Anti-NTH1 antibody [22H10]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-NTH1 antibody [22H10] (AB314192)

Overlay Peak curve showing HepG2 (Human liver hepatocellular carcinoma cell line) cells stained with ab314192 (pink line) at 1 : 100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1x10E6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1 : 200 dilution for 35min at 4°C. Control antibody (green line) was rabbit IgG (1ug/1x10E6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [22H10] (AB314192)

Immunohistochemical analysis of ab314192 diluted at 1 : 100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NTH1 antibody [22H10] (AB314192)

Immunofluorescence staining of PC-3 (Human prostate adenocarcinoma cell line) cells with ab314192 at 1 : 50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor® 604-conjugated Goat Anti-Rabbit IgG(H+L).

• WB

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Western blot - Anti-NTH1 antibody [22H10] (AB314192)

All lanes:

Western blot - Anti-NTH1 antibody [22H10] (ab314192) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysate

Lane 2:

HEK293 whole cell lysate

Lane 3:

PC-3 whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Observed band size: 35 kDa

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