Rabbit Polyclonal NUCB1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUCB1 aa 200 to C-terminus.
Images
Publications
Filter by
- Computational and structural biotechnology journal 21:1510-15222023Identification of and as ovarian endometriosis risk-associated genes using integrative genomic analyses and functional experiments.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYizhou Huang,Jie Luo,Yue Zhang,Tao Zhang,Xiangwei Fei,Liqing Chen,Yingfan Zhu,Songyue Li,Caiyun Zhou,Kaihong Xu,Yunlong Ma,Jun Lin,Jianhong ZhouPubMed 36851918
- Frontiers in cell and developmental biology 9:6418362021NUCB1 Suppresses Growth and Shows Additive Effects With Gemcitabine in Pancreatic Ductal Adenocarcinoma via the Unfolded Protein Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong-Qiang Hua,Ke Zhang,Jie Sheng,Zhou-Yu Ning,Ye Li,Wei-Dong Shi,Lu-Ming LiuPubMed 33855021
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NUCB1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q02818
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Major calcium-binding protein of the Golgi which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates alpha subunits of guanine nucleotide-binding proteins (G proteins) (By similarity).
Alternative names
NUC, NUCB1, Nucleobindin-1, CALNUC
Recommended products
Rabbit Polyclonal NUCB1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUCB1 aa 200 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NUCB1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q02818
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein NUCB1 also known as Nucleobindin-1 functions mechanically as a calcium-binding protein. It has approximately a mass of 55 kDa and belongs to a group of calcium-binding proteins containing EF-hand motifs. NUCB1 expresses in a variety of tissues notably the nervous system and endocrine tissues. The protein comprises several functional domains including a signal peptide helix-loop-helix motif and nuclear localization signal.
- Biological function summary
NUCB1 participates in the regulation of cellular calcium homeostasis and signaling pathways. It is not a part of a multi-protein complex but it acts as a molecular chaperone influencing the stability and function of other proteins within the cell. NUCB1 also plays a role in secretory pathways within the cell which is relevant to its expression in endocrine tissues. Its regulatory functions in calcium signaling are critical for cellular activities such as secretion and cell division.
- Pathways
NUCB1 interacts with cellular signaling mechanisms particularly those related to intracellular calcium dynamics. It is involved in pathways like the calcium signaling pathway and the insulin signaling pathway. Within these pathways NUCB1 associates with proteins such as calmodulin which also binds calcium and contributes to signal transduction. The protein modulates activities within these pathways affecting how cells respond to external signals.
- Associated diseases and disorders
NUCB1 exhibits associations with certain conditions linked to disturbed calcium regulation. One such disorder is Alzheimer's disease where alterations in calcium signaling play a role in disease progression. The protein furthermore relates to type 2 diabetes involving its role in the insulin signaling pathway. In these contexts NUCB1 connects to other proteins such as amyloid precursor protein in Alzheimer's disease highlighting disruptions in calcium homeostasis mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-NUCB1 antibody (ab154262)
10% SDS PAGE
All lanes: Western blot - Anti-NUCB1 antibody (ab154262) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 54 kDa
Western blot - Anti-NUCB1 antibody (ab154262)
10% SDS PAGE
All lanes: Western blot - Anti-NUCB1 antibody (ab154262) at 1/1000 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 54 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB1 antibody (ab154262)
Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue labeling NUCB1 with ab154262 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-NUCB1 antibody (ab154262)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling NUCB1 with ab154262 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com