AB224348

Anti-NUCB2 antibody

Name: Anti-NUCB2 antibody (ab224348) | Abcam
Brand: Abcam Limited
SKU: AB224348
Price: 645 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal NUCB2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUCB2 aa 300 to C-terminus.

View Alternative Names

NEFA, NUCB2, Nucleobindin-2, DNA-binding protein NEFA, Epididymis secretory protein Li 109, Gastric cancer antigen Zg4, Prepronesfatin

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-NUCB2 antibody (AB224348)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUCB2 antibody (AB224348)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for NUCB2 (green) using ab224348 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB2 antibody (AB224348)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB2 antibody (AB224348)

Paraffin-embedded human stomach tissue stained for NUCB2 using ab224348 at 1/1000 in immunohistochemical analysis.

Supplier Data

Western blot - Anti-NUCB2 antibody (AB224348)

All lanes:

Western blot - Anti-NUCB2 antibody (ab224348) at 1/100 dilution

All lanes:

HL-60 (human promyelocytic leukemia cell line) cell lysate

Predicted band size: 50 kDa

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Supplier Data

Western blot - Anti-NUCB2 antibody (AB224348)

All lanes:

Western blot - Anti-NUCB2 antibody (ab224348) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 50 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human NUCB2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P80303

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUCB2 also known as nesfatin-1 precursor is a calcium-binding protein involved in various physiological processes. This 50-kDa protein integrates signals related to energy balance and is important for appetite regulation. NUCB2 is expressed in several tissues such as the hypothalamus pancreas and adipose tissue which suggests its involvement in widespread bodily functions. The protein is produced as an 82-kDa precursor protein cleaved to release active fragments like nesfatin-1 which possesses biological activity.

Biological function summary

This protein influences food intake and energy homeostasis. NUCB2 is part of a signaling complex that modulates cellular responses to metabolic needs. It functions as a satiety molecule signaling when the body does not require additional food intake. The protein's regulatory role in appetite makes it important for maintaining body weight and energy utilization. NUCB2’s expression in specific brain areas particularly indicates its role in central nervous system pathways that control energy use.

Pathways

NUCB2 engages in vital neural circuits governing appetite and energy expenditure. It connects with the melanocortin and neuropeptide Y pathways which are central to maintaining energy balance and body weight. The interaction of NUCB2 with neuropeptide Y an essential regulator of hunger exemplifies how it modulates these pathways despite its main role not being in leptin signaling.

Disturbances in NUCB2 are linked to obesity and stress-related conditions. Obesity involves disrupted signaling where NUCB2’s role as a satiety signal becomes critical. The protein also plays a part in stress-related pathways influencing stress-induced appetite changes which can relate to conditions like depression. Alterations in NUCB2 expression or function can lead to abnormal body weight and stress responses making it a potential target for therapeutic interventions in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Calcium-binding protein which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3 (By similarity).. Nesfatin-1. Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance.

See full target information NUCB2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology :18676 PubMed37997936

2023

WITHDRAWN: Ethyl acetate extracted from the chinese medicinal plant Caesalpinia sappan L alleviates gustatory response in diabetes mellitus rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Cao,Li Cui,Wen Yang

Oncology letters 19:3653-3664 PubMed32391090

2020

Nucleobindin-2 enhances the epithelial-mesenchymal transition in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Tao,Wen-Bin Niu,Peng-Hui Dou,Shao-Bin Ni,Yi-Peng Yu,Li-Cheng Cai,Xin-Yuan Wang,Shu-Yi Li,Cheng Zhang,Zhen-Guo Luo

OncoTargets and therapy 12:1309-1318 PubMed30863097

2019

High expression of NUCB2 promotes papillary thyroid cancer cells proliferation and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Jingzhu Zhao,Xinwei Yun,Xianhui Ruan,Jiadong Chi,Yang Yu,Yigong Li,Xiangqian Zheng,Ming Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NUCB2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NUCB2 antibody (AB224348)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB2 antibody (AB224348)

Western blot - Anti-NUCB2 antibody (AB224348)

Western blot - Anti-NUCB2 antibody (AB224348)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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