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Mouse Recombinant Monoclonal NTF2 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

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Images

Western blot - Anti-NTF2 antibody [5A3] - BSA and Azide free (AB255903), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NTF2 antibody [5A3] - BSA and Azide free (AB255903), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Tested
Tested
Not recommended
Mouse
Not recommended
Tested
Not recommended
Rat
Expected
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Mediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. Interacts with GDP-bound RAN in the cytosol, recruits it to the nuclear pore complex via its interaction with nucleoporins and promotes its nuclear import.

Alternative names

NTF2, NUTF2, Nuclear transport factor 2, NTF-2, Placental protein 15, PP15

Recommended products

Mouse Recombinant Monoclonal NTF2 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
5A3
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab255903 is the carrier-free version of Anti-NTF2 antibody [5A3] ab254146.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The NTF2 protein also known as Nuclear Transport Factor 2 has an important role in the transport mechanism between the cytoplasm and the nucleus. It has a molecular weight of approximately 14 kDa. NTF2 exists in multiple tissues including in the liver and brain. This protein facilitates the transport of molecules specifically enzymes and regulatory proteins through the nuclear pore complex.

Biological function summary

The NTF2 protein acts as a transport receptor that primarily mediates the import of RanGDP into the nucleus. It forms complexes with other proteins to ensure efficient transport functionality. The interaction with Ran a small GTPase is critical in maintaining the gradient necessary for nucleocytoplasmic transport. NTF2 operates by binding to nuclear pore complexes and guiding proteins into the nucleus.

Pathways

NTF2 holds importance in nucleocytoplasmic transport and Ran-mediated transport pathways. It interacts closely with other molecules such as Ran and importin β facilitating efficient nuclear import cycles. This protein also plays an associated role in cellular processes like RNA export and signal transduction by influencing how proteins translocate across the nuclear membrane.

Associated diseases and disorders

NTF2 connects to certain neurological disorders including Alzheimer's disease. Abnormalities in nucleocytoplasmic transport can influence disease pathology and NTF2's malfunction may lead to the accumulation of misfolded proteins impacting neural function. Additionally NTF2 involvement can be observed in cancer where changes in transport capacity may lead to altered cell cycle regulation. In cancer pathology it relates to proteins like p53 which when dysregulated promotes tumorigenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com