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AB235924

Anti-NUBPL antibody

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(2 Publications)

Rabbit Polyclonal NUBPL antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NUBPL.

View Alternative Names

C14orf127, NUBPL, Iron-sulfur cluster transfer protein NUBPL, IND1 homolog, Nucleotide-binding protein-like, huInd1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBPL antibody (AB235924)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBPL antibody (AB235924)

Paraffin-embedded human liver cancer tissue stained for NUBPL with ab235924 (1/100 dilution) in immunohistochemical analysis.

Western blot - Anti-NUBPL antibody (AB235924)
  • WB

Supplier Data

Western blot - Anti-NUBPL antibody (AB235924)

All lanes:

Western blot - Anti-NUBPL antibody (ab235924) at 1/500 dilution

All lanes:

A431 (Human epidermoid carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 34 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBPL antibody (AB235924)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBPL antibody (AB235924)

Paraffin-embedded human colon cancer tissue stained for NUBPL with ab235924 (1/100 dilution) in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NUBPL.

Q8TB37

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUBPL also known as nucleotide-binding protein-like and IND1 is a mitochondrial matrix protein with a molecular mass of approximately 34 kDa. It is expressed in various tissues being notably present in high-energy demand tissues like heart and skeletal muscle. Mechanically NUBPL plays a critical role in the assembly of complex I of the mitochondrial respiratory chain by aiding the incorporation of Fe-S clusters which are essential for catalytic activity.
Biological function summary

NUBPL functions as a chaperone-like protein ensuring proper assembly of complex I which is part of the mitochondrial respiratory chain. NUBPL operates as a component of the mitochondrial iron-sulfur (Fe-S) cluster assembly complex. It directly interacts with Fe-S protein synthesis an essential part of cellular respiration. The precise regulation of this complex facilitates energy production within cells by supporting efficient electron transport.

Pathways

NUBPL is integral to the mitochondrial oxidative phosphorylation pathway a process that generates ATP the main energy currency of cells. It associates with the iron-sulfur (Fe-S) cluster biogenesis pathway working alongside proteins such as ISCU and NFS1. These interactions ensure the effective transfer and stabilization of Fe-S clusters necessary for mitochondrial function and metabolic processes.

NUBPL mutations have been linked to mitochondrial complex I deficiency which can lead to a spectrum of neurodegenerative conditions including Leigh syndrome. The disease often involves interactions with proteins such as NDUFS4 another complex I assembly factor. Additionally possible links may exist between NUBPL dysfunction and mitochondrial diseases characterized by impaired energy production and accumulation of metabolic intermediates highlighting the protein's importance in maintaining cellular energy homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Iron-sulfur cluster transfer protein involved in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) (PubMed : 19752196). May deliver one or more Fe-S clusters to complex I subunits (PubMed : 19752196).
See full target information NUBPL
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1456649 PubMed39896807

2025

Disulfidptosis-related gene signatures as prognostic biomarkers and predictors of immunotherapy response in HNSCC.

Applications

Unspecified application

Species

Unspecified reactive species

Haotian Qin,Juan Xu,Yaohang Yue,Meiling Chen,Zheng Zhang,Panpan Xu,Yan Zheng,Hui Zeng,Jian Weng,Jun Yang,Fei Yu

Genes & development 38:738-754 PubMed39168636

2024

The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Jiun C Chen,Govinal Badiger Bhaskara,Yue Lu,Kevin Lin,Sharon Y R Dent
View all publications
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