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AB228683

Anti-NTE antibody - C-terminal

3

(1 Review)

|

(2 Publications)

Rabbit Polyclonal NTE antibody. C-terminal. Suitable for WB, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PNPLA6.

View Alternative Names

NTE, PNPLA6, Patatin-like phospholipase domain-containing protein 6, Neuropathy target esterase

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (AB228683)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (AB228683)

Paraffin-embedded rat forebrain tissue stained for NTE with ab228683 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (AB228683)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (AB228683)

Paraffin-embedded mouse hindbrain tissue stained for NTE with ab228683 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-NTE antibody - C-terminal (AB228683)
  • WB

Supplier Data

Western blot - Anti-NTE antibody - C-terminal (AB228683)

5% SDS-PAGE

All lanes:

Western blot - Anti-NTE antibody - C-terminal (ab228683) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extracts at 30 µg

Lane 2:

SK-N-SH (human neuroblastoma cell line) whole cell extracts at 30 µg

Lane 3:

IMR32 (human neuroblast cell line) whole cell extracts at 30 µg

Lane 4:

SK-N-AS (human neuroblastoma cell line) whole cell extracts at 30 µg

Predicted band size: 150 kDa

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Western blot - Anti-NTE antibody - C-terminal (AB228683)
  • WB

Supplier Data

Western blot - Anti-NTE antibody - C-terminal (AB228683)

5% SDS-PAGE

All lanes:

Western blot - Anti-NTE antibody - C-terminal (ab228683) at 1/1000 dilution

All lanes:

Rat brain tissue extract at 50 µg

Predicted band size: 150 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PNPLA6. The exact immunogen used to generate this antibody is proprietary information.

Q8IY17

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Neuropathy Target Esterase (NTE) also known as PNPLA6 is an important enzyme that catalyzes the hydrolysis of specific phospholipids. This enzyme is approximately 150 kDa in mass. It is widely expressed in neuronal tissues but is found in various other tissues such as liver and kidneys as well. By removing specific side groups from phospholipids NTE regulates lipid metabolism and membrane dynamics which is essential for maintaining cellular function and integrity.
Biological function summary

The Neuropathy Target Esterase functions in the detoxification of neuropathic organophosphates and plays critical roles in neural development and maintenance. NTE is part of a complex involved in the regulation of membrane phospholipid homeostasis. This complex acts to monitor and maintain the composition of membranes which is important for cell signaling and adhesion processes. The absence or malfunction of NTE can lead to disruptions in these processes indicating its importance in normal neuronal activity.

Pathways

NTE participates in phospholipid biosynthesis and degradation pathways. It interacts with proteins involved in membrane trafficking and signaling such as phospholipase D. These pathways are critical for lipid signaling and membrane dynamics linking NTE to key processes in cellular communication and response. NTE's association with these pathways emphasizes its role in maintaining lipid balance and cellular communication through its enzymatic activity.

NTE mutations or dysfunctions connect to complex conditions such as hereditary spastic paraplegia and motor neuron disease. These mutations can disrupt its known interactions with proteins like REEP1 and spastin within the nerve cells. Such conditions often involve the degeneration of motor neurons leading to progressive motor dysfunction. Understanding NTE's role in these diseases may provide insights for therapeutic interventions targeting these neurodegenerative disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Catalyzes the hydrolysis of several naturally occurring membrane-associated lipids (PubMed : 11927584). Hydrolyzes lysophospholipids and monoacylglycerols, preferring the 1-acyl to the 2-acyl isomer. Does not catalyze hydrolysis of di- or triacylglycerols or fatty acid amides (PubMed : 11927584).
See full target information PNPLA6
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2221 PubMed40082403

2025

PNPLA6 regulates retinal homeostasis by choline through phospholipid turnover.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Ono,Yoshitaka Taketomi,Takayoshi Higashi,Hiroyasu Sato,Chika Mochizuki-Ono,Yuki Nagasaki,Takashi Ueta,Takashi Miyai,Suzumi M Tokuoka,Yoshiya Oda,Yasumasa Nishito,Tomio Ono,Choji Taya,Satoru Arata,Sumiko Watanabe,Tomoyoshi Soga,Tetsuya Hirabayashi,Makoto Aihara,Makoto Murakami

BMC immunology 23:16 PubMed35379170

2022

ThPOK inhibits the immune escape of gastric cancer cells by inducing STPG1 to inactivate the ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Chen,Lili Jiang,Lingli Xia,Gang Zhang,Lan Chen
View all publications
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