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AB254080

Anti-Nuclei antibody [235-1]

5

(1 Review)

|

(2 Publications)

Mouse Monoclonal Human Nuclei antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Human Nuclei protein.
1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nuclei antibody [235-1] (AB254080)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nuclei antibody [235-1] (AB254080)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Nuclei using ab254080 at 1/100 dilution in ICC/IF (green).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

235-1

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Specific for endogenous levels of human nuclei. Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100.</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100.</p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100.</p>" } } }
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Product details

This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The nuclei serve as the command center of eukaryotic cells housing the genetic material in the form of DNA and its associated proteins. Commonly known as the cell's "control center" the nucleus typically weighs in at approximately 1.8 x 10^12 Daltons when considering a moderate-sized nucleus though exact mass can vary based on cell type. Nuclei reside within the cytoplasm of cells delineated by the double-layered nuclear envelope. Within it chromatin (which comprises DNA wrapped around histone proteins) and the nucleolus (site of ribosomal RNA synthesis) are key components.
Biological function summary

The nucleus plays a central role in governing cell activities through the regulation of gene expression. It controls transcription processes and executes RNA processing tasks. It is part of several macromolecular complexes including chromatin-remodeling complexes and splicing machinery. These complexes are important for efficient transcriptional regulation and RNA maturation enabling precise cellular responses to environmental and developmental cues.

Pathways

Nuclei integrate information from signaling pathways to modulate gene expression. They are vital components of pathways such as the cell cycle regulation pathway and the DNA damage response. They coordinate with proteins like cyclins and cyclin-dependent kinases (CDKs) in cell cycle progression and with proteins such as p53 and BRCA1/2 to repair DNA damage and prevent genomic instability. These pathways ensure proper cell division and stability of genetic information across generations.

Dysfunction in the nuclei can have serious implications for human health including cancer and neurodegenerative conditions. Mutations or dysregulations in nuclear proteins and DNA repair mechanisms such as those involving p53 or lamin proteins can lead to genomic instability and the development of various cancers. In neurodegenerative disorders nuclear abnormalities affecting RNA transport and processing may contribute to disease pathology highlighting the importance of nuclear integrity in maintaining neuronal function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Human Nuclei
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Neuro-oncology 25:2044-2057 PubMed37246765

2023

Ex vivo to in vivo model of malignant peripheral nerve sheath tumors for precision oncology.

Applications

Unspecified application

Species

Unspecified reactive species

Alex T Larsson,Himanshi Bhatia,Ana Calizo,Kai Pollard,Xiaochun Zhang,Eric Conniff,Justin F Tibbitts,Elizabeth Rono,Katherine Cummins,Sara H Osum,Kyle B Williams,Alexandra L Crampton,Tyler Jubenville,Daniel Schefer,Kuangying Yang,Yang Lyu,James C Pino,Jessica Bade,John M Gross,Alla Lisok,Carina A Dehner,John S A Chrisinger,Kevin He,Sara J C Gosline,Christine A Pratilas,David A Largaespada,David K Wood,Angela C Hirbe

Neural regeneration research 16:727-733 PubMed33063735

2020

Abnormal Glu/mGluR/PI3K pathway in the hippocampal neurovascular unit leads to diabetes-related depression.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Liu,Yuan-Shan Han,Lin Liu,Lin Tang,Hui Yang,Pan Meng,Hong-Qing Zhao,Yu-Hong Wang
View all publications
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