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AB154262

Anti-NUCB1 antibody

5

(1 Review)

|

(3 Publications)

Rabbit Polyclonal NUCB1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUCB1 aa 200 to C-terminus.

View Alternative Names

NUC, NUCB1, Nucleobindin-1, CALNUC

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-NUCB1 antibody (AB154262)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUCB1 antibody (AB154262)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling NUCB1 with ab154262 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB1 antibody (AB154262)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB1 antibody (AB154262)

Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue labeling NUCB1 with ab154262 at 1/500 dilution.

Western blot - Anti-NUCB1 antibody (AB154262)
  • WB

Unknown

Western blot - Anti-NUCB1 antibody (AB154262)

10% SDS PAGE

All lanes:

Western blot - Anti-NUCB1 antibody (ab154262) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 54 kDa

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Western blot - Anti-NUCB1 antibody (AB154262)
  • WB

Unknown

Western blot - Anti-NUCB1 antibody (AB154262)

10% SDS PAGE

All lanes:

Western blot - Anti-NUCB1 antibody (ab154262) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NUCB1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q02818

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein NUCB1 also known as Nucleobindin-1 functions mechanically as a calcium-binding protein. It has approximately a mass of 55 kDa and belongs to a group of calcium-binding proteins containing EF-hand motifs. NUCB1 expresses in a variety of tissues notably the nervous system and endocrine tissues. The protein comprises several functional domains including a signal peptide helix-loop-helix motif and nuclear localization signal.
Biological function summary

NUCB1 participates in the regulation of cellular calcium homeostasis and signaling pathways. It is not a part of a multi-protein complex but it acts as a molecular chaperone influencing the stability and function of other proteins within the cell. NUCB1 also plays a role in secretory pathways within the cell which is relevant to its expression in endocrine tissues. Its regulatory functions in calcium signaling are critical for cellular activities such as secretion and cell division.

Pathways

NUCB1 interacts with cellular signaling mechanisms particularly those related to intracellular calcium dynamics. It is involved in pathways like the calcium signaling pathway and the insulin signaling pathway. Within these pathways NUCB1 associates with proteins such as calmodulin which also binds calcium and contributes to signal transduction. The protein modulates activities within these pathways affecting how cells respond to external signals.

NUCB1 exhibits associations with certain conditions linked to disturbed calcium regulation. One such disorder is Alzheimer's disease where alterations in calcium signaling play a role in disease progression. The protein furthermore relates to type 2 diabetes involving its role in the insulin signaling pathway. In these contexts NUCB1 connects to other proteins such as amyloid precursor protein in Alzheimer's disease highlighting disruptions in calcium homeostasis mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major calcium-binding protein of the Golgi which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates alpha subunits of guanine nucleotide-binding proteins (G proteins) (By similarity).
See full target information NUCB1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 51:102185 PubMed39522303

2024

Prognostic significance of combined PCPE-1 and α-fetoprotein for hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ruhua Zhang,Wanqi Chen,Xuelan Peng,Zhiguang Zhang,Shangjiu Yang,Li Zhong

Computational and structural biotechnology journal 21:1510-1522 PubMed36851918

2023

Identification of and as ovarian endometriosis risk-associated genes using integrative genomic analyses and functional experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhou Huang,Jie Luo,Yue Zhang,Tao Zhang,Xiangwei Fei,Liqing Chen,Yingfan Zhu,Songyue Li,Caiyun Zhou,Kaihong Xu,Yunlong Ma,Jun Lin,Jianhong Zhou

Frontiers in cell and developmental biology 9:641836 PubMed33855021

2021

NUCB1 Suppresses Growth and Shows Additive Effects With Gemcitabine in Pancreatic Ductal Adenocarcinoma via the Unfolded Protein Response.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Qiang Hua,Ke Zhang,Jie Sheng,Zhou-Yu Ning,Ye Li,Wei-Dong Shi,Lu-Ming Liu
View all publications
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Product promise

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