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AB72741

Anti-Nucleolar protein 14 antibody

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(1 Publication)

Mouse Polyclonal Nucleolar protein 14 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NOP14.

View Alternative Names

C4orf9, NOL14, RES4-25, NOP14, Nucleolar protein 14, Nucleolar complex protein 14

2 Images
Western blot - Anti-Nucleolar protein 14 antibody (AB72741)
  • WB

Unknown

Western blot - Anti-Nucleolar protein 14 antibody (AB72741)

All lanes:

Western blot - Anti-Nucleolar protein 14 antibody (ab72741) at 1/500 dilution

Lane 1:

Nucleolar protein 14 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 98 kDa

Observed band size: 105 kDa

false

Western blot - Anti-Nucleolar protein 14 antibody (AB72741)
  • WB

Unknown

Western blot - Anti-Nucleolar protein 14 antibody (AB72741)

All lanes:

Western blot - Anti-Nucleolar protein 14 antibody (ab72741) at 1/500 dilution

All lanes:

human pancreas tissue lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 98 kDa

Observed band size: 89 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NOP14.

P78316

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p>Predicted molecular weight for isoform 2: 91 kDa.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Nucleolar protein 14 (NOP14) also known as Cirhin or BCDS is a protein with a mass of approximately 90 kDa. It functions mechanically as an important component of ribosome biogenesis specifically during the early stages of 90S pre-ribosomal particle assembly. NOP14 is expressed largely within the nucleolus a sub-nuclear structure that is actively engaged in ribosomal RNA synthesis and ribosome assembly. Additionally NOP14 interacts with other nucleolar proteins and participates actively in the maturation of small subunit ribosomal RNA.
Biological function summary

NOP14 plays a significant role in the assembly of ribosomal subunits forming part of the small subunit (SSU) processome complex. This complex is vital for the processing of the 35S pre-rRNA into the 18S rRNA an important step in the formation of the 40S small ribosomal subunit. The SSU processome involves various proteins and small nucleolar RNAs emphasizing NOP14’s significance in both the structure and function within this large nucleolar complex. The integrity of ribosome production relies heavily on the precise actions of NOP14 within this multifaceted process.

Pathways

NOP14 participates in ribosome biogenesis a critical cellular pathway affecting protein synthesis. It involves significant interactions with the U3 small nucleolar RNP and other ribosome-associated proteins. Another essential pathway that NOP14 impacts is the translation initiation pathway where ribosome assembly begins conjoined efforts with other proteins like NOP56 and NOP58 influencing eukaryotic cell growth and division. These interactions highlight NOP14's interconnected role within cellular proliferation and growth regulation.

NOP14 is linked to North American Indian childhood cirrhosis (NAIC) a hepatological disorder. Mutations within the CIRH1A gene which encodes NOP14 disrupt normal ribosomal assembly leading to hepatic dysfunction. Additionally NOP14 associates with disorders related to ribosomopathies where defects in ribosomal function lead to diverse developmental anomalies and pathological conditions. An understanding of NOP14’s interaction with proteins like RPS19 in these contexts can provide insights into therapeutic targets for these ribosome-associated disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm (By similarity).
See full target information NOP14
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 9:32 PubMed38351062

2024

Docking protein 6 (DOK6) selectively docks the neurotrophic signaling transduction to restrain peripheral neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Guo,Pan Xiang,Xiaojiao Sun,Wei Liu,Jiafeng Zhou,Bin Yin,Lin Hou,Boqin Qiang,Huiliang Li,Pengcheng Shu,Xiaozhong Peng
View all publications
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Product promise

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