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AB276067

Anti-NT5C3 antibody [009] - BSA and Azide free

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Rabbit Recombinant Monoclonal PN-I antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NT5C3A aa 1-300.

View Alternative Names

NT5C3, P5N1, UMPH1, HSPC233, NT5C3A, Cytosolic 5'-nucleotidase 3A, 7-methylguanosine phosphate-specific 5'-nucleotidase, Cytosolic 5'-nucleotidase 3, Cytosolic 5'-nucleotidase III, Pyrimidine 5'-nucleotidase 1, Uridine 5'-monophosphate hydrolase 1, p36, 7-methylguanosine nucleotidase, cN-III, P5'N-1, P5N-1, PN-I

2 Images
Immunoprecipitation - Anti-NT5C3 antibody [009] - BSA and Azide free (AB276067)
  • IP

Unknown

Immunoprecipitation - Anti-NT5C3 antibody [009] - BSA and Azide free (AB276067)

NT5C3 was immunoprecipitated from 0.5 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) and Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with 0.5 μL ab276067 and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using ab276067 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.

Developed using the odssey technique.

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Immunoprecipitation - Anti-NT5C3 antibody [009] - BSA and Azide free (ab276067)

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Western blot - Anti-NT5C3 antibody [009] - BSA and Azide free (AB276067)
  • WB

Unknown

Western blot - Anti-NT5C3 antibody [009] - BSA and Azide free (AB276067)

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Western blot - Anti-NT5C3 antibody [009] - BSA and Azide free (ab276067) at 1/500 dilution

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HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Secondary

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Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

009

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human NT5C3A aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9H0P0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>1-2 μL/mg of lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nucleotidase which shows specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP) (PubMed : 24603684). CMP seems to be the preferred substrate (PubMed : 15968458).
See full target information NT5C3A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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