Mouse Monoclonal Nucleolin antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NCL.
Images
![Flow Cytometry - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (AB216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--flow-cytometry-img89692.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (AB216006)")
![Flow Cytometry - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (AB216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--flow-cytometry-img122346.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (AB216006)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (AB216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138431.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (AB216006)")
![Western blot - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (AB216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--western-blot-img63641.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (AB216006)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (AB216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59980.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (AB216006)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human NCL. Database link P19338
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended |
| Cow | Not recommended | Not recommended | Not recommended |
| Cynomolgus monkey | Not recommended | Not recommended | Not recommended |
| Orangutan | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Cow | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Cynomolgus monkey | Dilution info - | Notes - |
Species Orangutan | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg for 106 Cells | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Alternative names
Nucleolin, Protein C23, NCL
Recommended products
Mouse Monoclonal Nucleolin antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NCL.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human NCL. Database link P19338
- Carrier free
- Yes
- Clone number
- NCL/902
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nucleolin also known as C23 is a multifunctional protein mainly found in the nucleolus with a molecular weight of approximately 100 kDa. It plays a mechanical role in ribosomal RNA (rRNA) synthesis and ribosome biogenesis. Nucleolin is widely expressed in proliferating cells including HeLa culture and shows higher expression in cancer cells compared to normal cells. The nucleolin protein is involved in various nucleolar processes like the modulation of chromatin structure and nucleocytoplasmic transport.
- Biological function summary
The nucleolin molecular weight protein contributes to regulating cellular processes such as chromatin remodeling rRNA transcription and mRNA stability. Nucleolin often forms complexes with various other proteins and nucleic acids enhancing its functionality within the cell nucleus. Its participation in processes like DNA replication and cell proliferation shows the central role nucleolin plays at the cellular level. It influences cellular responses by binding directly to specific RNA or DNA sequences.
- Pathways
The nucleolin protein engages in major cellular pathways such as the RNA processing and p53 signaling pathways. Through the RNA processing pathway it interacts with proteins like fibrillarin to assist in rRNA maturation. Moreover within the p53 signaling pathway nucleolin regulates the activity of the tumor suppressor protein p53 impacting cell cycle progression and apoptosis. These interactions highlight nucleolin's role in maintaining cellular homeostasis and response to stress.
- Associated diseases and disorders
The nucleolin protein often shows altered expression in cancers and cardiovascular diseases. In cancer nucleolin's high expression levels correlate with tumor progression and metastasis making it a potential target for cancer therapies. In cardiovascular diseases nucleolin interacts with proteins like VEGF to influence angiogenesis which affects disease progression. Understanding nucleolin's involvement in these diseases provides insight into potential therapeutic targets and interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Flow Cytometry - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--flow-cytometry-img89692.jpg "Flow Cytometry - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)")
Flow Cytometry - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)
Flow Cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling Nucleolin with ab216006 at 1 ug/million cells. Grey: Isotype control; Green: FITC-labeled; Purple: APC-labeled; Turquoise: AF700-labeled ab216006.
![Flow Cytometry - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--flow-cytometry-img122346.jpg "Flow Cytometry - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)")
Flow Cytometry - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)
Flow Cytometric analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling Nucleolin with ab216006 at 1 ug/million cells. Black: cells alone; Grey: Isotype control; Green: AF488-labeled ab216006.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138431.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder carcinoma tissue labeling Nucleolin with ab216006 at 1 μg/ml dilution.
![Western blot - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--western-blot-img63641.jpg "Western blot - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)")
Western blot - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)
All lanes: Western blot - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006) at 1 µg/mL
All lanes: A431 cell lysate
Predicted band size: 76 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59980.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling Nucleolin with ab216006 at 1 μg/ml dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8759.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human uterus tissue labeling Nucleolin with ab216006 at 1 μg/ml dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27146.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin tissue labeling Nucleolin with ab216006 at 1 μg/ml dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82100.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Nucleolin with ab216006 at 1 μg/ml dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8759.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27146.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006), expandable thumbnail](https://content.abcam.com/products/images/nucleolin-antibody-ncl-902-bsa-and-azide-free-ab216006--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82100.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)")
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