Rabbit Recombinant Monoclonal Nucleolin antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Images
![Flow Cytometry (Intracellular) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (AB226113), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-epr7952-bsa-and-azide-free-ab226113--flow-cytometry-intracellular-img13759.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (AB226113)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (AB226113), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-epr7952-bsa-and-azide-free-ab226113--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74430.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (AB226113)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (AB226113), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-epr7952-bsa-and-azide-free-ab226113--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132595.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (AB226113)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (AB226113), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-epr7952-bsa-and-azide-free-ab226113--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111167.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (AB226113)")
![OI-RD Scanning - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (AB226113), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-epr7952-bsa-and-azide-free-ab226113--oi-rd-scanning-img85959.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (AB226113)")
Publications
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- Scientific reports 13:53742023Spatial subcellular organelle networks in single cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMythreye Venkatesan,Nicholas Zhang,Benoit Marteau,Yukina Yajima,Nerea Ortiz De Zarate Garcia,Zhou Fang,Thomas Hu,Shuangyi Cai,Adam Ford,Harrison Olszewski,Andrew Borst,Ahmet F CoskunPubMed 37005468
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Expected | Expected | Tested | Expected |
| Mouse | Not recommended | Expected | Expected | Predicted | Predicted |
| Rat | Not recommended | Expected | Expected | Expected | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
8 products for Alternative Version
Target data
Function
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Alternative names
Nucleolin, Protein C23, NCL
Recommended products
Rabbit Recombinant Monoclonal Nucleolin antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR7952
- Purification technique
Affinity purification Protein A
- Dissociation constant
5.8 x 10-11 M
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab226113 is the carrier-free version of Anti-Nucleolin antibody [EPR7952] ab129200.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nucleolin also known as C23 is a multifunctional protein mainly found in the nucleolus with a molecular weight of approximately 100 kDa. It plays a mechanical role in ribosomal RNA (rRNA) synthesis and ribosome biogenesis. Nucleolin is widely expressed in proliferating cells including HeLa culture and shows higher expression in cancer cells compared to normal cells. The nucleolin protein is involved in various nucleolar processes like the modulation of chromatin structure and nucleocytoplasmic transport.
- Biological function summary
The nucleolin molecular weight protein contributes to regulating cellular processes such as chromatin remodeling rRNA transcription and mRNA stability. Nucleolin often forms complexes with various other proteins and nucleic acids enhancing its functionality within the cell nucleus. Its participation in processes like DNA replication and cell proliferation shows the central role nucleolin plays at the cellular level. It influences cellular responses by binding directly to specific RNA or DNA sequences.
- Pathways
The nucleolin protein engages in major cellular pathways such as the RNA processing and p53 signaling pathways. Through the RNA processing pathway it interacts with proteins like fibrillarin to assist in rRNA maturation. Moreover within the p53 signaling pathway nucleolin regulates the activity of the tumor suppressor protein p53 impacting cell cycle progression and apoptosis. These interactions highlight nucleolin's role in maintaining cellular homeostasis and response to stress.
- Associated diseases and disorders
The nucleolin protein often shows altered expression in cancers and cardiovascular diseases. In cancer nucleolin's high expression levels correlate with tumor progression and metastasis making it a potential target for cancer therapies. In cardiovascular diseases nucleolin interacts with proteins like VEGF to influence angiogenesis which affects disease progression. Understanding nucleolin's involvement in these diseases provides insight into potential therapeutic targets and interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry (Intracellular) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-epr7952-bsa-and-azide-free-ab226113--flow-cytometry-intracellular-img13759.jpg "Flow Cytometry (Intracellular) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113)")
Flow Cytometry (Intracellular) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Nucleolin (red) with Anti-Nucleolin antibody [EPR7952] ab129200 at a 1/2000 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nucleolin antibody [EPR7952] ab129200).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-epr7952-bsa-and-azide-free-ab226113--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74430.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling Nucleolin with Anti-Nucleolin antibody [EPR7952] ab129200 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A ready to use HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nucleolin antibody [EPR7952] ab129200).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-epr7952-bsa-and-azide-free-ab226113--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132595.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113)
Anti-Nucleolin antibody [EPR7952] ab129200, at 1/250 dilution, staining Nucleolin in paraffin-embedded Human colon tissue by Immunohistochemistry.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nucleolin antibody [EPR7952] ab129200).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-epr7952-bsa-and-azide-free-ab226113--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111167.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling Nucleolin with Anti-Nucleolin antibody [EPR7952] ab129200 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A ready to use HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nucleolin antibody [EPR7952] ab129200).
![OI-RD Scanning - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-epr7952-bsa-and-azide-free-ab226113--oi-rd-scanning-img85959.jpg "OI-RD Scanning - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113)")
OI-RD Scanning - Anti-Nucleolin antibody [EPR7952] - BSA and Azide free (ab226113)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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