AB254146

Anti-NTF2 antibody [5A3]

Recombinant
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(3 Publications)

Mouse Recombinant Monoclonal NTF2 antibody. Suitable for ICC/IF, WB and reacts with Human, Rat, Mouse samples. Cited in 3 publications.

View Alternative Names

NTF2, NUTF2, Nuclear transport factor 2, NTF-2, Placental protein 15, PP15

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NTF2 antibody [5A3] (AB254146)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-NTF2 antibody [5A3] (AB254146)

Immunocytochemical analysis of 4% paraformaldehyde-fixed, 100% methanol-permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling NTF2 with ab254146 at 1/50 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in HeLa cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1 : ab254146 at a 1/50 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2 : ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

Lab

Western blot - Anti-NTF2 antibody [5A3] (AB254146)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NTF2 antibody [5A3] (ab254146) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Lane 3:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 4:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

Lane 5:

IMR-32 (human neuroblastoma neuroblast), whole cell lysate at 10 µg

Lane 6:

F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 7:

L6 (rat skeletal muscle myoblast), whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 14 kDa

false

Exposure time: 8s

Carrier free

Anti-NTF2 antibody [5A3] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5A3

Isotype

IgG2

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NTF2 protein also known as Nuclear Transport Factor 2 has an important role in the transport mechanism between the cytoplasm and the nucleus. It has a molecular weight of approximately 14 kDa. NTF2 exists in multiple tissues including in the liver and brain. This protein facilitates the transport of molecules specifically enzymes and regulatory proteins through the nuclear pore complex.

Biological function summary

The NTF2 protein acts as a transport receptor that primarily mediates the import of RanGDP into the nucleus. It forms complexes with other proteins to ensure efficient transport functionality. The interaction with Ran a small GTPase is critical in maintaining the gradient necessary for nucleocytoplasmic transport. NTF2 operates by binding to nuclear pore complexes and guiding proteins into the nucleus.

Pathways

NTF2 holds importance in nucleocytoplasmic transport and Ran-mediated transport pathways. It interacts closely with other molecules such as Ran and importin β facilitating efficient nuclear import cycles. This protein also plays an associated role in cellular processes like RNA export and signal transduction by influencing how proteins translocate across the nuclear membrane.

NTF2 connects to certain neurological disorders including Alzheimer's disease. Abnormalities in nucleocytoplasmic transport can influence disease pathology and NTF2's malfunction may lead to the accumulation of misfolded proteins impacting neural function. Additionally NTF2 involvement can be observed in cancer where changes in transport capacity may lead to altered cell cycle regulation. In cancer pathology it relates to proteins like p53 which when dysregulated promotes tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. Interacts with GDP-bound RAN in the cytosol, recruits it to the nuclear pore complex via its interaction with nucleoporins and promotes its nuclear import.

See full target information NUTF2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Neurochemical research 50:106 PubMed40011296

2025

Pyrrolidine Dithiocarbamate Ameliorates Sepsis-Associated Encephalopathy by Inhibiting Autophagy and Inflammation in the Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Lu,Zhiyi Zuo

OncoTargets and therapy 14:5455-5467 PubMed35221694

2021

Identification of NUTF2 as a Candidate Diagnostic and Prognostic Biomarker Associated with Immune Infiltration in Head and Neck Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhang,Ying Gao

Molecular biology of the cell 11:703-19 PubMed10679025

2000

Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran.

Applications

Unspecified application

Species

Unspecified reactive species

S M Steggerda,B E Black,B M Paschal

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NTF2 antibody [5A3]

Immunocytochemistry/ Immunofluorescence - Anti-NTF2 antibody [5A3] (AB254146)

Western blot - Anti-NTF2 antibody [5A3] (AB254146)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise