Rabbit Polyclonal NTE antibody. C-terminal. Suitable for WB, IHC-P and reacts with Rat, Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human PNPLA6.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | IHC-P | |
---|---|---|
Human | Tested | Expected |
Mouse | Expected | Tested |
Rat | Tested | Tested |
Chicken | Predicted | Predicted |
Cow | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Cow | Dilution info - | Notes - |
Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Catalyzes the hydrolysis of several naturally occurring membrane-associated lipids (PubMed:11927584). Hydrolyzes lysophospholipids and monoacylglycerols, preferring the 1-acyl to the 2-acyl isomer. Does not catalyze hydrolysis of di- or triacylglycerols or fatty acid amides (PubMed:11927584).
NTE, PNPLA6, Patatin-like phospholipase domain-containing protein 6, Neuropathy target esterase
Rabbit Polyclonal NTE antibody. C-terminal. Suitable for WB, IHC-P and reacts with Rat, Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human PNPLA6.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The protein Neuropathy Target Esterase (NTE) also known as PNPLA6 is an important enzyme that catalyzes the hydrolysis of specific phospholipids. This enzyme is approximately 150 kDa in mass. It is widely expressed in neuronal tissues but is found in various other tissues such as liver and kidneys as well. By removing specific side groups from phospholipids NTE regulates lipid metabolism and membrane dynamics which is essential for maintaining cellular function and integrity.
The Neuropathy Target Esterase functions in the detoxification of neuropathic organophosphates and plays critical roles in neural development and maintenance. NTE is part of a complex involved in the regulation of membrane phospholipid homeostasis. This complex acts to monitor and maintain the composition of membranes which is important for cell signaling and adhesion processes. The absence or malfunction of NTE can lead to disruptions in these processes indicating its importance in normal neuronal activity.
NTE participates in phospholipid biosynthesis and degradation pathways. It interacts with proteins involved in membrane trafficking and signaling such as phospholipase D. These pathways are critical for lipid signaling and membrane dynamics linking NTE to key processes in cellular communication and response. NTE's association with these pathways emphasizes its role in maintaining lipid balance and cellular communication through its enzymatic activity.
NTE mutations or dysfunctions connect to complex conditions such as hereditary spastic paraplegia and motor neuron disease. These mutations can disrupt its known interactions with proteins like REEP1 and spastin within the nerve cells. Such conditions often involve the degeneration of motor neurons leading to progressive motor dysfunction. Understanding NTE's role in these diseases may provide insights for therapeutic interventions targeting these neurodegenerative disorders.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Terms & Conditions.
Paraffin-embedded mouse hindbrain tissue stained for NTE with ab228683 at 1/500 dilution in immunohistochemical analysis.
5% SDS-PAGE
All lanes: Western blot - Anti-NTE antibody - C-terminal (ab228683) at 1/1000 dilution
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extracts at 30 µg
Lane 2: SK-N-SH (human neuroblastoma cell line) whole cell extracts at 30 µg
Lane 3: IMR32 (human neuroblast cell line) whole cell extracts at 30 µg
Lane 4: SK-N-AS (human neuroblastoma cell line) whole cell extracts at 30 µg
Developed using the ECL technique.
Predicted band size: 150 kDa
Paraffin-embedded rat forebrain tissue stained for NTE with ab228683 at 1/500 dilution in immunohistochemical analysis.
5% SDS-PAGE
All lanes: Western blot - Anti-NTE antibody - C-terminal (ab228683) at 1/1000 dilution
All lanes: Rat brain tissue extract at 50 µg
Developed using the ECL technique.
Predicted band size: 150 kDa
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