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Rabbit Recombinant Monoclonal Nuclear Receptor Corepressor NCoR antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
ChIP | IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
---|---|---|---|---|---|---|
Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
Mouse | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Tested |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Mediates transcriptional repression by certain nuclear receptors (PubMed:20812024). Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. Participates in the transcriptional repressor activity produced by BCL6. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Mediates the NR1D1-dependent repression and circadian regulation of TSHB expression (By similarity). The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene ARTNL/BMAL1 and the genes involved in lipid metabolism in the liver (By similarity).
Nuclear receptor corepressor 1, N-CoR, N-CoR1, NCOR1, KIAA1047
Rabbit Recombinant Monoclonal Nuclear Receptor Corepressor NCoR antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human samples.
Nuclear receptor corepressor 1, N-CoR, N-CoR1, NCOR1, KIAA1047
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR23834-46
Affinity purification Protein A
Blue Ice
+4°C
Do Not Freeze
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
NCoR engages with other proteins to maintain transcriptional repression adapting to cellular signaling. It is part of a protein complex known as the NCoR complex that includes histone deacetylases and transcription factors. The corepressors have significant roles in modulating gene expression critical for multiple cellular and physiological processes. The NCoR complex fine-tunes the balance between the activation and repression of target genes influencing developmental metabolic and immune responses.
The Nuclear Receptor Corepressor (NCoR) also known as NCoR1 or simply N-CoR is a significant modulator of gene transcription. Mechanically NCoR functions by associating with nuclear receptors to repress transcription. It is a part of a large protein complex with a molecular weight roughly around 270 kDa. Researchers find NCoR expressed in various tissues throughout the human body with especially high expression levels in the liver and central nervous system. The NCoR protein plays an important role in regulating transcriptional repression through its interaction with other corepressors and nuclear receptors.
NCoR participates in thyroid hormone signaling and retinoic acid receptor pathways both critical to maintaining homeostasis. Its association with proteins like histone deacetylases enables NCoR to alter chromatin structure and suppress gene transcription. In these pathways NCoR serves as a bridge connecting various nuclear receptors to other corepressors and transcriptional machinery ensuring precise regulation of gene expression involved in growth differentiation and metabolism.
NCoR is linked to cancer and metabolic syndromes. Aberrations in the NCoR function or expression have associations with breast cancer affecting genes that control cell proliferation and apoptosis. Additionally in metabolic diseases such as obesity altered NCoR activity impacts genes involved in energy balance and insulin signaling. Related proteins like the silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) also associate with these disorders emphasizing NCoR's critical role in maintaining normal biological functions and preventing disease.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using AB300615, the same antibody clone in a different buffer formulation.
Nuclear Receptor Corepressor NCoR was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug with AB300615 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using AB300615 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(AB131366) was used at 1/5000 dilution. Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug
Lane 2: ABAB300615 IP in NIH/3T3 whole cell lysate
Lane 3:RABbit monoclonal IgG (AB172730) instead of AB300615 in NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
Lysate was freshly made and used for immunoprecipitation immediately to minimize protein degradation.
All lanes: Immunoprecipitation - Anti-Nuclear Receptor Corepressor NCoR antibody [EPR23834-46] (AB300615) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 μg
Lane 2: NIH/3T3 whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366) at 1/5000 dilution
Observed band size: 300 kDa
Exposure time: 3min
Nuclear Receptor Corepressor NCoR was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug with AB300615 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using AB300615 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(AB131366) was used at 1/5000 dilution. Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug
Lane 2: ABAB300615 IP in NIH/3T3 whole cell lysate
Lane 3:RABbit monoclonal IgG (AB172730) instead of AB300615 in NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
Lysate was freshly made and used for immunoprecipitation immediately to minimize protein degradation.
This data was developed using 300615, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Low expression: HCT 116.
SW480 and HCT 116 lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
70 seconds
Exposure time:
All lanes: Western blot - Anti-NCOR1 antibody [EPR23834-46] (AB300615) at 1/1000 dilution
Lane 1: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 2: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate 20 μg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Observed band size: 300 kDa
Exposure time: 70s
Blocking and diluting buffer and concentration: 5% NFDM/TBSTLow expression: HCT 116.
SW480 and HCT 116 lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Exposure time: 70 seconds
This data was developed using 300615, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
26 seconds
Exposure time:
All lanes: Western blot - Anti-NCOR1 antibody [EPR23834-46] (AB300615) at 1/1000 dilution
All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 μg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Observed band size: 300 kDa
Exposure time: 26s
Blocking and diluting buffer and concentration: 5% NFDM/TBSTLysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Exposure time: 26 seconds
This data was developed using 300615, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: This blot was developed using a high sensitivity ECL substrate.
15 seconds
Exposure time:
All lanes: Western blot - Anti-NCOR1 antibody [EPR23834-46] (AB300615) at 1/1000 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) transfected with scrambled siRNA control, whole cell lysate 20 μg
Lane 2: Jurkat transfected with siRNA 1 specifically targeti NCOR1, whole cell lysate 20 μg
Lane 3: Jurkat transfected with siRNA 2 specifically targeti NCOR1, whole cell lysate 20 μg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 300 kDa
Exposure time: 15s
Blocking and diluting buffer and concentration: 5% NFDM/TBSTThis blot was developed using a high sensitivity ECL substrate.
Exposure time: 15 seconds
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