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Rabbit Recombinant Monoclonal ARK5 antibody. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human samples.

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Images

Western blot - Anti-NUAK1/ARK5 antibody [EPR28626-53] (AB319148), expandable thumbnail
  • Western blot - Anti-NUAK1/ARK5 antibody [EPR28626-53] (AB319148), expandable thumbnail
  • Dot Blot - Anti-NUAK1/ARK5 antibody [EPR28626-53] (AB319148), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotWBICC/IFIHC-P
Human
Expected
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate in transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed:25329316).

Alternative names

ARK5, KIAA0537, OMPHK1, NUAK1, NUAK family SNF1-like kinase 1, AMPK-related protein kinase 5, Omphalocele kinase 1

Recommended products

Rabbit Recombinant Monoclonal ARK5 antibody. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28626-53
Purification technique
Affinity purification Protein A
Specificity

This antibody does not cross-react with human NUAK2 by dot blot.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NUAK1 also known as ARK5 is a protein kinase with an approximate molecular mass of 72 kDa. It belongs to the AMP-activated protein kinase (AMPK) family. This kinase is expressed in various tissues including the brain liver and skeletal muscle. NUAK1 plays a mechanical role by phosphorylating substrates which leads to modulation of their activity and localization. It interacts with cellular structures by influencing cytoskeletal dynamics cell adhesion and polarity.

Biological function summary

NUAK1 influences cell survival proliferation and metabolism by interacting with other proteins. It is not part of a stable complex but its activity affects many cellular processes. NUAK1 has a part in cellular responses to metabolic stress which helps cells to maintain energy homeostasis. By regulating processes such as apoptosis and autophagy NUAK1 impacts critical cellular functions.

Pathways

NUAK1 participates in the LKB1-AMPK signaling pathway. It acts downstream of LKB1 a tumor suppressor that activates AMPK-related kinases. This pathway is central to energy balance and metabolic control linking to cellular metabolism through substrates like ACC and mTOR. NUAK1 also has interactions with protein kinases like PKA through related signaling which further integrates its functions across cellular processes.

Associated diseases and disorders

NUAK1 associates with cancer and metabolic disorders. In cancer its overactivity may drive tumor growth and metastasis by promoting cell survival pathways. NUAK1 has links with proteins such as MYC and Cyclin D which are important for cell cycle regulation. In metabolic disorders abnormalities in NUAK1 signaling can affect glucose uptake and insulin sensitivity associating it with conditions like type 2 diabetes. These findings make NUAK1 an interesting target for therapeutic research in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-NUAK1/ARK5 antibody [EPR28626-53] (ab319148), expandable thumbnail

    Western blot - Anti-NUAK1/ARK5 antibody [EPR28626-53] (ab319148)

    Low expression: HUVEC (PMID: 32873786), HepG2 (PMID: 14982852), Raji.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-NUAK1/ARK5 antibody [EPR28626-53] (ab319148) at 1/1000 dilution

    Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate at 60 µg

    Lane 2: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 60 µg

    Lane 3: NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 60 µg

    Lane 4: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 60 µg

    Lane 5: L-929 (mouse connective tissue fibroblast) whole cell lysate at 60 µg

    Lane 6: U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 60 µg

    Lane 7: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 60 µg

    Lane 8: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 60 µg

    Lane 9: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 60 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 74 kDa, 36 kDa

    Exposure time: 81s

  • Western blot - Anti-NUAK1/ARK5 antibody [EPR28626-53] (ab319148), expandable thumbnail

    Western blot - Anti-NUAK1/ARK5 antibody [EPR28626-53] (ab319148)

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-NUAK1/ARK5 antibody [EPR28626-53] (ab319148) at 1/1000 dilution

    All lanes: Human brain tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 74 kDa

    Exposure time: 37s

  • Dot Blot - Anti-NUAK1/ARK5 antibody [EPR28626-53] (ab319148), expandable thumbnail

    Dot Blot - Anti-NUAK1/ARK5 antibody [EPR28626-53] (ab319148)

    Dot blot analysis of NUAK1/ARK5 using ab319148 at 1:1000 (0.503 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human NUAK2.

    All lanes: Dot Blot - Anti-NUAK1/ARK5 antibody [EPR28626-53] (ab319148) at 1/1000 dilution

    Lane 1: His-tagged human NUAK1/ARK5 fragment

    Lane 2: Untagged human NUAK2 fragment

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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