AB134164

Anti-Nucleolin antibody [EPR7951]

RabMAb
Recombinant
KO Validated
What is this?

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Recombinant Monoclonal Nucleolin antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

Nucleolin, Protein C23, NCL

3 Images

Lab

Western blot - Anti-Nucleolin antibody [EPR7951] (AB134164)

Lanes 1 - 3 : Merged signal (red and green). Green - ab134164 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab134164 was shown to specifically react with Nucleolin in wild-type HeLa cells as signal was lost in Nucleolin knockout cells. Wild-type and Nucleolin knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab134164 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Nucleolin antibody [EPR7951] (ab134164) at 1/10000 dilution

Lane 1:

Wild-type HeLa whole cell lysate at 20 µg

Lane 2:

Nucleolin knockout HeLa whole cell lysate at 20 µg

Lane 3:

MCF7 whole cell lysate at 20 µg

Predicted band size: 76 kDa

Observed band size: 100 kDa

false

Unknown

Western blot - Anti-Nucleolin antibody [EPR7951] (AB134164)

All lanes:

Western blot - Anti-Nucleolin antibody [EPR7951] (ab134164) at 1/1000 dilution

Lane 1:

K562 cell lysates at 10 µg

Lane 2:

HuT-78 cell lysates at 10 µg

Lane 3:

HeLa cell lysates at 10 µg

Lane 4:

Jurkat cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 76 kDa

false

OI-RD Scanning - Anti-Nucleolin antibody [EPR7951] (AB134164)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Nucleolin antibody [EPR7951] (AB134164)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-Nucleolin antibody [EPR7951] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7951

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleolin also known as C23 is a multifunctional protein mainly found in the nucleolus with a molecular weight of approximately 100 kDa. It plays a mechanical role in ribosomal RNA (rRNA) synthesis and ribosome biogenesis. Nucleolin is widely expressed in proliferating cells including HeLa culture and shows higher expression in cancer cells compared to normal cells. The nucleolin protein is involved in various nucleolar processes like the modulation of chromatin structure and nucleocytoplasmic transport.

Biological function summary

The nucleolin molecular weight protein contributes to regulating cellular processes such as chromatin remodeling rRNA transcription and mRNA stability. Nucleolin often forms complexes with various other proteins and nucleic acids enhancing its functionality within the cell nucleus. Its participation in processes like DNA replication and cell proliferation shows the central role nucleolin plays at the cellular level. It influences cellular responses by binding directly to specific RNA or DNA sequences.

Pathways

The nucleolin protein engages in major cellular pathways such as the RNA processing and p53 signaling pathways. Through the RNA processing pathway it interacts with proteins like fibrillarin to assist in rRNA maturation. Moreover within the p53 signaling pathway nucleolin regulates the activity of the tumor suppressor protein p53 impacting cell cycle progression and apoptosis. These interactions highlight nucleolin's role in maintaining cellular homeostasis and response to stress.

The nucleolin protein often shows altered expression in cancers and cardiovascular diseases. In cancer nucleolin's high expression levels correlate with tumor progression and metastasis making it a potential target for cancer therapies. In cardiovascular diseases nucleolin interacts with proteins like VEGF to influence angiogenesis which affects disease progression. Understanding nucleolin's involvement in these diseases provides insight into potential therapeutic targets and interventions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

See full target information NCL

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 52:8534-8551 PubMed38801080

2024

Parsing the roles of DExD-box proteins DDX39A and DDX39B in alternative RNA splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Shefali Banerjee,Chloe K Nagasawa,Steven G Widen,Mariano A Garcia-Blanco

Frontiers in molecular biosciences 10:1204124 PubMed37325470

2023

Long non-coding RNA lncMGC mediates the expression of TGF-β-induced genes in renal cells via nucleosome remodelers.

Applications

Unspecified application

Species

Unspecified reactive species

Mitsuo Kato,Zhuo Chen,Sadhan Das,Xiwei Wu,Jinhui Wang,Arthur Li,Wei Chen,Walter Tsark,Ragadeepthi Tunduguru,Linda Lanting,Mei Wang,Roger Moore,Markus Kalkum,Maryam Abdollahi,Rama Natarajan

Nucleic acid therapeutics 32:280-299 PubMed35852833

2022

NAT10 and DDX21 Proteins Interact with RNase H1 and Affect the Performance of Phosphorothioate Oligonucleotides.

Applications

Unspecified application

Species

Unspecified reactive species

Lingdi Zhang,Karla D Bernardo,Timothy A Vickers,Jun Tian,Xue-Hai Liang,Stanley T Crooke

International journal of medical sciences 16:212-220 PubMed30745801

2019

MicroRNA-29a is a key regulon that regulates BRD4 and mitigates liver fibrosis in mice by inhibiting hepatic stellate cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Hsien Huang,Hsing-Chun Kuo,Ya-Ling Yang,Feng-Sheng Wang

Oncotarget 7:86713-86729 PubMed27880931

2016

An XIST-related small RNA regulates KRAS G-quadruplex formation beyond X-inactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuli C Chang,Chien-Chih Chiu,Chung-Yee Yuo,Wen-Ling Chan,Ya-Sian Chang,Wen-Hsin Chang,Shou-Mei Wu,Han-Lin Chou,Ta-Chih Liu,Chi-Yu Lu,Wen-Kuang Yang,Jan-Gowth Chang

RNA (New York, N.Y.) 22:1760-1770 PubMed27659051

2016

ARiBo pull-down for riboproteomic studies based on label-free quantitative mass spectrometry.

Applications

Species

Human

Geneviève Di Tomasso,Lisa M Miller Jenkins,Pascale Legault

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nucleolin antibody [EPR7951]

Western blot - Anti-Nucleolin antibody [EPR7951] (AB134164)

Western blot - Anti-Nucleolin antibody [EPR7951] (AB134164)

OI-RD Scanning - Anti-Nucleolin antibody [EPR7951] (AB134164)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise