AB171077

Anti-NUB1 antibody [EPR10716]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal NUB1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NYREN18, NUB1, NEDD8 ultimate buster 1, Negative regulator of ubiquitin-like proteins 1, Renal carcinoma antigen NY-REN-18

3 Images

Flow Cytometry (Intracellular) - Anti-NUB1 antibody [EPR10716] (AB171077)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NUB1 antibody [EPR10716] (AB171077)

Intracellular flow cytometric analysis of permeabilized Raji cells labeling NUB1 using ab171077 at 1/10 dilution (red) or a rabbit IgG negative (green).

Immunocytochemistry/ Immunofluorescence - Anti-NUB1 antibody [EPR10716] (AB171077)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUB1 antibody [EPR10716] (AB171077)

Immunofluorescent staining of HeLa cells labeling NUB1 using ab171077 at 1/100 dilution.

Supplier Data

Western blot - Anti-NUB1 antibody [EPR10716] (AB171077)

All lanes:

Western blot - Anti-NUB1 antibody [EPR10716] (ab171077) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 71 kDa

false

Carrier free

Anti-NUB1 antibody [EPR10716] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10716

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUB1 also known as NEDD8 ultimate buster-1 functions as a negative regulator of NEDD8 conjugation. This protein weighs around 65 kDa. Researchers find NUB1 to be widely expressed in the human body with higher expression levels observed in organs like the heart and brain. NUB1 interacts mainly with ubiquitin-like proteins including NEDD8 and facilitates their degradation by the proteasome reducing NEDD8's presence in cells.

Biological function summary

NUB1 influences protein homeostasis and signal transduction. It associates with the proteasome to target and eliminate neddylated proteins contributing to the regulation of protein turnover. NUB1 also forms part of a complex that affects the cellular levels of specific proteins through the ubiquitin-proteasome system. This action helps maintain cellular protein balance and impacts various cellular functions.

Pathways

The ubiquitin-proteasome system revolves around NUB1 as it regulates neddylation and ubiquitination processes. NUB1 connects to the neddylation pathway which includes proteins like NEDD8 and UBA3 influencing protein activities and degradation. Through its association with ubiquitination it regulates the stability and function of key cellular proteins ensuring proper cell function and preventing harmful protein accumulation.

NUB1 shows a connection with neurodegenerative diseases and certain cancers. In neurodegenerative conditions like Alzheimer's disease NUB1's regulatory function impacts protein aggregates' formation potentially influencing disease progression. NUB1 also interacts with proteins linked to cancer pathways such as ubiquitin implicating it in the development of certain malignancies. Adequate function and regulation of NUB1 may therefore play a role in mitigating these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.

See full target information NUB1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 132: PubMed36066968

2022

BAF60c prevents abdominal aortic aneurysm formation through epigenetic control of vascular smooth muscle cell homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Guizhen Zhao,Yang Zhao,Haocheng Lu,Ziyi Chang,Hongyu Liu,Huilun Wang,Wenying Liang,Yuhao Liu,Tianqing Zhu,Oren Rom,Yanhong Guo,Lin Chang,Bo Yang,Minerva T Garcia-Barrio,Jiandie D Lin,Y Eugene Chen,Jifeng Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUB1 antibody [EPR10716]

Flow Cytometry (Intracellular) - Anti-NUB1 antibody [EPR10716] (AB171077)

Immunocytochemistry/ Immunofluorescence - Anti-NUB1 antibody [EPR10716] (AB171077)

Western blot - Anti-NUB1 antibody [EPR10716] (AB171077)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise