Anti-Nuclear Receptor Corepressor NCoR antibody [EPR23834-46]

• IP

Supplier Data

Immunoprecipitation - Anti-Nuclear Receptor Corepressor NCoR antibody [EPR23834-46] (AB300615)

Nuclear Receptor Corepressor NCoR was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug with ab300615 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300615 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug
Lane 2 : ab300615 IP in NIH/3T3 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300615 in NIH/3T3 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

Lysate was freshly made and used for immunoprecipitation immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-Nuclear Receptor Corepressor NCoR antibody [EPR23834-46] (ab300615) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 μg

Lane 2:

NIH/3T3 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 300 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Nuclear Receptor Corepressor NCoR antibody [EPR23834-46] (AB300615)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Nuclear Receptor Corepressor NCoR antibody [EPR23834-46] (ab300615) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

Jurkat transfected with siRNA 1 specifically targeting NCOR1, whole cell lysate at 20 µg

Lane 3:

Jurkat transfected with siRNA 2 specifically targeti NCOR1, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 300 kDa

true

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-Nuclear Receptor Corepressor NCoR antibody [EPR23834-46] (AB300615)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTLow expression : HCT 116. SW480 and HCT 116 lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 70 seconds

All lanes:

Western blot - Anti-Nuclear Receptor Corepressor NCoR antibody [EPR23834-46] (ab300615) at 1/1000 dilution

Lane 1:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 20 μg

Lane 2:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 300 kDa

false

Exposure time: 70s

• WB

Supplier Data

Western blot - Anti-Nuclear Receptor Corepressor NCoR antibody [EPR23834-46] (AB300615)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTLysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 26 seconds

All lanes:

Western blot - Anti-Nuclear Receptor Corepressor NCoR antibody [EPR23834-46] (ab300615) at 1/1000 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 300 kDa

false

Exposure time: 26s