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AB70726

Anti-NTH1 antibody [2660C1a]

5

(4 Reviews)

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(3 Publications)

Mouse Monoclonal NTH1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NTHL1.

View Alternative Names

NTH1, OCTS3, NTHL1, Endonuclease III-like protein 1, hNTH1, Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase, DNA glycosylase/AP lyase

2 Images
Western blot - Anti-NTH1 antibody [2660C1a] (AB70726)
  • WB

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Western blot - Anti-NTH1 antibody [2660C1a] (AB70726)

All lanes:

Western blot - Anti-NTH1 antibody [2660C1a] (ab70726) at 0.2 µg/mL

Lane 1:

Whole cell lysate from human HEK293 cells at 25 µg

Lane 2:

Whole cell lysate from mouse NIH3T3 cells at 25 µg

Lane 3:

Whole cell lysate from rat F2408 cells at 25 µg

Predicted band size: 34 kDa

Observed band size: 37 kDa

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Western blot - Anti-NTH1 antibody [2660C1a] (AB70726)
  • WB

Unknown

Western blot - Anti-NTH1 antibody [2660C1a] (AB70726)

All lanes:

Western blot - Anti-NTH1 antibody [2660C1a] (ab70726) at 0.2 µg/mL

All lanes:

immunised recombinant human NTH1 protein

Predicted band size: 34 kDa

Observed band size: 35 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2660C1a

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human NTHL1. The exact immunogen used to generate this antibody is proprietary information.

P78549

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab70726 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NTH1 protein also known as endonuclease III-like protein 1 functions as a DNA glycosylase involved in base excision repair. It plays a role in the repair of oxidative DNA damage. NTH1 has a molecular mass of approximately 33 kDa. This protein is located in various tissues throughout the human body but it shows higher expression in the testes heart and skeletal muscle. These expression patterns suggest its significance in oxidative stress response across different body systems.
Biological function summary

NTH1 removes oxidized pyrimidines from damaged DNA as part of its role in cellular repair mechanisms. As a component of the base excision repair pathway it tackles oxidative lesions to prevent mutation accumulation. It does not act as a complex with other proteins but operates after being recruited to relevant DNA sites requiring repair. This essential function keeps genomic integrity intact and allows for regular cellular processes.

Pathways

NTH1 plays an important role within the base excision repair and DNA damage response pathways. It cooperates with proteins such as APE1 which prepares the site for subsequent repair steps ensuring proper DNA maintenance. NTH1’s involvement in base excision repair specifically addresses small-scale oxidative damage highlighting its role in safeguarding genetic information. These pathways are fundamental for normal cell function and response to environmental stressors.

NTH1 has links to conditions involving accumulated DNA damage such as cancer and neurodegenerative diseases. DNA repair deficiency due to NTH1 malfunction can contribute to the onset of such diseases. The protein interacts with partners like OGG1 which also plays a role in oxidative DNA lesion recognition and repair to manage DNA stability. Understanding the dysfunction of NTH1 in these contexts helps in exploring potential therapeutic targets and diagnostic markers for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage (PubMed : 29610152, PubMed : 9927729). The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Has also 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption.
See full target information NTHL1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36232914

2022

TRIM26 Maintains Cell Survival in Response to Oxidative Stress through Regulating DNA Glycosylase Stability.

Applications

Unspecified application

Species

Unspecified reactive species

Sifaddin M R Konis,Jonathan R Hughes,Jason L Parsons

Molecular and cellular biology 38: PubMed29610152

2018

NTH1 Is a New Target for Ubiquitylation-Dependent Regulation by TRIM26 Required for the Cellular Response to Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah C Williams,Jason L Parsons

Nucleic acids research 46:4515-4532 PubMed29522130

2018

Overexpression of the base excision repair NTHL1 glycosylase causes genomic instability and early cellular hallmarks of cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin L Limpose,Kelly S Trego,Zhentian Li,Sara W Leung,Altaf H Sarker,Jason A Shah,Suresh S Ramalingam,Erica M Werner,William S Dynan,Priscilla K Cooper,Anita H Corbett,Paul W Doetsch
View all publications
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