Anti-NT-proBNP antibody [15F11] (AB13115)
  • ELISA

Unknown

ELISA - Anti-NT-proBNP antibody [15F11] (AB13115)

NT-proBNP calibration curves using pairs ab14703 [10H4] and ab13111 [5B6] (grey), and ab13115 [15F11] and ab13111 [5B6] (black), (capture : 2 μg/well, detection : Eu-labeled 5B6; 200 ng/well). Antigen was recombinant NT-proBNP. Incubated 1 hr at room temperature

Western blot - Anti-NT-proBNP antibody [15F11] (AB13115)
  • WB

Unknown

Western blot - Anti-NT-proBNP antibody [15F11] (AB13115)

WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11].

Lane 1:

Western blot - Anti-NT-proBNP antibody [15F11] (ab13115)

Lane 1:

Western blot - Anti-proBNP antibody [5B6] (<a href='/en-us/products/primary-antibodies/probnp-antibody-5b6-ab13111'>ab13111</a>)

Predicted band size: 15 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

15F11

Isotype

IgG2b

Carrier free

No

Applications

WB, ELISA

applications

Immunogen

Synthetic Peptide within Human NPPB aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P16860

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

Immunoglobulin concentrations vary from batch to batch. Please contact technical services for the latest information.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Chromatography on protein A Sepharose
Storage buffer
Preservative: 0.09% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NT-proBNP known as N-terminal prohormone of brain natriuretic peptide is a non-active prohormone that measures approximately 8.5 kDa in mass. This prohormone emerges from the cleavage of pro-BNP in cardiomyocytes specifically in the heart ventricles. NT-proBNP levels rise in response to ventricular volume expansion and pressure overload therefore serving as an important indicator of cardiac function. Researchers regularly utilize assays like NT-proBNP ELISA and NT-proBNP monoclonal antibodies for detecting its presence in various biological samples.
Biological function summary

NT-proBNP stands important for cardiovascular homeostasis. It does not participate as part of a complex but plays a role on its own. When released into circulation NT-proBNP functions as an indicator of heart performance indirectly reflecting the activity of the active hormone BNP. Blood vessels dilate under BNP influence reducing systemic vascular resistance and central venous pressure which aids in heart function improvement.

Pathways

NT-proBNP interacts significantly within the cardiac stress response pathways. It plays a focus within the natriuretic peptide system interacting with proteins such as BNP and ANP that help regulate fluid balance and blood pressure. These components together influence pathways that modulate hemodynamic stress and sodium excretion ensuring cardiovascular stability under stress conditions.

Clinicians use NT-proBNP as a biomarker for heart failure and ischemic heart disease. Elevated levels of NT-proBNP correlate with cardiac stress indicating unmet cardiac workload or myocardial ischemia. In these contexts NT-proBNP serves alongside BNP as an indicator of increased heart strain thereby offering insights into disease progression and patient response to therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Brain natriuretic peptide 32. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed : 1672777, PubMed : 17372040, PubMed : 1914098, PubMed : 9458824). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed : 1672777, PubMed : 17349887, PubMed : 17372040, PubMed : 21098034, PubMed : 25339504, PubMed : 9458824). Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion (PubMed : 1914098, PubMed : 9458824). Binds the clearance receptor NPR3 (PubMed : 16870210).. NT-proBNP. May affect cardio-renal homeostasis (PubMed : 17372040). Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32 (PubMed : 17372040).. BNP(3-32). May have a role in cardio-renal homeostasis (PubMed : 17372040). Able to promote the production of cGMP (PubMed : 17372040).
See full target information NPPB
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 25:1987-2014 PubMed38454158

2024

α-Melanocyte-stimulating hormone alleviates pathological cardiac remodeling via melanocortin 5 receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Anni Suominen,Guillem Saldo Rubio,Saku Ruohonen,Zoltán Szabó,Lotta Pohjolainen,Bishwa Ghimire,Suvi T Ruohonen,Karla Saukkonen,Jani Ijas,Sini Skarp,Leena Kaikkonen,Minying Cai,Sharon L Wardlaw,Heikki Ruskoaho,Virpi Talman,Eriika Savontaus,Risto Kerkelä,Petteri Rinne

Journal of molecular and cellular cardiology plus 2:100020 PubMed39802492

2022

Transcriptomics reveal stretched human pluripotent stem cell-derived cardiomyocytes as an advantageous hypertrophy model.

Applications

Unspecified application

Species

Unspecified reactive species

Lotta Pohjolainen,Heikki Ruskoaho,Virpi Talman

STAR protocols 2:100912 PubMed34755117

2021

A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Walter E Knight,Yingqiong Cao,Phoebe Dillon,Kunhua Song

Frontiers in pharmacology 11:553852 PubMed33584253

2021

Pharmacological Protein Kinase C Modulators Reveal a Pro-hypertrophic Role for Novel Protein Kinase C Isoforms in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Lotta Pohjolainen,Julia Easton,Reesha Solanki,Heikki Ruskoaho,Virpi Talman

Archives of toxicology 94:2113-2130 PubMed32185414

2020

GATA4-targeted compound exhibits cardioprotective actions against doxorubicin-induced toxicity in vitro and in vivo: establishment of a chronic cardiotoxicity model using human iPSC-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

S Tuuli Karhu,Sini M Kinnunen,Marja Tölli,Mika J Välimäki,Zoltán Szabó,Virpi Talman,Heikki Ruskoaho

Methods and protocols 2: PubMed31717790

2019

High-Throughput Phenotyping Toolkit for Characterizing Cellular Models of Hypertrophic Cardiomyopathy In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Diogo Mosqueira,Katarzyna Lis-Slimak,Chris Denning

EBioMedicine 24:147-158 PubMed28942281

2017

Antihypertrophic Effects of Small Molecules that Maintain Mitochondrial ATP Levels Under Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroaki Nagai,Tomoko Satomi,Akiko Abiru,Kazumasa Miyamoto,Koji Nagasawa,Minoru Maruyama,Satoshi Yamamoto,Kuniko Kikuchi,Hiromitsu Fuse,Masakuni Noda,Yoshiyuki Tsujihata

Clinical chemistry 57:864-73 PubMed21482747

2011

Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes.

Applications

IP

Species

Human

Jason M Tonne,Jarryd M Campbell,Alessandro Cataliotti,Seiga Ohmine,Tayaramma Thatava,Toshie Sakuma,Fima Macheret,Brenda K Huntley,John C Burnett,Yasuhiro Ikeda
View all publications
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Product promise

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