Rabbit Recombinant Monoclonal BNP antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
sELISA | |
---|---|
Mouse | Tested |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes - |
Brain natriuretic peptide 45Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed:8182124). May also function as a paracrine antifibrotic factor in the heart (PubMed:10737768). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed:8182124). Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis (By similarity).
Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, Nppb
Rabbit Recombinant Monoclonal BNP antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR25816-71
Affinity purification Protein A
This antibody has 15% cross reaction with mouse full length proBNP.
Blue Ice
+4°C
+4°C
ab322929 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse NT-proBNP. The recommended pair for sandwich ELISA is:
Capture: ab322929, Mouse NT-proBNP Capture Antibody (unconjugated)
Detector: Anti-NT-proBNP antibody [EPR25816-7] - BSA and Azide free (Detector) ab322930, Mouse NT-proBNP Detector Antibody (unconjugated)
The reference range value is 3.13-200 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Interpolated concentration of native NT-proBNP was measured at different sample concentrations. Undiluted samples are as follows: mouse plasma 100% and mouse heart supernatant (cultured for 2 days) 50%. Sample dilutions are made in antibody diluent CP2 (Antibody Diluent CP2 ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
Sandwich ELISA of Mouse NT-proBNP Antibody Pair - BSA and Azide free ab322928 with the capture antibody ab322929 dilution at 2 ug/mL and detector antibody Anti-NT-proBNP antibody [EPR25816-7] - BSA and Azide free (Detector) ab322930 dilution at 0.5 ug/mL.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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