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Rabbit Polyclonal NTE antibody. C-terminal. Suitable for WB, IHC-P and reacts with Rat, Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human PNPLA6.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (AB228683), expandable thumbnail
  • Western blot - Anti-NTE antibody - C-terminal (AB228683), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (AB228683), expandable thumbnail
  • Western blot - Anti-NTE antibody - C-terminal (AB228683), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human PNPLA6. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IY17

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Expected
Mouse
Expected
Tested
Rat
Tested
Tested
Chicken
Predicted
Predicted
Cow
Predicted
Predicted

Tested
Tested

Species

Rat

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chicken, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Species

Rat

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chicken, Cow

Dilution info

-

Notes

-

Target data

Function

Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Catalyzes the hydrolysis of several naturally occurring membrane-associated lipids (PubMed:11927584). Hydrolyzes lysophospholipids and monoacylglycerols, preferring the 1-acyl to the 2-acyl isomer. Does not catalyze hydrolysis of di- or triacylglycerols or fatty acid amides (PubMed:11927584).

Alternative names

Recommended products

Rabbit Polyclonal NTE antibody. C-terminal. Suitable for WB, IHC-P and reacts with Rat, Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human PNPLA6.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human PNPLA6. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IY17
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein Neuropathy Target Esterase (NTE) also known as PNPLA6 is an important enzyme that catalyzes the hydrolysis of specific phospholipids. This enzyme is approximately 150 kDa in mass. It is widely expressed in neuronal tissues but is found in various other tissues such as liver and kidneys as well. By removing specific side groups from phospholipids NTE regulates lipid metabolism and membrane dynamics which is essential for maintaining cellular function and integrity.

Biological function summary

The Neuropathy Target Esterase functions in the detoxification of neuropathic organophosphates and plays critical roles in neural development and maintenance. NTE is part of a complex involved in the regulation of membrane phospholipid homeostasis. This complex acts to monitor and maintain the composition of membranes which is important for cell signaling and adhesion processes. The absence or malfunction of NTE can lead to disruptions in these processes indicating its importance in normal neuronal activity.

Pathways

NTE participates in phospholipid biosynthesis and degradation pathways. It interacts with proteins involved in membrane trafficking and signaling such as phospholipase D. These pathways are critical for lipid signaling and membrane dynamics linking NTE to key processes in cellular communication and response. NTE's association with these pathways emphasizes its role in maintaining lipid balance and cellular communication through its enzymatic activity.

Associated diseases and disorders

NTE mutations or dysfunctions connect to complex conditions such as hereditary spastic paraplegia and motor neuron disease. These mutations can disrupt its known interactions with proteins like REEP1 and spastin within the nerve cells. Such conditions often involve the degeneration of motor neurons leading to progressive motor dysfunction. Understanding NTE's role in these diseases may provide insights for therapeutic interventions targeting these neurodegenerative disorders.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (ab228683), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (ab228683)

    Paraffin-embedded mouse hindbrain tissue stained for NTE with ab228683 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-NTE antibody - C-terminal (ab228683), expandable thumbnail

    Western blot - Anti-NTE antibody - C-terminal (ab228683)

    5% SDS-PAGE

    All lanes: Western blot - Anti-NTE antibody - C-terminal (ab228683) at 1/1000 dilution

    Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extracts at 30 µg

    Lane 2: SK-N-SH (human neuroblastoma cell line) whole cell extracts at 30 µg

    Lane 3: IMR32 (human neuroblast cell line) whole cell extracts at 30 µg

    Lane 4: SK-N-AS (human neuroblastoma cell line) whole cell extracts at 30 µg

    Developed using the ECL technique.

    Predicted band size: 150 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (ab228683), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (ab228683)

    Paraffin-embedded rat forebrain tissue stained for NTE with ab228683 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-NTE antibody - C-terminal (ab228683), expandable thumbnail

    Western blot - Anti-NTE antibody - C-terminal (ab228683)

    5% SDS-PAGE

    All lanes: Western blot - Anti-NTE antibody - C-terminal (ab228683) at 1/1000 dilution

    All lanes: Rat brain tissue extract at 50 µg

    Developed using the ECL technique.

    Predicted band size: 150 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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