Rabbit Recombinant Monoclonal S6A17 antibody. Carrier free. Suitable for WB, IP, IHC-P, IHC-Fr, ICC/IF and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (AB313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-frozen-sections-img418701.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (AB313748)")
![Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (AB313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-frozen-sections-img418700.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (AB313748)")
![Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (AB313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-frozen-sections-img418699.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (AB313748)")
![Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (AB313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-frozen-sections-img418698.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (AB313748)")
![Immunocytochemistry/ Immunofluorescence - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (AB313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunocytochemistry-immunofluorescence-img418695.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (AB313748)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | IHC-P | IHC-Fr | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected | Expected | Not recommended |
| Mouse | Tested | Tested | Tested | Tested | Tested | Not recommended |
| Rat | Tested | Expected | Tested | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Synaptic vesicle transporter with apparent selectivity for neutral amino acids. The transport is sodium-coupled but chloride-independent, likely driven by the proton electrochemical gradient generated by vacuolar H(+)-ATPase in an overall electrogenic mechanism. May contribute to the synaptic uptake of neurotransmitter precursors in a process coupled in part to vesicle exocytosis.
Alternative names
NTT4, SLC6A17, Sodium-dependent neutral amino acid transporter SLC6A17, Sodium-dependent neurotransmitter transporter NTT4, Solute carrier family 6 member 17
Recommended products
Rabbit Recombinant Monoclonal S6A17 antibody. Carrier free. Suitable for WB, IP, IHC-P, IHC-Fr, ICC/IF and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28520-13
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NTT4 also known as SLC6A17 is a sodium-dependent neurotransmitter transporter. This protein has a mass of approximately 70 kDa and is found primarily in the brain's cortical and hippocampal regions. It facilitates the uptake of neutral amino acids by neurons. The transport mechanism involves moving neurotransmitter molecules across cell membranes influencing synaptic communication.
- Biological function summary
The function of NTT4 involves its role in the reuptake of amino acids critical for neurotransmission. As a member of the solute carrier family 6 (SLC6) NTT4 operates as part of a larger transporter complex system. It ensures that neurons maintain proper concentrations of neurotransmitters which are necessary for effective synaptic transmission and neuronal communication.
- Pathways
NTT4 functions within the neurotransmitter transport and synaptic signaling pathways. It closely interacts with proteins such as glutamate and GABA transporters contributing to maintaining excitatory and inhibitory balance in the central nervous system. This balance is important for normal cognitive functions and prevents neurological dysregulation.
- Associated diseases and disorders
NTT4 is linked to neurological conditions like schizophrenia and epilepsy. A disruption in this transporter’s function can lead to imbalances in neurotransmitter levels which are implicated in these diseases. Related proteins like dopamine and serotonin transporters often show functional linkages with NTT4 in the pathological states of these psychiatric and neurological disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
17 product images
![Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-frozen-sections-img418701.jpg "Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh) tissue labeling NTT4 with Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/50 (10.9 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on rat kidney (PMID: 23672601). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-NTT4 antibody [EPR28520-13] ab313747 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.![Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-frozen-sections-img418700.jpg "Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling NTT4 with Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/50 (10.9 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-NTT4 antibody [EPR28520-13] ab313747 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.![Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-frozen-sections-img418699.jpg "Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney (fresh) tissue labeling NTT4 with Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/50 (10.9 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse kidney (PMID: 23672601). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-NTT4 antibody [EPR28520-13] ab313747 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.![Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-frozen-sections-img418698.jpg "Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Immunohistochemistry (Frozen sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling NTT4 with Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/50 (10.9 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-NTT4 antibody [EPR28520-13] ab313747 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.![Immunocytochemistry/ Immunofluorescence - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunocytochemistry-immunofluorescence-img418695.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Immunocytochemistry/ Immunofluorescence - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling NTT4 with Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/50 (10.9 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418692.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling NTT4 with Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/500 (1.09 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on human liver. The section was incubated with Anti-NTT4 antibody [EPR28520-13] ab313747 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418691.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling NTT4 with Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/500 (1.09 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on human kidney. The section was incubated with Anti-NTT4 antibody [EPR28520-13] ab313747 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418690.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling NTT4 with Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/1000 (0.545 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat cerebellum. The section was incubated with Anti-NTT4 antibody [EPR28520-13] ab313747 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418689.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling NTT4 with Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/1000 (0.545 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat cerebrum. The section was incubated with Anti-NTT4 antibody [EPR28520-13] ab313747 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418688.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling NTT4 with Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/1000 (0.545 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebellum. The section was incubated with Anti-NTT4 antibody [EPR28520-13] ab313747 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418687.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling NTT4 with Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/1000 (0.545 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebrum. The section was incubated with Anti-NTT4 antibody [EPR28520-13] ab313747 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418686.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling NTT4 with Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/500 (1.09 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human cerebrum. The section was incubated with Anti-NTT4 antibody [EPR28520-13] ab313747 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.![Immunoprecipitation - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunoprecipitation-img418673.jpg "Immunoprecipitation - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Immunoprecipitation - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
NTT4 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 ug
Lane 2: Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NTT4 antibody [EPR28520-13] ab313747 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 secondsAll lanes: Immunoprecipitation - Anti-NTT4 antibody [EPR28520-13] (Anti-NTT4 antibody [EPR28520-13] ab313747) at 1/30 dilution
All lanes: Mouse brain tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 8s
![Immunoprecipitation - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunoprecipitation-img418672.jpg "Immunoprecipitation - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Immunoprecipitation - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
NTT4 was immunoprecipitated from 0.35 mg Human cerebellum tissue lysate with Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-NTT4 antibody [EPR28520-13] ab313747 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human cerebellum tissue lysate 10 ug
Lane 2: Human cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NTT4 antibody [EPR28520-13] ab313747 in human cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 secondsAll lanes: Immunoprecipitation - Anti-NTT4 antibody [EPR28520-13] (Anti-NTT4 antibody [EPR28520-13] ab313747) at 1/30 dilution
All lanes: Human cerebellum tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 8s
![Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--western-blot-img418659.jpg "Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
NTT4 detected a shift from 75kDa to 65kDa band after deglycosylation treatment.
In Western blot, anti- H3 antibody (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) loading control staining at 1/100000 dilution.
Exposure time: 15 secondsAll lanes: Western blot - Anti-NTT4 antibody [EPR28520-13] (Anti-NTT4 antibody [EPR28520-13] ab313747) at 1/1000 dilution
Lane 1: Untreated mouse brain tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate treated with Protein treated with Peptide:N-glycosidase F (PNGase F) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 75 kDa
Exposure time: 15s
![Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--western-blot-img418657.jpg "Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: liver, kidney (PMID: 23672601).
The expression profile observed is consistent with what has been described in literature (PMID:23672601).
The identity of the lower MW band at approximately 25 kDa is unknown.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Samples are non-boiled as boiling may cause protein aggregation.
Exposure time: Lane1-2: 15 seconds; Lane 3-5: 26 secondsAll lanes: Western blot - Anti-NTT4 antibody [EPR28520-13] (Anti-NTT4 antibody [EPR28520-13] ab313747) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 20 µg
Lane 2: Human hypothalamus tissue lysate at 20 µg
Lane 3: Human spinal cord tissue lysate at 20 µg
Lane 4: Human kidney tissue lysate at 20 µg
Lane 5: Human liver tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 75 kDa
![Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--western-blot-img418658.jpg "Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)
This data was developed using Anti-NTT4 antibody [EPR28520-13] ab313747, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: liver, kidney (PMID: 23672601).
The expression profile observed is consistent with what has been described in literature (PMID:23672601).
The identity of the lower MW band at approximately 25 kDa is unknown.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Samples are non-boiled as boiling may cause protein aggregation.
Exposure time: 26 secondsAll lanes: Western blot - Anti-NTT4 antibody [EPR28520-13] (Anti-NTT4 antibody [EPR28520-13] ab313747) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse cerebellum tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Mouse kidney tissue lysate at 20 µg
Lane 5: Rat brain tissue lysate at 20 µg
Lane 6: Rat kidney tissue lysate at 20 µg
Lane 7: Mouse hippocampus tissue lysate at 20 µg
Lane 8: Mouse spinal cord tissue lysate at 20 µg
Lane 9: Rat cerebellum tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 75 kDa
Exposure time: 26s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418692.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418691.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418690.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418689.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418688.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418687.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418686.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
![Immunoprecipitation - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunoprecipitation-img418673.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
![Immunoprecipitation - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--immunoprecipitation-img418672.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
![Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--western-blot-img418659.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
![Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--western-blot-img418657.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
![Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748), expandable thumbnail](https://content.abcam.com/products/images/ntt4-antibody-epr28520-13-bsa-and-azide-free-ab313748--western-blot-img418658.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NTT4 antibody [EPR28520-13] - BSA and Azide free (ab313748)")
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