Anti-Nuclear Pore Complex Proteins antibody [Mab414]

• ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (AB24609)

ab24609 (1/500) staining Nuclear Pore Complex Proteins in human Hela Cells (green). Cells were fixed with Paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton-X100 at room T°C) or Methanol (6 min in Methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with Dapi in order to highligh the nucleus (blue).

This image was generated using the ascites version of the product.

Image and protocol kindly provided by Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (AB24609)

HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) mouse IgG1, κ isotype control (Negative) or (B-D) Nuclear Pore Complex (ab24609) overnight at 4°C followed by Alexa Fluor^® 647 (Red) Goat anti-Mouse IgG incubation for one hour at room temperature. The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/5. Concentrations for (A-B) is 5 μg/ml, (C) is 2 μg/ml and (D) is 0.5 μg/ml.

• ICC/IF

AbReview8139****

Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (AB24609)

ab24609 (1/200) staininig Nuclear Pore Complex Proteins in human RPE-1 cells (green). Cells were fixed in paraformaldehyde, permeabilised with Triton X100 and counterstained with DAPI in order to highlight the nucleus (blue). Please refer to abreview for further experimental details.

This image was generated using the ascites version of the product.

This image is courtesy of an Abreview submitted by Dr Kirk McManus

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (AB24609)

ab24609 staining Nuclear Pore Complex Proteins in Human chronic myelogenous leukemia cells from bone marrow cells. Cells were fixed with 4% paraformaldehyde in DPBS buffer for 20 mins and permeabilised with 0.1% Triton X-100 for 20 min at room temperature. Cells were blocked with 2% normal goat serum in DPBS with 1% BSA and washed with 0.1% Tween 20.

This image was generated using the ascites version of the product.

Image courtesy of Saram N J et al. J Biol Chem. 2011 Nov 11; 286(45): 38989–39001. doi: 10.1074/jbc.M111.297952

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (AB24609)

Immunofluorescence of HeLa with Nuclear Pore Complex Proteins Mouse primary antibody ab24609 at 1 µg/mL. Alexa Fluor^® 594 (Red) Goat anti-Mouse IgG was used as secondary antibody. Nuclei were counterstained with DAPI (Blue). Exposure time is 80 ms.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (AB24609)

IHC staining using ab24609 on formalin-fixed paraffin-embedded human tonsiltissue. The tissue was incubated with 1 μg/mL of ab24609 overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor^® 647 goat anti-mouse IgG antibody (red) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong^™ Gold Antifade Mounting Solution. The image was captured with a 40x objective. Scalebar = 50 μM.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (AB24609)

ab24609 (1/500) staining Nuclear Pore Complex Proteins in murine NIH/3T3 Cells (green). Cells were fixed with Paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton-X100 at room T°C) or Methanol (6 min in Methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with Dapi in order to highligh the nucleus (blue).

This image was generated using the ascites version of the product.

Image and protocol kindly provided by Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome

• WB

Supplier Data

Western blot - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (AB24609)

All lanes:

Western blot - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (ab24609)

Lane 1:

HaCaT (Human keratinocyte cell line) cell lysate

Lane 2:

Daudi (Human Burkitt's lymphoma cell line) cell lysate

Lane 3:

RAW 264.7 (Mouse) cell lysate

Lane 4:

UMR106 (Rat) cell lysate

Secondary

All lanes:

HRP Goat anti-Mouse secondary antibody

Predicted band size: 62 kDa

false