JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB190214

Anti-NT5C1A antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal NT5C1A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Cytosolic 5'-nucleotidase 1A aa 150-200.

View Alternative Names

Cytosolic 5'-nucleotidase 1A, cN1A, 5'-deoxynucleotidase, Cytosolic 5'-nucleotidase IA, cN-I, cN-IA, NT5C1A

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-NT5C1A antibody (AB190214)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NT5C1A antibody (AB190214)

Immunofluorescent analysis of A549 cells labeling NT5C1A using ab190214 at 1/100 dilution. The image on the right is treated with the synthesized peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NT5C1A antibody (AB190214)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NT5C1A antibody (AB190214)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human skeletal muscle tissue labeling NT5C1A using ab190214 at 10 μg/ml.

Western blot - Anti-NT5C1A antibody (AB190214)
  • WB

Supplier Data

Western blot - Anti-NT5C1A antibody (AB190214)

All lanes:

Western blot - Anti-NT5C1A antibody (ab190214) at 1/500 dilution

Lane 1:

MCF7 cell lysate

Lane 2:

HepG2 cell lysate

Lane 3:

MCF7 cell lysate treated with the synthesized peptide

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Cytosolic 5'-nucleotidase 1A aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q9BXI3

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NT5C1A also known as cytosolic 5'-nucleotidase 1A is an enzyme involved in the catabolism of nucleotides. It dephosphorylates nucleoside monophosphates into nucleosides and inorganic phosphate. The molecular mass of NT5C1A is approximately 41 kDa. It is expressed in various tissues with notable presence in muscle tissue and peripheral blood mononuclear cells.
Biological function summary

NT5C1A contributes to the regulation of nucleotide pools in cells impacting DNA and RNA synthesis and degradation processes. It functions independently and does not seem to be a part of a larger complex. Its activity ensures balanced nucleotide levels which is essential for proper cellular function and replication.

Pathways

NT5C1A participates in the purine metabolism pathway by influencing the concentration of nucleosides and nucleotides. It works in coordination with other enzymes such as adenosine deaminase and xanthine oxidase within these metabolic processes. NT5C1A's role is pivotal in maintaining nucleotide homeostasis during cellular proliferation and apoptosis.

NT5C1A has been linked to sporadic inclusion body myositis a muscle disorder characterized by muscle inflammation and weakening. Some studies suggest a connection with autoimmune diseases; however research is still evolving in this area. In these conditions NT5C1A interacts with proteins involved in immune response modulation hinting at its role in disease development.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the hydrolysis of ribonucleotide and deoxyribonucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside (PubMed : 11133996, PubMed : 34814800, PubMed : 7599155, PubMed : 8967393). AMP is the major substrate but can also hydrolyze dCMP and IMP (PubMed : 11133996, PubMed : 34814800, PubMed : 7599155, PubMed : 8967393).
See full target information Cytosolic 5'-nucleotidase 1A
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Npj viruses 2:6 PubMed40295745

2024

The Wnt/β-catenin pathway is important for replication of SARS-CoV-2 and other pathogenic RNA viruses.

Applications

Unspecified application

Species

Unspecified reactive species

Zaikun Xu,Mohamed Elaish,Cheung Pang Wong,Bardes B Hassan,Joaquin Lopez-Orozco,Alberto Felix-Lopez,Natacha S Ogando,Les Nagata,Lara K Mahal,Anil Kumar,Joyce A Wilson,Ryan Noyce,Irv Mayers,Christopher Power,David Evans,Tom C Hobman

Metabolites 13: PubMed36676954

2022

Urinary ATP Levels Are Controlled by Nucleotidases Released from the Urothelium in a Regulated Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro Gutierrez Cruz,Mafalda S L Aresta Branco,Brian A Perrino,Kenton M Sanders,Violeta N Mutafova-Yambolieva

Frontiers in physiology 13:918100 PubMed35784885

2022

Mechanosensitive Hydrolysis of ATP and ADP in Lamina Propria of the Murine Bladder by Membrane-Bound and Soluble Nucleotidases.

Applications

Unspecified application

Species

Unspecified reactive species

Mafalda S L Aresta Branco,Alejandro Gutierrez Cruz,Jacob Dayton,Brian A Perrino,Violeta N Mutafova-Yambolieva
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
  • 19
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Assay Kits

AB65393

LDH Assay Kit (Cytotoxicity)

Biochemical assay - LDH Assay Kit (Cytotoxicity) (AB65393)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com