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Rabbit Recombinant Monoclonal Nucleolin antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (AB248709), expandable thumbnail
  • Western blot - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (AB248709), expandable thumbnail
  • OI-RD Scanning - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (AB248709), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Not recommended
Tested
Not recommended
Not recommended
Mouse
Not recommended
Predicted
Not recommended
Not recommended
Rat
Not recommended
Predicted
Not recommended
Not recommended

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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    1 products for Alternative Version

      Target data

      Function

      Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

      Alternative names

      Nucleolin, Protein C23, NCL

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      Rabbit Recombinant Monoclonal Nucleolin antibody. Carrier free. Suitable for WB and reacts with Human samples.

      Alternative names

      Nucleolin, Protein C23, NCL

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Carrier free

      Yes

      Clone number

      EPR7951

      Purification technique

      Affinity purification Protein A

      Dissociation constant

      2.9 x 10-11 M

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      +4°C

      Storage information

      Do Not Freeze

      Notes

      ab248709 is the carrier-free version of Anti-Nucleolin antibody [EPR7951] ab134164.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      Supplementary info

      Activity summary

      Nucleolin also known as C23 is a multifunctional protein mainly found in the nucleolus with a molecular weight of approximately 100 kDa. It plays a mechanical role in ribosomal RNA (rRNA) synthesis and ribosome biogenesis. Nucleolin is widely expressed in proliferating cells including HeLa culture and shows higher expression in cancer cells compared to normal cells. The nucleolin protein is involved in various nucleolar processes like the modulation of chromatin structure and nucleocytoplasmic transport.

      Biological function summary

      The nucleolin molecular weight protein contributes to regulating cellular processes such as chromatin remodeling rRNA transcription and mRNA stability. Nucleolin often forms complexes with various other proteins and nucleic acids enhancing its functionality within the cell nucleus. Its participation in processes like DNA replication and cell proliferation shows the central role nucleolin plays at the cellular level. It influences cellular responses by binding directly to specific RNA or DNA sequences.

      Pathways

      The nucleolin protein engages in major cellular pathways such as the RNA processing and p53 signaling pathways. Through the RNA processing pathway it interacts with proteins like fibrillarin to assist in rRNA maturation. Moreover within the p53 signaling pathway nucleolin regulates the activity of the tumor suppressor protein p53 impacting cell cycle progression and apoptosis. These interactions highlight nucleolin's role in maintaining cellular homeostasis and response to stress.

      Associated diseases and disorders

      The nucleolin protein often shows altered expression in cancers and cardiovascular diseases. In cancer nucleolin's high expression levels correlate with tumor progression and metastasis making it a potential target for cancer therapies. In cardiovascular diseases nucleolin interacts with proteins like VEGF to influence angiogenesis which affects disease progression. Understanding nucleolin's involvement in these diseases provides insight into potential therapeutic targets and interventions.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      3 product images

      • Western blot - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709), expandable thumbnail

        Western blot - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709)

        This data was developed using Anti-Nucleolin antibody [EPR7951] ab134164, the same antibody clone in a different buffer formulation.

        Lanes 1 - 3: Merged signal (red and green). Green - Anti-Nucleolin antibody [EPR7951] ab134164 observed at 100 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

        Anti-Nucleolin antibody [EPR7951] ab134164 was shown to specifically react with Nucleolin in wild-type HeLa cells as signal was lost in Nucleolin knockout cells. Wild-type and Nucleolin knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Anti-Nucleolin antibody [EPR7951] ab134164 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

        All lanes: Western blot - Anti-Nucleolin antibody [EPR7951] (Anti-Nucleolin antibody [EPR7951] ab134164) at 1/10000 dilution

        Lane 1: Wild-type HeLa whole cell lysate at 20 µg

        Lane 2: Nucleolin knockout HeLa whole cell lysate at 20 µg

        Lane 3: MCF7 whole cell lysate at 20 µg

        Predicted band size: 76 kDa

        Observed band size: 100 kDa

      • Western blot - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709), expandable thumbnail

        Western blot - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709)

        This data was developed using Anti-Nucleolin antibody [EPR7951] ab134164, the same antibody clone in a different buffer formulation.

        All lanes: Western blot - Anti-Nucleolin antibody [EPR7951] (Anti-Nucleolin antibody [EPR7951] ab134164) at 1/1000 dilution

        Lane 1: K562 cell lysates at 10 µg

        Lane 2: HuT-78 cell lysates at 10 µg

        Lane 3: HeLa cell lysates at 10 µg

        Lane 4: Jurkat cell lysates at 10 µg

        Secondary

        All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 76 kDa

      • OI-RD Scanning - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709), expandable thumbnail

        OI-RD Scanning - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709)

        We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
        Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com