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AB126048

Anti-NUAK2 antibody

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(2 Publications)

Rabbit Polyclonal NUAK2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUAK2 aa 1-250.

View Alternative Names

OMPHK2, SNARK, NUAK2, NUAK family SNF1-like kinase 2, Omphalocele kinase 2, SNF1/AMP kinase-related kinase

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUAK2 antibody (AB126048)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUAK2 antibody (AB126048)

ab126048 at 1/500 dilution, staining NUAK2 in Paraffin-embedded Human Gastric carcinoma tissue by Immnunohistochemistry

Western blot - Anti-NUAK2 antibody (AB126048)
  • WB

Unknown

Western blot - Anti-NUAK2 antibody (AB126048)

7.5% SDS PAGE

All lanes:

Western blot - Anti-NUAK2 antibody (ab126048) at 1/1000 dilution

All lanes:

NT2D1 whole cell lysate at 30 µg

Predicted band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NUAK2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9H093

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUAK2 also known as SNARK or NUAK family kinase 2 is a serine/threonine-protein kinase involved in various cellular processes. With a molecular mass of approximately 74 kDa NUAK2 is expressed in multiple tissues notably in the brain skeletal muscle and heart. This kinase plays an important role in regulating cellular energy balance and responds to metabolic stresses acting as a critical mediator in the cellular response to nutrient availability.
Biological function summary

NUAK2 plays an active role in modulating cellular adhesion migration and proliferation. It is not part of a well-defined complex but interacts with several cellular components to exert its functions. NUAK2 participates in the regulation of the actin cytoskeleton impacting cell structure and movement and is involved in maintaining cellular homeostasis through metabolic regulation. It also influences signaling pathways that regulate cellular growth and survival.

Pathways

NUAK2 plays significant roles in the AMP-activated protein kinase (AMPK) signaling pathway and the insulin signaling pathway. As a downstream effector of these pathways NUAK2 impacts important metabolic processes by regulating energy utilization and promoting cell survival under stress conditions. It relates to proteins such as LKB1 which is an upstream kinase that phosphorylates and activates NUAK2 and AMPK which shares signaling pathways impacting metabolism and cell growth.

Various studies have linked NUAK2 to cancer and metabolic disorders. Overexpression of NUAK2 has been observed in several types of cancer including breast and colorectal cancer where it may contribute to tumorigenic processes by promoting cell proliferation and migration. Additionally NUAK2 is connected to hepatic steatosis influencing lipid accumulation in liver cells. In cancer NUAK2 interacts with proteins like MYPT1 which assists in regulating substrate phosphorylation further contributing to its role in cancer biology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Phosphorylates LATS1 and LATS2. Plays a key role in neural tube closure during embryonic development through LATS2 phosphorylation and regulation of the nuclear localization of YAP1 a critical downstream regulatory target in the Hippo signaling pathway (PubMed : 32845958).
See full target information NUAK2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109406 PubMed38510132

2024

The NF-κB/NUAK2 signaling axis regulates pancreatic cancer progression by targeting SMAD2/3.

Applications

Unspecified application

Species

Unspecified reactive species

Ruobing Wang,Dan Su,Yueze Liu,Hua Huang,Jiangdong Qiu,Zhe Cao,Gang Yang,Hao Chen,Wenhao Luo,Jinxin Tao,Guihu Weng,Taiping Zhang

Cells 11: PubMed35011609

2022

KANPHOS: A Database of Kinase-Associated Neural Protein Phosphorylation in the Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Rijwan Uddin Ahammad,Tomoki Nishioka,Junichiro Yoshimoto,Takayuki Kannon,Mutsuki Amano,Yasuhiro Funahashi,Daisuke Tsuboi,Md Omar Faruk,Yukie Yamahashi,Kiyofumi Yamada,Taku Nagai,Kozo Kaibuchi
View all publications
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Product promise

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