AB229683

Anti-NUCB2 antibody - C-terminal

Name: Anti-NUCB2 antibody - C-terminal (ab229683) | Abcam
Brand: Abcam Limited
SKU: AB229683

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(4 Publications)

Rabbit Polyclonal NUCB2 antibody. C-terminal. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUCB2.

View Alternative Names

NEFA, NUCB2, Nucleobindin-2, DNA-binding protein NEFA, Epididymis secretory protein Li 109, Gastric cancer antigen Zg4, Prepronesfatin

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NUCB2 antibody - C-terminal (AB229683)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUCB2 antibody - C-terminal (AB229683)

4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for NUCB2 (green) using ab229683 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB2 antibody - C-terminal (AB229683)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB2 antibody - C-terminal (AB229683)

Paraffin-embedded rat brain tissue stained for NUCB2 using ab229683 at 1/500 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, IP, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NUCB2. The exact immunogen used to generate this antibody is proprietary information.

P80303

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUCB2 also known as nesfatin-1 precursor is a calcium-binding protein involved in various physiological processes. This 50-kDa protein integrates signals related to energy balance and is important for appetite regulation. NUCB2 is expressed in several tissues such as the hypothalamus pancreas and adipose tissue which suggests its involvement in widespread bodily functions. The protein is produced as an 82-kDa precursor protein cleaved to release active fragments like nesfatin-1 which possesses biological activity.

Biological function summary

This protein influences food intake and energy homeostasis. NUCB2 is part of a signaling complex that modulates cellular responses to metabolic needs. It functions as a satiety molecule signaling when the body does not require additional food intake. The protein's regulatory role in appetite makes it important for maintaining body weight and energy utilization. NUCB2’s expression in specific brain areas particularly indicates its role in central nervous system pathways that control energy use.

Pathways

NUCB2 engages in vital neural circuits governing appetite and energy expenditure. It connects with the melanocortin and neuropeptide Y pathways which are central to maintaining energy balance and body weight. The interaction of NUCB2 with neuropeptide Y an essential regulator of hunger exemplifies how it modulates these pathways despite its main role not being in leptin signaling.

Disturbances in NUCB2 are linked to obesity and stress-related conditions. Obesity involves disrupted signaling where NUCB2’s role as a satiety signal becomes critical. The protein also plays a part in stress-related pathways influencing stress-induced appetite changes which can relate to conditions like depression. Alterations in NUCB2 expression or function can lead to abnormal body weight and stress responses making it a potential target for therapeutic interventions in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-binding protein which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3 (By similarity).. Nesfatin-1. Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance. In intestinal epithelial cells, plays a role in the inhibition of hepatic glucose production via MC4R receptor leading to increased cyclic adenosine monophosphate (cAMP) levels and glucagon-like peptide 1 (GLP-1) secretion (PubMed : 39562740).

See full target information NUCB2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 44:54-74 PubMed39562740

2024

Intestinal NUCB2/nesfatin-1 regulates hepatic glucose production via the MC4R-cAMP-GLP-1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Geng,Shan Yang,Xuejiao Tang,Shiyao Xue,Ke Li,Dongfang Liu,Chen Chen,Zhiming Zhu,Hongting Zheng,Yuanqiang Wang,Gangyi Yang,Ling Li,Mengliu Yang

Cell death & disease 12:438 PubMed33935281

2021

ITIH5, a p53-responsive gene, inhibits the growth and metastasis of melanoma cells by downregulating the transcriptional activity of KLF4.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Liu,Feng Cao,Xiaojie Li,Li Zhang,Zhengrong Liu,Xiaodong Li,Jingrong Lin,Chuanchun Han

Cancer biotherapy & radiopharmaceuticals 34:581-588 PubMed31697592

2019

Nucleobindin-2 Promotes the Growth and Invasion of Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Jun Liu,Jia-Xi Lv,Jun Liu,Xue-Bin Zhang,Lei-Bo Wang

Journal of experimental & clinical cancer research 37:176 PubMed30055641

2018

Regulation of the adaptation to ER stress by KLF4 facilitates melanoma cell metastasis via upregulating NUCB2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Zhang,Jingrong Lin,Yulin Chao,Lu Zhang,Lei Jin,Na Li,Ruiping He,Binbin Ma,Wenzhi Zhao,Chuanchun Han

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUCB2 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-NUCB2 antibody - C-terminal (AB229683)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB2 antibody - C-terminal (AB229683)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise