Skip to main content

Rabbit Polyclonal Nuclear Receptor Corepressor NCoR antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 20 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody (AB3482), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody (AB3482), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody (AB3482), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody (AB3482), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information. Database link Q60974

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPChIPWBIHC-PICC/IF
Human
Expected
Expected
Expected
Tested
Tested
Mouse
Expected
Expected
Expected
Tested
Expected
Rat
Predicted
Predicted
Predicted
Predicted
Predicted

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

-

Notes

Pubmed ID: 16930818.

Species

Human

Dilution info

-

Notes

Pubmed ID: 16930818.

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

-

Notes

By Western blot, this antibody detects a protein at ~270 kDa representing N-CoR from HeLa cell extracts and mouse RAW 264.7/primary macrophages.

Species

Human

Dilution info

-

Notes

By Western blot, this antibody detects a protein at ~270 kDa representing N-CoR from HeLa cell extracts and mouse RAW 264.7/primary macrophages.

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/200.00000 - 1/1000.00000

Notes

-

Species

Human

Dilution info

1/200.00000 - 1/1000.00000

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/500.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. Participates in the transcriptional repressor activity produced by BCL6. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation (By similarity). Mediates the NR1D1-dependent repression and circadian regulation of TSHB expression (PubMed:24794873). The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene ARTNL/BMAL1 and the genes involved in lipid metabolism in the liver (PubMed:19037247).

Alternative names

Recommended products

Rabbit Polyclonal Nuclear Receptor Corepressor NCoR antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 20 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information. Database link Q60974
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Nuclear Receptor Corepressor (NCoR) also known as NCoR1 or simply N-CoR is a significant modulator of gene transcription. Mechanically NCoR functions by associating with nuclear receptors to repress transcription. It is a part of a large protein complex with a molecular weight roughly around 270 kDa. Researchers find NCoR expressed in various tissues throughout the human body with especially high expression levels in the liver and central nervous system. The NCoR protein plays an important role in regulating transcriptional repression through its interaction with other corepressors and nuclear receptors.

Biological function summary

NCoR engages with other proteins to maintain transcriptional repression adapting to cellular signaling. It is part of a protein complex known as the NCoR complex that includes histone deacetylases and transcription factors. The corepressors have significant roles in modulating gene expression critical for multiple cellular and physiological processes. The NCoR complex fine-tunes the balance between the activation and repression of target genes influencing developmental metabolic and immune responses.

Pathways

NCoR participates in thyroid hormone signaling and retinoic acid receptor pathways both critical to maintaining homeostasis. Its association with proteins like histone deacetylases enables NCoR to alter chromatin structure and suppress gene transcription. In these pathways NCoR serves as a bridge connecting various nuclear receptors to other corepressors and transcriptional machinery ensuring precise regulation of gene expression involved in growth differentiation and metabolism.

Associated diseases and disorders

NCoR is linked to cancer and metabolic syndromes. Aberrations in the NCoR function or expression have associations with breast cancer affecting genes that control cell proliferation and apoptosis. Additionally in metabolic diseases such as obesity altered NCoR activity impacts genes involved in energy balance and insulin signaling. Related proteins like the silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) also associate with these disorders emphasizing NCoR's critical role in maintaining normal biological functions and preventing disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody (ab3482), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR in the nucleus of Mouse breast tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissue was blocked in 3% H2O2-methanol for 15 min at room temperature, then incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody (ab3482), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR in the nucleus of Human breast carcinoma (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissue was blocked in 3% H2O2-methanol for 15 min at room temperature, then incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody (ab3482), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR (green) in the nucleus and cytoplasm of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 °C. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody (ab3482), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR (green) in the nucleus and cytoplasm of HeLa cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 °C. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com