Anti-NUAK2 antibody [EPR28627-717] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-NUAK2 antibody [EPR28627-717] - BSA and Azide free (AB324800)

This data was developed using ab322265, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Jurkat U-937

This blot was developed using a high-sensitivity ECL substrate allowing for the detection of proteins in the mid-femtogram range.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-NUAK2 antibody [EPR28627-717] (<a href='/en-us/products/primary-antibodies/nuak2-antibody-epr28627-717-ab322265'>ab322265</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole lysate at 50 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole lysate at 50 µg

Lane 3:

TT (human thyroid carcinoma epithelial cell) whole lysate at 50 µg

Lane 4:

U-937 (human histiocytic lymphoma monocyte) whole lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa,36 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-NUAK2 antibody [EPR28627-717] - BSA and Azide free (AB324800)

This data was developed using ab322265, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human NUAK1.

In Western blot Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

In Western blot Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-NUAK2 antibody [EPR28627-717] (<a href='/en-us/products/primary-antibodies/nuak2-antibody-epr28627-717-ab322265'>ab322265</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human NUAK1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human NUAK2 expression vector containing a GFP-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 125 kDa,15 kDa,100 kDa

false

Exposure time: 3s