AB215396

Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker

Name: Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker (ab215396) | Abcam
Brand: Abcam Limited
SKU: AB215396
Rating: 5 (1 reviews)

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(1 Review)

(4 Publications)

Mouse Monoclonal Human Nuclear Antigen antibody. Nucleus marker. Suitable for IHC-P, ICC/IF, Flow Cyt and reacts with Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Nuclei of HL60 cells.

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker (AB215396)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker (AB215396)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Human Nuclear Antigen with ab215396 at 0.5 μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker (AB215396)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker (AB215396)

Immunocytochemistry analysis of HeLa cells labelling Human Nuclear Antigen antibody with ab215396 at 2 μg/mL. Goat Anti-mouse IgG (Alexa Fluor® 488) was used as the secondary antibody (green). Membranes were labeled with Phalloidin (Red).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker (AB215396)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Human Nuclear Antigen with ab215396 at 0.5 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker (AB215396)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling Human Nuclear Antigen with ab215396 at 0.5 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker (AB215396)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat colon tissue labeling Human Nuclear Antigen with ab215396 at 0.5 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NM106

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Rat, Human

Applications

Flow Cyt, ICC/IF, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "(Primary incubation for 30 minutes at RT).Perform heat mediated antigen retrieval with 10mM Tris with 1mM EDTA, pH 9, for 45 min at 95°C followed by cooling at RT for 20 minutes before commencing with IHC staining protocol. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "(Primary incubation for 30 minutes at RT).Perform heat mediated antigen retrieval with 10mM Tris with 1mM EDTA, pH 9, for 45 min at 95°C followed by cooling at RT for 20 minutes before commencing with IHC staining protocol. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "" }, "Pig": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "(Primary incubation for 30 minutes at RT).Perform heat mediated antigen retrieval with 10mM Tris with 1mM EDTA, pH 9, for 45 min at 95°C followed by cooling at RT for 20 minutes before commencing with IHC staining protocol. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab215396 was purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nuclear Antigen often also referred to as the nuclear antigen (NA) or nuclear matrix protein performs important mechanical roles within the cell nucleus. The protein has an approximate mass of around 120 kDa and mostly localizes within the nuclear compartment of cells. Researchers have observed that Nuclear Antigen binds tightly to chromatin and plays a part in maintaining nuclear integrity and organization. This target shows expression in proliferative tissues and is linked with cell cycle regulation.

Biological function summary

Nuclear Antigen provides critical involvement in DNA replication and repair processes. It serves as a scaffold within the nucleus facilitating interactions between DNA and various nuclear enzymes. Nuclear Antigen acts as part of the nuclear matrix complex supporting cellular processes that maintain genomic stability. By modulating the structure of chromatin Nuclear Antigen influences gene expression and cell proliferation making it a noteworthy player in regulating cellular growth.

Pathways

The Nuclear Antigen influences cellular processes like cell cycle control and apoptosis. It interacts closely with pathways including the DNA damage response pathway and cell cycle checkpoints. Within these pathways its interaction with proteins such as p53 and BRCA1 is of particular importance. These interactions assist in the coordination of repair mechanisms during DNA damage events ensuring proper cell cycle progression and preventing genomic instability.

Studies link Nuclear Antigen to specific types of cancer and autoimmune diseases. Its association with cancer comes from its role in cell cycle regulation as its altered expression is seen in several tumor types. Conversely in autoimmune disorders Elevated nuclear antigen expression levels can elicit autoimmune reactions suggesting their significant role. Here the protein interacts with disease-related molecules such as oncogenes in cancer or autoantibodies in autoimmune conditions highlighting its contribution to disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Human Nuclear Antigen

Additional targets

Human Nuclei,Rat Nuclei

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 11:1213021 PubMed37675407

2023

Delivery of multipotent adult progenitor cells via a functionalized plasma polymerized surface accelerates healing of murine diabetic wounds.

Applications

Unspecified application

Species

Unspecified reactive species

S J Mills,G T Kirby,B R Hofma,L E Smith,P Statham,B Vaes,A E Ting,R Short,A J Cowin

Experimental and therapeutic medicine 22:1013 PubMed34373699

2021

Nerve growth factor enhances the therapeutic effect of mesenchymal stem cells on diabetic periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Li,Suyu Wang,Yihan Xiao,Bing Liu,Jianliang Pang

Molecular medicine reports 20:3065-3074 PubMed31432152

2019

Therapeutic effect of transplantation of human bone marrow‑derived mesenchymal stem cells on neuron regeneration in a rat model of middle cerebral artery occlusion.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Xie,Ming Deng,Qin-Guo Sun,Yong-Gang Ma,Yan Zhou,Jiang-Hua Ming,Qing Chen,Shi-Qing Liu,Jun-Qi Liu,Jun Cai,Fei Wu

Clinical cancer research : an official journal of the American Association for Cancer Research 20:6551-8 PubMed25294908

2014

Identification of recurrent FGFR3-TACC3 fusion oncogenes from lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Marzia Capelletti,Michael E Dodge,Dalia Ercan,Peter S Hammerman,Seung-Il Park,Jhingook Kim,Hidefumi Sasaki,David M Jablons,Doron Lipson,Lauren Young,Phil J Stephens,Vincent A Miller,Neal I Lindeman,Kiara J Munir,William G Richards,Pasi A Jänne

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker (AB215396)

Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker (AB215396)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker (AB215396)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker (AB215396)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker (AB215396)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (4)

Product promise