Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (AB2734)

Immunohistochemistry was performed on normal biopsies of deparaffinized Rat lymph node tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (AB2734)

Immunohistochemistry was performed on normal biopsies of deparaffinized Rat kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (AB2734)

Immunohistochemistry was performed on normal biopsies of deparaffinized Rat brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• WB

Supplier Data

Western blot - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (AB2734)

* an uncharacterized band at ~45 kDa.

All lanes:

Western blot - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734) at 1/1000 dilution

Lane 1:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 30 µg

Lane 2:

THP-1 (Human monocytic leukemia cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 30 µg

Lane 4:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

false