Rabbit Recombinant Monoclonal Nuclear Matrix Protein p84 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (AB232034), expandable thumbnail](https://content.abcam.com/products/images/nuclear-matrix-protein-p84-antibody-epr56622-bsa-and-azide-free-ab232034--immunocytochemistry-immunofluorescence-img63223.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (AB232034)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (AB232034), expandable thumbnail](https://content.abcam.com/products/images/nuclear-matrix-protein-p84-antibody-epr56622-bsa-and-azide-free-ab232034--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108348.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (AB232034)")
![Flow Cytometry (Intracellular) - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (AB232034), expandable thumbnail](https://content.abcam.com/products/images/nuclear-matrix-protein-p84-antibody-epr56622-bsa-and-azide-free-ab232034--flow-cytometry-intracellular-img50442.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (AB232034)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes (Heat to 98°C, allow to cool for 10-20 minutes) Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). Required for efficient export of polyadenylated RNA (PubMed:23222130). The THOC1-THOC2-THOC3 core complex alone is sufficient to bind export factor NXF1-NXT1 and promote ATPase activity of DDX39B/UAP56 (PubMed:33191911). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed:15833825, PubMed:15998806, PubMed:17190602). Regulates transcriptional elongation of a subset of genes (PubMed:22144908). Involved in genome stability by preventing co-transcriptional R-loop formation (By similarity). May play a role in hair cell formation, hence may be involved in hearing (By similarity). Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1. (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.
Alternative names
HPR1, THOC1, THO complex subunit 1, Nuclear matrix protein p84, hTREX84, p84N5
Recommended products
Rabbit Recombinant Monoclonal Nuclear Matrix Protein p84 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5662(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232034 is the carrier-free version of Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] ab131268.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nuclear Matrix Protein p84 also known as 5E10 or simply p84 is a structural protein of the nuclear matrix. It has an approximate mass of 84 kDa. Researchers have identified it as an integral component of the nucleoskeleton providing key structural support within the cell nucleus. The expression of p84 is resourceful within various cell types especially in tissues with high rates of cellular proliferation and turnover.
- Biological function summary
Nuclear Matrix Protein p84 assists in nuclear architecture maintenance and organization. It takes part in DNA replication processes and gene regulation by interacting with chromatin and transcription factors. Furthermore p84 contributes to the formation of the ribonucleoprotein complex facilitating RNA processing and transport. This involvement in multiple nuclear tasks positions it as a critical player in gene expression regulation mechanisms.
- Pathways
Nuclear Matrix Protein p84 influences vital cellular activities such as the cell cycle and DNA repair. Its connections are apparent in the regulation of the p53 signaling pathway where it interacts with several regulatory proteins to monitor cell cycle progression and stress responses. p84’s activity also aligns with the DNA damage response pathway highlighting its cooperation with checkpoint proteins like ATM and ATR that uphold genomic integrity during cellular stress conditions.
- Associated diseases and disorders
Aberrant Nuclear Matrix Protein p84 expression aligns with cancer and autoimmune conditions. In certain cancers deregulation of p84 can result in altered cell cycle control and resistance to apoptosis. Its interaction with p53 and other tumor suppressor proteins directly influences cancer cell survival and proliferation. Additionally altered p84 expression has connections to lupus specifically affecting the autoantigen properties and leading to immune system malfunctions. Understanding these connections helps scientists investigate therapeutic targets for associated diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (ab232034), expandable thumbnail](https://content.abcam.com/products/images/nuclear-matrix-protein-p84-antibody-epr56622-bsa-and-azide-free-ab232034--immunocytochemistry-immunofluorescence-img63223.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (ab232034)")
Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (ab232034)
Immunofluorescent staining of HeLa cells labelling Nuclear Matrix Protein p84 with Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] ab131268 at 1/100 dilution. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] ab131268).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (ab232034), expandable thumbnail](https://content.abcam.com/products/images/nuclear-matrix-protein-p84-antibody-epr56622-bsa-and-azide-free-ab232034--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108348.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (ab232034)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (ab232034)
Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue labeling Nuclear Matrix Protein p84 with Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] ab131268 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] ab131268).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (ab232034), expandable thumbnail](https://content.abcam.com/products/images/nuclear-matrix-protein-p84-antibody-epr56622-bsa-and-azide-free-ab232034--flow-cytometry-intracellular-img50442.jpg "Flow Cytometry (Intracellular) - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (ab232034)")
Flow Cytometry (Intracellular) - Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - BSA and Azide free (ab232034)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Nuclear Matrix Protein p84 with purified Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] ab131268 at 1/120 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] ab131268).
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