Mouse Monoclonal BNP antibody. Suitable for ELISA, WB and reacts with Recombinant fragment - Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human NPPB aa 1-50.
Images
![ELISA - Anti-NT-proBNP antibody [15F11] (AB13115), expandable thumbnail](https://content.abcam.com/products/images/nt-probnp-antibody-15f11-ab13115--elisa-img117151.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-NT-proBNP antibody [15F11] (AB13115)")
![Western blot - Anti-NT-proBNP antibody [15F11] (AB13115), expandable thumbnail](https://content.abcam.com/products/images/nt-probnp-antibody-15f11-ab13115--western-blot-img145956.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NT-proBNP antibody [15F11] (AB13115)")
Publications
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- STAR protocols 2:1009122021A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWalter E Knight,Yingqiong Cao,Phoebe Dillon,Kunhua SongPubMed 34755117
- Frontiers in pharmacology 11:5538522021Pharmacological Protein Kinase C Modulators Reveal a Pro-hypertrophic Role for Novel Protein Kinase C Isoforms in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLotta Pohjolainen,Julia Easton,Reesha Solanki,Heikki Ruskoaho,Virpi TalmanPubMed 33584253
- Archives of toxicology 94:2113-21302020GATA4-targeted compound exhibits cardioprotective actions against doxorubicin-induced toxicity in vitro and in vivo: establishment of a chronic cardiotoxicity model using human iPSC-derived cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Tuuli Karhu et. al.PubMed 32185414
- Methods and protocols 2:2019High-Throughput Phenotyping Toolkit for Characterizing Cellular Models of Hypertrophic Cardiomyopathy In Vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiogo Mosqueira et. al.PubMed 31717790
- EBioMedicine 24:147-1582017Antihypertrophic Effects of Small Molecules that Maintain Mitochondrial ATP Levels Under Hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroaki Nagai et. al.PubMed 28942281
- Clinical chemistry 57:864-732011Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human NPPB aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P16860
Reactivity data
Select an application| ELISA | WB | |
|---|---|---|
| Recombinant fragment - Human | Tested | Tested |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Brain natriuretic peptide 32. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed:1672777, PubMed:17372040, PubMed:1914098, PubMed:9458824). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed:1672777, PubMed:17349887, PubMed:17372040, PubMed:21098034, PubMed:25339504, PubMed:9458824). Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion (PubMed:1914098, PubMed:9458824). Binds the clearance receptor NPR3 (PubMed:16870210). NT-proBNP. May affect cardio-renal homeostasis (PubMed:17372040). Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32 (PubMed:17372040). BNP(3-32). May have a role in cardio-renal homeostasis (PubMed:17372040). Able to promote the production of cGMP (PubMed:17372040).
Alternative names
Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, NPPB
Recommended products
Mouse Monoclonal BNP antibody. Suitable for ELISA, WB and reacts with Recombinant fragment - Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human NPPB aa 1-50.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human NPPB aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P16860
- Clone number
- 15F11
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Chromatography on protein A Sepharose
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Immunoglobulin concentrations vary from batch to batch. Please contact technical services for the latest information.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NT-proBNP known as N-terminal prohormone of brain natriuretic peptide is a non-active prohormone that measures approximately 8.5 kDa in mass. This prohormone emerges from the cleavage of pro-BNP in cardiomyocytes specifically in the heart ventricles. NT-proBNP levels rise in response to ventricular volume expansion and pressure overload therefore serving as an important indicator of cardiac function. Researchers regularly utilize assays like NT-proBNP ELISA and NT-proBNP monoclonal antibodies for detecting its presence in various biological samples.
- Biological function summary
NT-proBNP stands important for cardiovascular homeostasis. It does not participate as part of a complex but plays a role on its own. When released into circulation NT-proBNP functions as an indicator of heart performance indirectly reflecting the activity of the active hormone BNP. Blood vessels dilate under BNP influence reducing systemic vascular resistance and central venous pressure which aids in heart function improvement.
- Pathways
NT-proBNP interacts significantly within the cardiac stress response pathways. It plays a focus within the natriuretic peptide system interacting with proteins such as BNP and ANP that help regulate fluid balance and blood pressure. These components together influence pathways that modulate hemodynamic stress and sodium excretion ensuring cardiovascular stability under stress conditions.
- Associated diseases and disorders
Clinicians use NT-proBNP as a biomarker for heart failure and ischemic heart disease. Elevated levels of NT-proBNP correlate with cardiac stress indicating unmet cardiac workload or myocardial ischemia. In these contexts NT-proBNP serves alongside BNP as an indicator of increased heart strain thereby offering insights into disease progression and patient response to therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![ELISA - Anti-NT-proBNP antibody [15F11] (ab13115), expandable thumbnail](https://content.abcam.com/products/images/nt-probnp-antibody-15f11-ab13115--elisa-img117151.jpg "ELISA - Anti-NT-proBNP antibody [15F11] (ab13115)")
ELISA - Anti-NT-proBNP antibody [15F11] (ab13115)
NT-proBNP calibration curves using pairs ab14703 [10H4] and Anti-proBNP antibody [5B6] ab13111 [5B6] (grey), and ab13115 [15F11] and Anti-proBNP antibody [5B6] ab13111 [5B6] (black), (capture: 2 μg/well, detection: Eu-labeled 5B6; 200 ng/well). Antigen was recombinant NT-proBNP. Incubated 1 hr at room temperature
![Western blot - Anti-NT-proBNP antibody [15F11] (ab13115), expandable thumbnail](https://content.abcam.com/products/images/nt-probnp-antibody-15f11-ab13115--western-blot-img145956.jpg "Western blot - Anti-NT-proBNP antibody [15F11] (ab13115)")
Western blot - Anti-NT-proBNP antibody [15F11] (ab13115)
WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - Anti-proBNP antibody [5B6] ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 HRP Anti-proBNP antibody [24E11] ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - Anti-proBNP antibody [5B6] ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 HRP Anti-proBNP antibody [24E11] ab13123 [24E11].
Lane 1: Western blot - Anti-NT-proBNP antibody [15F11] (ab13115)
Lane 1: Western blot - Anti-proBNP antibody [5B6] (Anti-proBNP antibody [5B6] ab13111)
Predicted band size: 15 kDa
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