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AB191413

Anti-NTH1 antibody [EPR15930]

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(6 Publications)

Rabbit Recombinant Monoclonal NTH1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 6 publications.

View Alternative Names

NTH1, OCTS3, NTHL1, Endonuclease III-like protein 1, hNTH1, Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase, DNA glycosylase/AP lyase

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (AB191413)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (AB191413)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Negative control utilised PBS instead of ab191413.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (AB191413)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (AB191413)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Negative control utilised PBS instead of ab191413.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-NTH1 antibody [EPR15930] (AB191413)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NTH1 antibody [EPR15930] (AB191413)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling NTH1 with ab191413 at 1/150 dilution, followed by Goat anti rabbit IgG (Alexa Fluor488) secondary antibody at 1/200 dilution (ab150077). Nuclear counter stain is DAPI (blue).

The two negative controls are ab191413 at 1/250 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (AB191413)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (AB191413)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Negative control utilised PBS instead of ab191413.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-NTH1 antibody [EPR15930] (AB191413)
  • WB

Supplier Data

Western blot - Anti-NTH1 antibody [EPR15930] (AB191413)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-NTH1 antibody [EPR15930] (ab191413) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

  • Carrier free

    Anti-NTH1 antibody [EPR15930] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15930

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/150", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NTH1 protein also known as endonuclease III-like protein 1 functions as a DNA glycosylase involved in base excision repair. It plays a role in the repair of oxidative DNA damage. NTH1 has a molecular mass of approximately 33 kDa. This protein is located in various tissues throughout the human body but it shows higher expression in the testes heart and skeletal muscle. These expression patterns suggest its significance in oxidative stress response across different body systems.
Biological function summary

NTH1 removes oxidized pyrimidines from damaged DNA as part of its role in cellular repair mechanisms. As a component of the base excision repair pathway it tackles oxidative lesions to prevent mutation accumulation. It does not act as a complex with other proteins but operates after being recruited to relevant DNA sites requiring repair. This essential function keeps genomic integrity intact and allows for regular cellular processes.

Pathways

NTH1 plays an important role within the base excision repair and DNA damage response pathways. It cooperates with proteins such as APE1 which prepares the site for subsequent repair steps ensuring proper DNA maintenance. NTH1’s involvement in base excision repair specifically addresses small-scale oxidative damage highlighting its role in safeguarding genetic information. These pathways are fundamental for normal cell function and response to environmental stressors.

NTH1 has links to conditions involving accumulated DNA damage such as cancer and neurodegenerative diseases. DNA repair deficiency due to NTH1 malfunction can contribute to the onset of such diseases. The protein interacts with partners like OGG1 which also plays a role in oxidative DNA lesion recognition and repair to manage DNA stability. Understanding the dysfunction of NTH1 in these contexts helps in exploring potential therapeutic targets and diagnostic markers for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage (PubMed : 29610152, PubMed : 9927729). The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Also has 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption.
See full target information NTHL1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

DNA repair 133:103610 PubMed38101146

2023

Targeted nuclear irradiation with a proton microbeam induces oxidative DNA base damage and triggers the recruitment of DNA glycosylases OGG1 and NTH1.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Robeska,Kévin Lalanne,François Vianna,Haser Hasan Sutcu,Andriy Khobta,Didier Busso,J Pablo Radicella,Anna Campalans,Céline Baldeyron

Nucleic acids research 51:4982-4994 PubMed37026475

2023

Requirement of transcription-coupled nucleotide excision repair for the removal of a specific type of oxidatively induced DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Leen Sarmini,Mohammed Meabed,Eirini Emmanouil,George Atsaves,Elena Robeska,Bolesław T Karwowski,Anna Campalans,Thanasis Gimisis,Andriy Khobta

Frontiers in cardiovascular medicine 9:1053697 PubMed36620624

2022

5mC modification patterns provide novel direction for early acute myocardial infarction detection and personalized therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqun Guo,Hua Jiang,Jinlong Wang,Ping Li,Xiaoquan Zeng,Tao Zhang,Jianyi Feng,Ruqiong Nie,Yulong Liu,Xiaobian Dong,Qingsong Hu

The Journal of physiology 597:869-887 PubMed30556208

2018

Induced in vivo knockdown of the Brca1 gene in skeletal muscle results in skeletal muscle weakness.

Applications

Unspecified application

Species

Unspecified reactive species

Michael D Tarpey,Ana P Valencia,Kathryn C Jackson,Adam J Amorese,Nicholas P Balestrieri,Randall H Renegar,Stephen J P Pratt,Terence E Ryan,Joseph M McClung,Richard M Lovering,Espen E Spangenburg

Nucleic acids research 46:5664-5677 PubMed29750271

2018

APE1 deficiency promotes cellular senescence and premature aging features.

Applications

Unspecified application

Species

Unspecified reactive species

Mengxia Li,Xiao Yang,Xianfeng Lu,Nan Dai,Shiheng Zhang,Yi Cheng,Lei Zhang,Yuxin Yang,Yie Liu,Zhenzhou Yang,Dong Wang,David M Wilson

DNA repair 57:91-97 PubMed28709015

2017

Human cells contain a factor that facilitates the DNA glycosylase-mediated excision of oxidized bases from occluded sites in nucleosomes.

Applications

Unspecified application

Species

Unspecified reactive species

R L Maher,C G Marsden,A M Averill,S S Wallace,J B Sweasy,D S Pederson
View all publications
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Product promise

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