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AB250040

Anti-NUB1 antibody [EPR10717] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NUB1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NYREN18, NUB1, NEDD8 ultimate buster 1, Negative regulator of ubiquitin-like proteins 1, Renal carcinoma antigen NY-REN-18

2 Images
Flow Cytometry (Intracellular) - Anti-NUB1 antibody [EPR10717] - BSA and Azide free (AB250040)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NUB1 antibody [EPR10717] - BSA and Azide free (AB250040)

This data was developed using ab178414, the same antibody clone in a different buffer formulation.

Intracellular flow cytometrical analysis of permeabilized Jurkat cells labeling NYREN18 with ab178414 at 1/10 (red) or a rabbit IgG (negative) (green).

Western blot - Anti-NUB1 antibody [EPR10717] - BSA and Azide free (AB250040)
  • WB

Supplier Data

Western blot - Anti-NUB1 antibody [EPR10717] - BSA and Azide free (AB250040)

This data was developed using ab178414, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NUB1 antibody [EPR10717] (<a href='/en-us/products/primary-antibodies/nub1-antibody-epr10717-ab178414'>ab178414</a>) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 71 kDa

Observed band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10717

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250040 is the carrier-free version of ab178414.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUB1 also known as NEDD8 ultimate buster-1 functions as a negative regulator of NEDD8 conjugation. This protein weighs around 65 kDa. Researchers find NUB1 to be widely expressed in the human body with higher expression levels observed in organs like the heart and brain. NUB1 interacts mainly with ubiquitin-like proteins including NEDD8 and facilitates their degradation by the proteasome reducing NEDD8's presence in cells.
Biological function summary

NUB1 influences protein homeostasis and signal transduction. It associates with the proteasome to target and eliminate neddylated proteins contributing to the regulation of protein turnover. NUB1 also forms part of a complex that affects the cellular levels of specific proteins through the ubiquitin-proteasome system. This action helps maintain cellular protein balance and impacts various cellular functions.

Pathways

The ubiquitin-proteasome system revolves around NUB1 as it regulates neddylation and ubiquitination processes. NUB1 connects to the neddylation pathway which includes proteins like NEDD8 and UBA3 influencing protein activities and degradation. Through its association with ubiquitination it regulates the stability and function of key cellular proteins ensuring proper cell function and preventing harmful protein accumulation.

NUB1 shows a connection with neurodegenerative diseases and certain cancers. In neurodegenerative conditions like Alzheimer's disease NUB1's regulatory function impacts protein aggregates' formation potentially influencing disease progression. NUB1 also interacts with proteins linked to cancer pathways such as ubiquitin implicating it in the development of certain malignancies. Adequate function and regulation of NUB1 may therefore play a role in mitigating these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.
See full target information NUB1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:65 PubMed39972431

2025

Mechanism of histone demethylase KDM5A in osteoporotic fracture healing through epigenetic regulation of the miR-495/SKP2/Runx2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoran Li,Junyan Zhang,Tingting Xu,Zhiying Hao,Yadong Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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