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AB171741

Anti-NUBPL antibody [EPR11833]

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(3 Publications)

Rabbit Recombinant Monoclonal NUBPL antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

C14orf127, NUBPL, Iron-sulfur cluster transfer protein NUBPL, IND1 homolog, Nucleotide-binding protein-like, huInd1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-NUBPL antibody [EPR11833] (AB171741)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUBPL antibody [EPR11833] (AB171741)

Immunofluorescent analysis of HepG2 cells labeling NUBPL using ab171741 at 1/50 dilution.

Immunoprecipitation - Anti-NUBPL antibody [EPR11833] (AB171741)
  • IP

Supplier Data

Immunoprecipitation - Anti-NUBPL antibody [EPR11833] (AB171741)

Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling NUBPL, or 1X PBS (Lane 2 : negative control) using ab171741 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-NUBPL antibody [EPR11833] (ab171741)

Predicted band size: 34 kDa

false

Western blot - Anti-NUBPL antibody [EPR11833] (AB171741)
  • WB

Supplier Data

Western blot - Anti-NUBPL antibody [EPR11833] (AB171741)

All lanes:

Western blot - Anti-NUBPL antibody [EPR11833] (ab171741) at 1/10000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Predicted band size: 34 kDa

false

  • Carrier free

    Anti-NUBPL antibody [EPR11833] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11833

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUBPL also known as nucleotide-binding protein-like and IND1 is a mitochondrial matrix protein with a molecular mass of approximately 34 kDa. It is expressed in various tissues being notably present in high-energy demand tissues like heart and skeletal muscle. Mechanically NUBPL plays a critical role in the assembly of complex I of the mitochondrial respiratory chain by aiding the incorporation of Fe-S clusters which are essential for catalytic activity.
Biological function summary

NUBPL functions as a chaperone-like protein ensuring proper assembly of complex I which is part of the mitochondrial respiratory chain. NUBPL operates as a component of the mitochondrial iron-sulfur (Fe-S) cluster assembly complex. It directly interacts with Fe-S protein synthesis an essential part of cellular respiration. The precise regulation of this complex facilitates energy production within cells by supporting efficient electron transport.

Pathways

NUBPL is integral to the mitochondrial oxidative phosphorylation pathway a process that generates ATP the main energy currency of cells. It associates with the iron-sulfur (Fe-S) cluster biogenesis pathway working alongside proteins such as ISCU and NFS1. These interactions ensure the effective transfer and stabilization of Fe-S clusters necessary for mitochondrial function and metabolic processes.

NUBPL mutations have been linked to mitochondrial complex I deficiency which can lead to a spectrum of neurodegenerative conditions including Leigh syndrome. The disease often involves interactions with proteins such as NDUFS4 another complex I assembly factor. Additionally possible links may exist between NUBPL dysfunction and mitochondrial diseases characterized by impaired energy production and accumulation of metabolic intermediates highlighting the protein's importance in maintaining cellular energy homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Iron-sulfur cluster transfer protein involved in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) (PubMed : 19752196). May deliver one or more Fe-S clusters to complex I subunits (PubMed : 19752196).
See full target information NUBPL
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102090 PubMed38187140

2024

Altered CELF4 splicing factor enhances pancreatic neuroendocrine tumors aggressiveness influencing mTOR and everolimus response.

Applications

Unspecified application

Species

Unspecified reactive species

Emilia Alors-Pérez,Sergio Pedraza-Arevalo,Ricardo Blázquez-Encinas,Víctor García-Vioque,Antonio Agraz-Doblas,Elena M Yubero-Serrano,Marina E Sánchez-Frías,Raquel Serrano-Blanch,María Ángeles Gálvez-Moreno,Francisco Gracia-Navarro,Manuel D Gahete,Álvaro Arjona-Sánchez,Raúl M Luque,Alejandro Ibáñez-Costa,Justo P Castaño

Aging cell 22:e13954 PubMed37614052

2023

Alterations of lipid-mediated mitophagy result in aging-dependent sensorimotor defects.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Oleinik,Onder Albayram,Mohamed Faisal Kassir,F Cansu Atilgan,Chase Walton,Eda Karakaya,John Kurtz,Alexander Alekseyenko,Habeeb Alsudani,Megan Sheridan,Zdzislaw M Szulc,Besim Ogretmen

Orphanet journal of rare diseases 17:386 PubMed36280881

2022

Early embryonic lethality in complex I associated p.L104P Nubpl mutant mice.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Cheng,James Cleak,Lan Weiss,Heather Cater,Michelle Stewart,Sara Wells,Rod Carlo Columbres,Alyaa Shmara,C Alejandra Morato Torres,Faria Zafar,Birgitt Schüle,Jonathan Neumann,Eli Hatchwell,Virginia Kimonis
View all publications
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