Rabbit Recombinant Monoclonal NUAK2 antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.
Images
![Western blot - Anti-NUAK2 antibody [EPR28627-717] (AB322265), expandable thumbnail](https://content.abcam.com/products/images/nuak2-antibody-epr28627-717-ab322265--western-blot-img438175.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NUAK2 antibody [EPR28627-717] (AB322265)")
![Western blot - Anti-NUAK2 antibody [EPR28627-717] (AB322265), expandable thumbnail](https://content.abcam.com/products/images/nuak2-antibody-epr28627-717-ab322265--western-blot-img438174.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NUAK2 antibody [EPR28627-717] (AB322265)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Transfected cell lysate - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Transfected cell lysate - Human | Dilution info - | Notes - |
Target data
Function
Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Phosphorylates LATS1 and LATS2. Plays a key role in neural tube closure during embryonic development through LATS2 phosphorylation and regulation of the nuclear localization of YAP1 a critical downstream regulatory target in the Hippo signaling pathway (PubMed:32845958).
Alternative names
OMPHK2, SNARK, NUAK2, NUAK family SNF1-like kinase 2, Omphalocele kinase 2, SNF1/AMP kinase-related kinase
Rabbit Recombinant Monoclonal NUAK2 antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR28627-717
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NUAK2 also known as SNARK or NUAK family kinase 2 is a serine/threonine-protein kinase involved in various cellular processes. With a molecular mass of approximately 74 kDa NUAK2 is expressed in multiple tissues notably in the brain skeletal muscle and heart. This kinase plays an important role in regulating cellular energy balance and responds to metabolic stresses acting as a critical mediator in the cellular response to nutrient availability.
- Biological function summary
NUAK2 plays an active role in modulating cellular adhesion migration and proliferation. It is not part of a well-defined complex but interacts with several cellular components to exert its functions. NUAK2 participates in the regulation of the actin cytoskeleton impacting cell structure and movement and is involved in maintaining cellular homeostasis through metabolic regulation. It also influences signaling pathways that regulate cellular growth and survival.
- Pathways
NUAK2 plays significant roles in the AMP-activated protein kinase (AMPK) signaling pathway and the insulin signaling pathway. As a downstream effector of these pathways NUAK2 impacts important metabolic processes by regulating energy utilization and promoting cell survival under stress conditions. It relates to proteins such as LKB1 which is an upstream kinase that phosphorylates and activates NUAK2 and AMPK which shares signaling pathways impacting metabolism and cell growth.
- Associated diseases and disorders
Various studies have linked NUAK2 to cancer and metabolic disorders. Overexpression of NUAK2 has been observed in several types of cancer including breast and colorectal cancer where it may contribute to tumorigenic processes by promoting cell proliferation and migration. Additionally NUAK2 is connected to hepatic steatosis influencing lipid accumulation in liver cells. In cancer NUAK2 interacts with proteins like MYPT1 which assists in regulating substrate phosphorylation further contributing to its role in cancer biology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-NUAK2 antibody [EPR28627-717] (ab322265), expandable thumbnail](https://content.abcam.com/products/images/nuak2-antibody-epr28627-717-ab322265--western-blot-img438175.jpg "Western blot - Anti-NUAK2 antibody [EPR28627-717] (ab322265)")
Western blot - Anti-NUAK2 antibody [EPR28627-717] (ab322265)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human NUAK1.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-NUAK2 antibody [EPR28627-717] (ab322265) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human NUAK1 expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human NUAK2 expression vector containing a GFP-tag, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 125 kDa, 15 kDa, 100 kDa
Exposure time: 3s
![Western blot - Anti-NUAK2 antibody [EPR28627-717] (ab322265), expandable thumbnail](https://content.abcam.com/products/images/nuak2-antibody-epr28627-717-ab322265--western-blot-img438174.jpg "Western blot - Anti-NUAK2 antibody [EPR28627-717] (ab322265)")
Western blot - Anti-NUAK2 antibody [EPR28627-717] (ab322265)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Jurkat, U-937
This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-NUAK2 antibody [EPR28627-717] (ab322265) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole lysate at 50 µg
Lane 2: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole lysate at 50 µg
Lane 3: TT (human thyroid carcinoma epithelial cell) whole lysate at 50 µg
Lane 4: U-937 (human histiocytic lymphoma monocyte) whole lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 75 kDa, 36 kDa
Exposure time: 180s
Downloads
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